Chii chinokurudzirwa S9?

NhanganyayaYakakundwa s9

Yakakurudzirwa S9 ndeye chiropa - yakawana enzyme chidimbu chinowanzo shandiswa mune inefuku uye biochemical research. Yakagadzirirwa kukurudzira enzymes mumhuka, senge makonzo kana hamsters, nemakemikari emakemikari sekumakero 1254, phenobarbital, or β - naphthofone. Ichi chidimbu chakakosha muVitro chidzidzo, kunyanya mu metabolic activation assays, sezvazvinotakura metabolism ye xenobiotic mumuviri wemunhu.

Semunhu anotungamira anotungamira esumvants esumendelogical yezvinhu,FiosciencesUnyanzvi mukutsvaga, kusimudzira, kugadzirwa, uye kugovera zvakakwirira - mhando yemhando yesainzi reagents, kusanganisira yakakurudzirwa S9. Musoro wakagadziriswa muNorth Wales, Pennsylvania, Phosciences yakawedzera rakawanda r & D, nzvimbo dzekutengesa, kuchengetedza, uye kugovera nyika, United States, Europe, uye Europe nyika dzeAsia. Basa redu nderekutsigira vanoongorora pasi rese mumabasa avo esainzi, vanopa kumusoro - Tier biological reagents kuti inokurumidza kubhururuka mukuwanikwa kwezvinodhaka uye necoceology zvidzidzo.

Kukurudzirwa sei smo yakagadzirirwa?

Akakurudzirwa S9 inogadzirwa kuburikidza neiyo tsime - Inotsanangurwa Maitiro:

1. Enzyme induction.
2. Chiropa chinobviswa: Mushure mekununura, chiropa tishu inokohwa uye homogenized.
3. Centrifugation uye kupatsanurana: Iyo homogenized chiropa iri centrifuled kuti iparadzanise iyo S9 chidimbu, icho chine chakakosha metabolic enzymes.
4. Mhando yekudzora uye kuchengetedza.

Zvikumbiro zveApp

Akakurudzirwa S9 ane akawanda ma-plubations paminda yakasiyana yesainzi minda:

• Zvinodhaka metabolism zvidzidzo: Inoshandiswa kuongorora kuti mishonga mishonga ndeye metabolized mumuviri.
• Mutero wemagemity: Anotamba basa rakakosha muAMES bvunzo, kwainobatsira kuziva Mutagenic makomposo nekuenzanisa metabolic activation.
• Carcinogenicity uye kuteedzera kutsvagisa.
• Zvemamiriro ekunze uye makemikari ekuchengetedza ongororo: Inobatsira kuongorora kuchengetedzeka kwemakemikari eindasitiri uye zvakasvibiswa zvakatipoteredza.

Biosciences inokwira kumusoro - kuchena, kukwirira - basa rakakwidzwa S9 zvidimbu, vanogonesa vatsvagiri kuti vabatsire zvakaringana uye vakavimbika mhedzisiro muzvidzidzo izvi.

Michina yekuita yekuita yakakwenenzverwa S9

Akakurudzirwa S9 kunyanya inoshanda kuburikidza:

• Phase i metabolism.
• Phae ii metabolism: Conjugation maitiro (i.e., gluculoronidation, shufation) inosimudzira solubility yemvura yekunyaradza nyore. Nekutevedzera aya metabolic maitiro, akakurumbira s8 kwakakosha pakudzidza zvinodhaka zvekudyidzana uye zvinogona kuve chepfu.

Mhando dzeMakororo S9 Zvikamu

Kune dzakasiyana mhando dzezvikamu zvakakanganiswa s9 zvidimbu zvakagadziriswa kuti zvibodzwa zvakatsvagira:

• Yakamiswa rat chiropa s9: Chidimbu chakanyanya kushandiswa mune genothexicity uye ames bvunzo.
• Induced hamster chiropa s9: Inopa imwe nzira yedzimwe nzira yekuisa system yekufananidza zvidzidzo.
• Jelecler - yakakurudzirwa S9.

Zvakanakira uye zvisingakwanisi kushandiswa kwekushandisa S9

Zvakanakira:

• Inopa iyo yakavimbika system yekufunda metabolism mu vitro.
• Inosimudzira kurongeka mukufanotaura zvinodhaka - kutaurirana kwezvinodhaka.
• Inobata kutevedzera zvinodaidzira zvinodiwa zvekuongororwa kwekuchengetedza.

Zvisingakwanisi:

• Haina kunyatso nyora kuoma kwe metabolism yevanhu.
• Enzyme chiitiko inogona kusiyana zvichienderana nemamiriro ekugadzirira.
• Kugadzikana uye mamiriro ekuchengetedza anofanirwa kudzorwa zvakanyanya.

Maitiro ekusarudza iyo yakakodzera yakakurudzirwa S9 yekutsvaga kwako

Kana uchisarudza zvakakurudzirwa S9, funga:

• Marudzi anobva: Rat kana Hamster ROVER Zvidimbu zvinopa akasiyana enzyme zviitiko profiles
• Inducing vamiririri: Arocler 1254 vs. vamwe vanoti kukanganisa kweNzyme kutaura.
• Chikumbiro Chido.

Pana Pioscience, isu tinopa nyanzvi yekutungamira uye yakagadzirirwa mhinduro dzekubatsira vaongorori vanosarudza izvo zvakanakisa zvidimbu zveS9 zvekuedza kwavo. Zvigadzirwa zvedu zvinokonzeresa mhando dzekutonga kuve nechokwadi chekusimudzira enzyme chiitiko uye kusawirirana.

Mhedziso

Akakurudzirwa S9 chishandiso chakakosha mucocecology, pharmacology, uye yekuchengetedza kwezvakatipoteredza. Nekutevedzera metabolic activation, inobatsira masayendisiti vanoziva muutagenic, carcinogenic, uye makomendi ane muchetura. Semuperekedzi wevanovimbika wezvekuita refalogy, Iphose biosciences anozvipira kukwirisa kumusoro - Hunhu, tsvagiridzo - giredhi rakakurudzirwa S9 kune laboratories pasi rese.

Kuti udzidze zvakawanda nezvezvimwe zvigadzirwa zveS9