Sefapano sa mefuta ea CEREBROLYSIS. le tlhaiso ea CSF) le nts'etsopele ea matrix ea maiketsetso: ho rarolla botlolo ea theknoloji ea theknoloji

1 li-account tsa Iphase

Mesebetsi ea 2 ea 'mele ea lijo tsa Cerebropinal: Bionaanalysis ea Lithethefatsi tsa Boko

Boko bo arotsoe likarolo tse 'ne, ho kopanyelletsa le thelenopphan, Dintebellulose, Cerebellum, le Tsiantem. Mafika likarolong tse fapaneng tsa boko a bitsoa matsoaele, a tletsemokelikeli oa cerebropinal (Csf). Mokelikeli oa Cerebropinal o kenella haholo ho kabo le metabolism ea lithethefatsi bokong le tsamaiso ea methapo ea methapo (CNS).

Likhatiso tsa phapanyetsano ea phaello ea ho atliloeng ea ho atjha, metabolism le tlhaiso-leseling ka bongata ba lithethefatsi. Madi - Likotsi tsa boko (BBB) ​​ke pherekano ea chelete e ikhethileng ka ho nepahala ha lintho tse entsoeng ka mokhoa o kotsi, e fokolisang ho kena mali maling a bokong bo kena ka har'a boko. Ha BBB e fetola pherekano ea lithethefatsi, ho lemoha hore na ke ho lemala ha mali ho ka 'na la sitoa ho bonahatsa khatello ea lithethefatsi bokong. Ho sebelisa feela ho sebelisa litlhare tsa litlhare tsa litlhare tsa litlhare tsa litlhare bakeng sa khatello ea methapo ea lithethefatsi tse bakoang ke liphoofolo tse bakoang ke liphoofolo tse bakoang ke liphoofolo tse bakoang ke liphoofolo tse bakoang ke liphoofolo tse bakoang ke liphoofolo. Ka hona, maemong a mak'ro, maemong a tlhaho a boko a ho fepehileng kapa a Cerebropization a ne a sebelisoa haholo bakeng sa lithuto tsa pharmacokinetic bokong.

3 Bohlokoa ba Tlhahlobo ea STREBROYSSYSILY NTHAPENG E NENG

Khokahano ea protheine ea CSF e tlase haholo, mme khatello ea eona ea lithethefatsi e sebelisoa e le setšoantšo sa mahala bakeng sa lithethefatsi tsa mahala tsa sekolo. Leha ho le joalo, ts'ebetso ea CSF e ntse e tsamaea butle ho feta ea madi, e hlahisang moqoqo o sa lekaneng oa liphoofolo. Ho latela sebaka sa ho fallela / nako ea tsamaiso ea nako le tsamaiso ea tsamaiso CSF ​​e ka fapana haholo.Mohlophisi oa CEABROEY (CSF ea Maiketsetso, acsf) ke tharollo e hanang sebopeho le ts'ebetso ea mokelikeli oa tlhaho oa tlhaho, 'me o ka lekola data ea phallo ea phapanyetsano ea vitro. Ntle le moo, metsi a maiketsetso a maiketsetso a ka sebelisoa ho lekola litlamorao tsa ona ho Yo Bode le liprotheine.

4 Mathata a tlhahlobo ea cerebropusis le tlhoko ea matrix e se nang letho

4.1 Meeli ea boitšoaro / e nang le litheolelo tsa ho fumana mokelikeli oa tlhaho oa Cerebropinal

Ho fumana metsi a tlhaho a Cerebrosinal ho ntse ho le joalo, a le litlamo. Batho ba phetseng hantle ba hloka ho fumana metsi a Cerebropinal ka litloholo tsa maumbar kapa tsa mofuta o mong o hanang ho hapa baithaopi ba bophelo bo botle. Sebelisa metsi a maiketsetso a maiketsetso a maiketsetso /mokelikeli oa cerebrosinal(a maiketsetso /CSF ea CSF)) Matrix sebakeng sa maikutlo a tlhaho, ho qoba ho hlahlobisisa ka mokhoa o ikhethileng ha u ntse u boloka litšobotsi tsa Matrix (joalo ka protheine e tlase ea Matrix (e kang protheine e tlase ea Matrix (joalo ka protheine e tlase ea Matrix (joalo ka protheine e tlase ea Matrix (joalo ka protheine e tlase ea Matrix (e joalo ka protheine e tlase ea Matrix (joalo ka protheine e tlase ea Matrix (e joalo ka protheine e tlase ea Matrix (joalo ka protheine e tlase ea Matrix).

Litlamorao tsa Matrix tse bakoang ke phapang ea batho ba bohlokoa

Mefuta ea mefuta / motho ka mong e le li-profeshe tsa protheine (15 - 100 mg / Liphello tsa phosphonion Ka ho etsa hore motho ea tloaetseng ho etsa slubrosi a maketsetso / e fetisitsoeng ea cerebropinal le litekanyetso tsa mefuta e fapaneng ea li-propente tse fapaneng, LC - Lithepu tsa MS / MS - Liphapang tsa Bolulo tsa BAND. Hape hoa khoneha ho lokisa karolo ea mobile (joalo ka hilic chromatography) kapa u tsamaise nako ea ho boloka thepa ho tsoa ho likarolo tse fapaneng tsa matrix.

4.3 Tšusumetso ea liphetoho lihlopheng tsa tlhaho tsa Matrix ka tlhahlobo ea maemo a lefu

Mofuthu oa Cebrolinal o ka ba le liprotheine tse sa tloaelehang (tse kang ho fokotseha ha lefatše.

5 Frafs Mefuta ea Cerebropinal

5.1 Motseng oa batho ba Cerebrolinal(CSF ea motho)

Mokelikeli oa batho oa Cerebroke matrix e tloaelehileng ea mokelikeli oa Cerebrosinal Matrix, empa mefokolo ea boitšoaro e lebisa ho hloka pelo ea disampole ea tlhaho; Li-profete e tlase (15 - 45 mg / dl)

5.2 CynomolGus CynomolGus Cysolgunal Slubronion (Monkey Csf / NHP CSF)

Cynomolgus Monkey Cerebropulke ena ea batho ba ikhethileng e haufi haholo le batho, e nang le liphapang tsa batho ba nyane, e loketse tlhahlobo ea PK / PD le polokeho ea polokeho eaLithethefatsi tse tloaelehileng tsa CND, ke mohlala o ratoang haholo bakeng sa lithethefatsi tse ngata tsa ho sebelisa lithethefatsi.

5.3 rhesus cerebropinal mokelikeli (monkey csf / nhp csf)

Rhes Cerebropinal mokelikelie tšoantšoa le monkey ea cynomolgus, e na le litšobotsi tse matla tsa melaetsa ea neurommuni 'me e loketse maloetse a amanang le neuroinfal kapa lithethefatsi tsa ho itšireletsa mafung.

5.4 BEAGLE LOGLE CEREBROY HUMPER (Begle Ntja Csf)

Blugle Cilrebrolinal Sluidke mohlala o phahameng oa prothefo, o loketseng ho lekola mali - bokoang bokong bo phela le ho ithuta ho sebelisa melabolism ea lithethefatsi.

5.5 sd rat cerebropinal mokelikeli (Rat csf)

SD Rat Cerebropinal mokelikelike metsi a manyane a ntlafatso ea Cerebropinal, a e-na le ho hlahlojoa, ho kenya chelete e tlase le ho sebelisa mefokolo e bonolo, e sebelisoang haholo thutong ea lefu la neurodegneratinse.

5.6 Mouse cyrebropinal mokelikeli (CSF CSF)

Mouse Cerebropinal mokelikeliNa mohlala oa bohlokoa oa liteko tsa tlhahlobo-leseling, haholo-holo ho etsa lipatlisiso tsa mafu (joalo ka lefu la mafu a Alzheimer) le tsamaiso ea li-parkin.

5.7 Mitipig Cerebropinal Fluid (Minipig CSF)

Minigue Cerebropinal Fluid 'S Anatoil Greomical Sebopeho se lumella Sampling e pheta-phetoang, e etsang hore e loketse nako e telele - Palo ea Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa Lipapali tsa phano. Leha ho le joalo, sebopeho sa phospholilids ha se tšoane le seo, 'me khetho ea mokhoa o hloka ho netefatsoa.

5.8 Rabbit cerebropinal mokelikeli (Misbit Csf)

Misbit Csf ke e 'ngoe ea mefuta e tloaelehileng ea liteko tse sa sebetseng hantle, haholo-holo mafu a lithethefatsi, boholo ba methapo ea kutlo (li-cens) tse mamellang, moo e nang le menyetla e ikhethang.

Sephetho

Tlhahlobo ea mokelikeli oa Cerebropinal (CSF) ho kenya tšebetso ea lithethefatsi bohareng, ho fana ka maikutlo a lithethefatsi ho ea sebakeng sa lithethefatsi - Boko ba boqapi tsamaisong ea methapo ea kutlo. Leha ho le joalo, tšebeliso ea csF ea tlhaho e tobana le mathata a sebele, ho kopanyelletsa le litekanyetso tsa boitšoaro, bo kenang ho sebopeho sa matrix, le lintho tse fetotsoeng ho matrix. Mefokolo ena e hatisa bohlokoa bakeng sa li-matric tsa ACSF, tse thusang ho ba le likhatello tsa boitšoaro le tsa tekheniki le botekheng le botekgeniki.

LC - MS / Mme o hlahile e le sesebelisoa sa thuto sa CSF, se fana ka maikutlo a mangata le ho ba bapalaki ba tlaase haholo. Leha ho le joalo, litlamorao tse khantšitsoeng ke phapang ea protheine, lipid le litaba tsa motlakase ho pholletsa le mefuta - u hloka ho itokisa ha sampole ea limpho le li-chromotographic. Matric ea Maiketsetso, e lumellanang ho etsisa maemo a bophelo bo phetseng hantle le a bo-ramahlale, ho matlafatsa matla a Alzheimer le ho theha papiso - Ho matlafatsa CrolmageSons.

Har'a mehlala ea liphoofolo, cynomolgus monkey csf e tšoana hantle le CSF mme e khethoa bakeng sa tlhahlobo ea hau ea li-csf e loketseng. Mefuta ea Roden (e.g., SD rats) Fana ka litšenyehelo - e phahameng, phahameng -

Qetellong, ho kopana ha mefuta ea mefuta ea CSF ho fana ka li-matfo tsa csf tse ikhethang tse nang le LC - Liteko tse nepahetseng le tse fetohang kelellong. Tsoelo-pele ea nakong e tlang e lokela ho tsepamisa maikutlo ho ho nchafatsa mekhatlo ea CSF ebe e u sitisa lipalesa tsa Belinalyttical.

Mantsoe a Bohlokoa: Mollo oa Mekete ea Cerebropinal, mokelikeli oa maiketsetso oa maiketsetso, CSF ea maiketsetso CSF, CSF CSF, lintja tsa lintja tsa maballo, ntja ea SD, CSF

Vite: Ban Wei - Kang, Yang ZHI - hong. Maano a lipatlisiso le mahlale a ka pele a chankaneng Intracrebral Paricakitics. Machaena a Liphatlalatso tsa China, 2023, 39 (9): 1607 - 1612.