Hepatocytes ha li-metabolism le sirna lithethefatsi: ho lekola litsamaiso tsa Model

Tlhokomeliso: Liatocytes tsa ho emisoa li hloka ho sebelisoa le thaw e bohareng; Li-hepatocytes tse se nang kelello li hloka ho sebelisoa le thaw bohareng, Semelo sa mahareng, mechine e hlophisehileng ka har'a poleiti e bohareng le collate. Phokotso ea Cyp e hloka horerna Induction Assay Kit hape.

Hepatocytes

HepatocytesNa lisele tsa mantlha tsa sebete, li bapala karolo ea mantlha ho metabolism, sotoxodation, tlhahiso ea banyane, le protheine Syntthesis. Li theha motheo oa ts'ebetso ea sebete, o iketsa eka ke mekhabiso e ka sehloohong ho feto-fetoha ha metsoako e fapaneng le ea Exogenus hore e be kotsi le ho tsamaisa metabolism ea lithethefatsi le chefo. Tsebo ena e makatsang ea ho phela ka makhetlo a ho sebetsa lik'hemik'hale ho ba entse ba sa hlokeng likomik'hale maemong a kenyelletsang litselana tsa lithethefatsi le linoko tse fapaneng.

Litereke tsa mantlha tsa mantlha

Thato ea mantlha e bua ka lisele tseo tse ncha tse ikhethileng ho tsoa ho lisele tsa sebete - hore na liphoofolo - ntle le lefu la vitro. Hobane lisele tsena li boloka boholo ba mesebetsi ea enzzymatic le lits'ebeletso tsa 'mele tseo ba nkoang e le molao oa khauta lithutong tse hlahlojoang - LITLHAKISO TSE KHOLO. Tšebeliso ea bona ea lipatlisiso ke pivotal ho utloisisa botsitso ka bobeli ba metabolisic le bokhoni ba ho fetoha ha bona pele ba ne ba tsebisoa tsamaisong ea likokoana-hloko hang-hang.

Li-hepatocytes tsa li-Hepatocytes le li-Ipatocytes tsa ho emisoa

Ka lebaka la lits'oants'o tsa liteko, li-hepatocytes li ka aroloa ho latela maemo a tsona a moferefere. Likhechana tse hlakileng ke tse lumellanang le sehlopha sa setso sa setso, se lumellang sehokelo se tsitsitseng le morphologis tikolohong ea vivo. Sehokelo sena ha se ts'ehetsa sebopeho se tlotsitsoeng feela empa se boetse se ts'ehetsa nako e telele - lithuto tsa nako ea thekiso ea biochemical e joaloCyp450 enzyme indection. Ka lehlakoreng le leng, lithaelese tsa ho emisoa li bolokoa ho se seng sa ho latela, hangata ho sebelisoa ho tikolohong ea cellular le tlhahlobo ea metaboli li hlokahala. Setso sa ho emisoa ho tsamaisa lisele tse tšoanang ho etsa litlokotsi tsa liteko, ho li etsa hore li be le mokhoa o potlakileng oa ho tsitsitse metabolic.

Li-hepatocytes tse hlakileng

Li-hepatocytes tse hlakilengke lisele tse ikhethileng tse ikhethileng lijana tse koahetsoeng kapa tse ikhethang tsa setso. Koakelo ena e lumella lisele tsa ho li jala, li tsamaisa mabitso a lingobo, li tsamaisa tse ngata Vivo - Lits'ebetso tse fetang matsatsi a mangata kapa libeke tse 'maloa kapa libeke tse ngata. Hobane lisele tsena lia latela, li ka sebelisoa ho shebella likarabo tse sa feleng, mokhoa oa ho inehela oa cyp450 ha nako e ntse e tsamaea. Lifofane tse hlakileng li boetse li boloka sele e ngata haholoanyane - Puisano ea Sell - Ho Buisanang Matrix Matrix Mefuta e 'ngoe le e' ngoe - ebang ke motho, e seng a le motho, ea haufi le batho ba bangata ba hlokang ho ba le maemo a hlakileng a ho beha mabaka ka ho fetisisa.

Libaka tsa ho emisoa

Ka papiso,Libaka tsa ho emisoali sentsoe ho motho ea sa kopaneng le ho boloka morology ea pote. Liteko tsa ho emisoa li loketse ho ba le bokhutšoanyane - liteko tsa nakoana, tse fumanehang lithutong tsa mahaeng kapa tsa enzymis tsa Enzyme Kintetics. Kaha lisele li se ke tsa qokoloa ke maemo a matric, li ka hlophisoa ka potlako le ho sebelisetsoa liteko tse sebetsang ntle le ho lieha ho hlokahalloa ke sele. Litso tsa ho emisoa ke ho na le lintho tse ngata ha li bapisa mefuta eohle, joalo ka ha li lumella batho ba itšehang ka potlako ho batho, litopollo le mefuta e meng.

Botsitso le botsitso le hepatotoxicity

Botsitso ba metabolicBoemong ba lipatlisiso tsa TiPocyte bo bua ka bokhoni ba molek'hule, hangata e le mokhethoa oa lithethefatsi, ho hanela phetoho ea biochemile ka li-enzyme tsa biochemical. Ho etsa lipatlisiso ho ba le botsitso le li-hepatocytes ho etsa hore bafurase ba bolele esale pele halofo - bophelo le lihlahisoa tse nyarosang tsa lithethefatsi. Boemo ba metabollic profile e ka khetha ho sebetsa ha eona feela feela empa le polokeho ea bona, joalo ka polokeho ea eona ka linako tse ling e ka bonoa ka khubung ea motsoali.

HepatotoxicityKa lehlakoreng le leng, ka lehlakoreng le leng, e akaretsa litlamorao tse mpe tsa lintho tsa lik'hemik'hale lisele tsa sebete. Tšebeliso ea li-vapacytes tsa mantlha tsa mantlha le tse tsoang lithutong tsa chefo ho thusa ho hlakisa mekhoa e lebisang ho lemala la sebete. Sena se bohlokoa hobane sebete, e le setho sa bo-metabolic se bohareng ba lik'hemik'hale ho tsoa ho lik'hemik'hale ka bobeli le li-ageletical. Boithuto bo hlalosang lisele tsena li khona ho bona mekhoa ea tšenyo ea Cell kapa ho fana ka leseli liketsahalong tsa pele tsa likotsi tsa bongaka ka mokhoa oa bongaka.

Ho itšireletsa motho ke karolo e 'ngoe ea bohlokoa e hokahantsoeng le ts'ebetso ea lefutso, e bua ka ts'ebetso eo lintho tse ling li eketsang maikutlo a lithethefatsi - metabomo. Ntho ena e 'ngoe e bohlokoa haholo joalokaha e ka lebisa metabolism e eketsehileng ea ho sebelisa metabolism ea Co - lithethefatsi tse hlahisitsoeng, ha li fokotse tšebetso ea bona kapa ho eketsa tlhahiso ea metriboolite tse kotsi. Patlisiso e sebelisang li-hipatocytes hangata e ikemiselitse ho hlahloba mekhoa ea litlhare tse laolang litšebelisano tse rarahaneng lipakeng tsa li-xen hoobolic tse fapaneng le metabolic metabolic metabolic metabolic metabolic metabolic metabolic metabolic.

Hepatic Sirna tlisoang le maano

Liphetoho tsa morao-rao ho RNA Straferics (RNAI) Bashebelli ba kentse maano a senaPUSELETSO EA HEPATIEDho sebelisa rnas tse nyane tse kenang lipakeng tsa li-surnas (Sirnas). Mokhoa oa bohlokoa o kenyelletsa ho kopanya limolek'hule tsa Sirna Molecules tse nang le n - acetylgalactosamine (Galnac), e fanang ka matla a ho khetha ka li-hepatocytes ka nako ea AsiaglickcoproteinASGRS. Asgpr e hlahisoa haholo ka ho lekana le li-hepatocytes mme o phetha karolo ea mantlha ho se kahare ho naha eaGalnac - SirnaE kopanya, e e etsa tsela ea bohlokoa ea sebete - Ho tsamaisa lithethefatsi ka ho Khethehileng.

Lipuisano tsa vitro li tloaetseThrost Hipacyte Sirna TransnaMekhoa ea ho lekola katleho le ho hlaka haThoriso ea Sirna Gapac Hepatocyte. Mekhoa ena e lumella bafuputsi hore e ithute litlamorao molemong oa ho holisa pele le ho ntlafatsa maikutlo a holisitsoeng le a le mokhanni.

Ka kakaretso, hepatic

Mefuta ea Mefuta ea Hepatocyte

Hupatocytes ea Batho

Li-hepatocytes tsa motho tse hlakileng li bohlokoa bakeng sa ho bolela metabolism ea lithethefatsi le chefo, e fana ka matla a maholo. Ho latela moea oa bona ho khethehileng ho lumella tokiso ea metabolic enzyme indection, Karolo ea I / II Metabolism le ea thekiso ea thepa.

In a e mong - sebopeho se kopaneng,Ho emisoa Hupatocytes ea Batho Ka potlako araba li-Xen hookoobios, li etsa hore li lokele tlhahlobo ea metabolism, lithuto tsa Boloetse le khukhuno.

Eseng - lintsicyte tsa batho ba ka sehloohong

Ho tšoana le ho 'na ha batho ho etsaMonkey Clunomolgus Monkey HepatocytesmmeMonkey Monkey Monkey Liitchtes E le bohlokoa bakeng sa liphallelo tsa pale ea batho ba nang le liphetho tsa batho. Li sebelisetsa ho lekola metabolism ea lithethefatsi, mokhoa oa enzyme, le mefuta ea Enzyme, le Mefuta ea Enzyme - Litaba tse ikhethang tsa polokeho.

Cynomolgus cynomolgus Monkey HepatocytesmmeDouspesion Rhes Monkey Liitky Etsa lithunya ka potlako le tse phetetsang likarabo tse matla, tse matla likarabo tsa nako - ho na le lithuto tsa nako ka mepamo e hlakileng.

Canine Hepatocytes

O sebelitse haholo-holo ka lipatlisiso tsa bongaka tsa bongaka,Li-hepatocytes tsa ntja tse hlakilengmmeLi-hepatocytes tse hlakilengMiphic in VIVO Liver Exseygygy ho ithuta lithethefatsi - Ho lemala hampe le metabolism ea li-pharmaceuts tsa liphoofolo.

Tsena li fana ka lithuto tse potlakileng tsa metabolism 'me li sebetsa mathoasong a pele - ho hlahlojoa ha litefiso tse tsoang' meleng oa ho emisoa.

Spague - Dawley Rat Hepatocytes

TsenaSPRAUGE - Dawley Rat Hepatocytesli arotsoe ho sprague - Likhoto tsa Dawley 'me li letsoa ka sebopeho se kopaneng kapa se hlakileng. Mokhoang o fokang o fokang, lisele li hokela sejana sa moetlo, se ba lumellang ho sebelisana 'moho le ho boloka sebete sa linako tse itseng bakeng sa nako e itseng. Sebopeho sena se loketse ho ithuta bolelele -

Ha e bolokoa ho ea se ke keng ea lekanngoa,SPPERION SPRAUGE - Dawley Rat Hepatocytesli sebelisoa haholo bakeng sa bokhutšoanyane - liteko tsa nako. Sebopeho sa ho emisoa se nolofalle lithuto tse matla tsa metabolis le lingoloa tsa enzyme Kinost. Mokhoa ona o na le molemo ha o bapisa likarabo tse mpe tsa metabolic lipakeng tsa mefuta kapa maemo a fapaneng.

ICR / CD - 1 mouse hepatocytes

Nchele ho ICR / CD - MIC ea 1, MIL Icy's cd - 1 mouse hepatocytesKhomarela sejana sa moetlo, se ba lumellang ho matlafatsa tšebetso ea bohlokoa ka lebaka la ho tšoarella. Bafuputsi ba rata mokhoa o hlakileng oa ho Icy / CD - 1 Mouse HEPAtocytes e 1 ea Mouse

Ho fapana le moo, ho emisoa ICR / CD - 1 Mouse Hitmellocytes ntle le ho boloka morōpi e lenngoeng hantle - lithuto tsa nako.Ho emisoa ICR / CD - 1 mouse hepatocytesE lumella tlhahlobo e potlakileng ea likarabo tsa metabolic 'me e na le thuso e ngata ka tatellano ea pele kapa ha e phahameng - Ho hlokahala tlhahlobo ea ho kenya. Tšebeliso ea li-Ipatocytes tsa ho emisoa ho ICR / CD - 1 MIE e thusang ho theha profiles kapele le maemo a mang a liteko.

Feline Lipatocytes

Sebopeho se hlakileng se na le thuso e khethehileng bakeng sa khale - Lithutong tse shebaneng le mafu a Feline Liver, lithethefatsi - Ho lemala hampe, le mathata a mantlha a metabolioni a tobileng ho likatse,Li-Hepatocytes tsa Cat Hepatocytesfana ka mohlala o makatsang. Kamano ea bona e boetse e ntlafatsa maemo a bokhopo le a sebetsang, a ba etsang mohlala o ratoang ka li-veterinary Meganation

Sebopeho sa ho emisoa ha litlhahiso tsa bana ba seng ba celpes ho XenOodios, moo ho haellang ha tšoaetso e nyenyefatsang ho lieha ho arajoa nako e telele - litso tsa bona.Li-Hepatocytes tsa ho emisoa ka hona, ba bohlokoa bakeng sa ho tsebahatsa liphetoho le liphetoho tsa pele tsa biochemical le karabelo e fosahetseng ea ho pepesetsoa metsoako e meholo.

Li-Hepatocytes tsa likhoho

E le baemeli ba mefuta ea Avian,Li-Hepatocytes tsa khoho tse potilengli sebelisoa lithutong tse bapisang ho utloisisa phapang ea puso ea ho sebetsa ka mesebetsi ea sebete, haholo-holo tlhahlobo ea liteko.

Li-Hepatocytes tsa ho emisoaE thusa ho potlaka, ho na le lithuto tsa mantlha tsa mokhabo oa sebete tsa sebete tsa linonyana mme li ka ba hahoa ka ho toba le balekane ba liphoofolo.

Mefuta e meng

Bakeng sa mefuta e meng, Pleteable Minigig Hepatocytes, Defepesion Minipig Hpatocytes, Li-Hepate tse potelletseng tsa New Zealand Hepatocytes, Eketsehileng New Zealand Hepatocytes Tsohle ke lintho tse tloaelehileng tsa ho bolela esale pele le ho hlalosa lithethefatsi - ho sebelisa lithethefatsi lithethefatsi le ho ithuta ka mokhoa o hlakileng oa lithethefatsi.

Sephetho

HEETOCTES PHELA MOLEMO OA HO SEBELISA KOTSI KA Meabolism, timetso, tlhahiso ea proten Syntthesis. Sehlooho sena se totobatsa phapang pakeng tsa likhechana tsa mathomo le mefuta ea tsona ea litso tse hlakileng le tse emisitsoeng likotlo tse fapaneng tsa liteko. Lits'ebetso tse khopisang, ka matla a tsona a khopisitsoeng, a ipabola ka lebaka la ho ithuta litlatsetso tse sa feleng le tse telele - ho pepesetsoa maemo a ho pepeseha ka potlako le a junifomo bakeng sa litlhaloso tse matla. Ntle le moo, tlhahlobo e fetela ho mefuta ea mefuta ea mefuta e fapaneng, ea ho boloka li-metabolism-li-Hepatotoxicity tse kang RNA - LITLHAKISO TSA HO SEBELISANG TSA BOPHELO. Kakaretso ena e felletseng e bonts'a karolo ea bohlokoa ea lipatlisiso le kholo ea pharlical, ho fana ka leseli la polokeho ea li-sebete le katleho.

Mantsoe a bohlokoa: Li-Hepatocytes, Hupatocytes, hepatotoctor, Heatyme Cynomolgus Monkey Hepatocytes, Rhes Kholot ea Hepatocytes, e hlakileng Hepatocytes, CD - Heatotocytes, Phahameable Fepatocytes, Phahameng ea Phahameng ea Kutsha Hepatocytes, li-sprague - Dawley Rat Hepatocytes, Deperient Peotocytes, Desuters New Zeperictes, khoho ea ho emisoa Hepatocytes,Asgr, galnac - Sirna, Murna oa Hipacyte Sirna Translac hepatocyte,Phepelo e lebisitsoeng.