Utloisisa karolo ea lits'oants'o tsa sh tse susumetsang tsa S5 tsa lithuto tsa metabolism tsa metabolism

Mefuta ea vitro e se e le bohlokoa haholo litabeng tsa sejoale-joale le mericacokitics, haholoholo ha u lekola hore na komiti e sebetsa joang ka mekhabiso pele e tsoela pele ho tsoela pele. Sesebelisoa se seng se amohetsoeng haholo lithutong tsena ke karolo e susumetsoang ke S9, boitokisetso ba subcedente bo tsoang ho li-enzyme tsa mindomal le li-cystorac tsa microsolic. Likaroloana tsena li lumella tlhahlobo e sebetsang ea metabolism ea Xenbiotic, mesebetsi e kenang ea enzyme le metabolic ea metaboli ea liteko.

TheS9 Karoloana ea S9Hangata e tsoa ho lisele tsa lisenshene tsa rodents le li-hamsters tse tloaelehileng haholo tsa marang-rang tse tloaelehileng - li tšoaroa ka polelo ea enzyme ho ntlafatsa polelo ea enzlic. Motsoako oa SCA o ile oa bakotsi oa SCA o ile oa ba botsoa ba kang Cytochrome P450 oxidises enzlation Ena - Boteng ba enzyme bo bohlokoa ha bo lekola mekhoa e rarahaneng ea metabolic ka setulong sa laboratori.

Karolo eaLi-rat sebete s9

Li-rat sebete s9Na lintho tse fapa-fapaneng tse sebelisoang khafetsa, haholo-holo ka liteko tse fapaneng joalo ka assay kapa tlhahlobo ea microcleus. Likaroloana tsena li hlophiloe hangata pele - Ho sebetsana le mehanya ka aroclor 1254, Phenobarbital, kapa β - Naphthofne. Aroclock 1254, haholo-holo, e sebelisoa haholo ho hlahisa aroclor - E khabisitsoe ka S9, e fanang ka litheko tsa bolokolohi ba P450 isozymes. Tsena li bohlokoa bakeng sa ho panya ha sebete sa sebete sa sebete se fumanoang Vivo, e fanang ka temohisiso ea tlhaho ea hore na khimik'hale e ka fuoa monyetla oa ho ba mochine oa muvonic kapa o chefo.

Likopo tsa Aroclor - O ILE A SEBELISANG:

• Ts'ebetso ea Mammalian Metabolic

• Ho etsa lipatlisiso ke pele ho li-biotransform tsa "lik'hemik'hale tsa indasteri

• Ho bolela esale pele metabolism ea lithethefatsi ka ho ithuta li-analogs tsa sebopeho
  
  
  
  

Nako ea ho sebelisaLi-hamster tse susumetsang ka lila

Le ha likhoto li na le melao-motheo e mengata ea matsoele,Li-hamster tse susumetsang ka lilae fana ka profil e fapaneng ea enzyme. Metsoako e meng e ka ba metriborients ka tsela e fapaneng ho latela mekhoa ea enzyme, e etsang hamstery e batlang data ea metabolic ka likhoto ha li lekane. Batho ba bangata ba sebelisa boholo ba lipatlisiso mme ba thusa ho fokotsa lijalo tsa bohata kapa ho feta - Litlhaloso li bakiloeng ke Mefuta ea leeba.

Ho nahana ka S9 tšebeliso ea S9

Ha ba khetha tsamaiso e loketseng ea S9 e loketseng, bafuputsi ba lokela ho nahana ka:

• Mofuta oa compond (pro - lithethefatsi vs. Tumellano -

• Mofuta oa li-enzymes tse hlokoang (oxidation vs. Conjugation e kholo ka ho fetisisa)

• Litebello tsa tsamaiso (e.g.

• Lintho tse ngata tse feto-fetohang le tšebetso ea enzzymatic ea s9 e sebelisitsoeng
  
  
  

Iphase: Tšehetso e tšepahalang bakeng sa S9 - Patlisiso e thehiloeng ho Metabolic

At Iphase, re utloisisa ho rarahana ha pele - ho hlahlojoa ha liteko le ho hlahlobisisa tlhahlobo. Re fana ka lits'oants'o tse tloaelehileng tsa SCAN SCA SCA SCA SCA S9, li ile tsa susumetsoa ka lehare la Hamters Sehlopha se seng le se seng se lekoa bakeng sa ketsahalo ea enzzymatic le Lota - To - Lota e Tšoanela, Ho Na ho Etsa'ng Hore e Liphetele Tse Fetohang lithutong tsa vitro.

Ke na le mekhatlo ea tikoloho ea phaello, tikoloho le ea thuto, Iphase e ntse e le molekane ea tšepahalang oa ho fana ka lits'oants'o tse mabapi le litlhoko tsa kamora ho etsa lipatlisiso. Lisebelisoa tsa rona tsa S9 li hlahisoa ho tsoa ho liphoofolo tse hlohlelletsang ka hloko, 'me li sebedisoa ho latela litataiso tse amoheloang ka hloko, li netefatsa hore ho latela litlhoko tsa liteko tsa kajeno tsa metabolism.