Tlhahlobo e bapisang ea lisele tse patisaneng tsa mohloa tsa mohloa tsa mohloa tsa mohloa tsa bosholu

ILihlahisoa tsa Mokha

Lisele tsa Mali oa Mali oa Mollo oa Mollo (PBMCS)

Lisele tsa Mali oa Mali oa Mollo oa Mollo (PBMCS)ke batho ba bangata ba lisele tsa 'mele ba fumanoeng maling a nyonyehang, ba khetholloang ke nyutlelie e le' ngoe. Ba kenyelletsa lymphoctes - joalo ka lisele tsa T, B lisele le Killer (NK) Hangata li-PBMMCS li arotsoe ka mokhoa o hlakileng o sebelisang media o nka media, o arohanyang litsoi se thehiloeng ho buoyanchoeng. Tsela ena e seng - mokhoa o hlaselang o lumella ho fihlella habonolo ha likarolo tse thata tsa 'mele, ho etsa hore PBMMCS tsa bohlokoa ba ho etsa lipatlisiso le nts'etsopele ea kalafo ea Inteloutic.

PBmc isolation

Boholo, moichaba oa morphology oa lisele tse nyane tse sa tsoakoang li fapana ho lisele tse ling maling a se nang meeli. Matla a khoheli a lisele tse khubelu tsa mali le litlolo tsa polymmocucucles tse fetang 1.080, ke tsa lisele tsa motho ka mong li ka hakana 1.030. Ka hona, phapang pakeng tsa matla a khoheli a fapaneng a mali le lisele tsa motho ka mong li ka sebelisoa ho arola lisele tse fapaneng tsa mali ho lisele tse fapaneng tsa mali. Ficoll Dentity Centrifiging ke mokhoa o sebelisoang haholo oa ho sebelisaHo itšehla thajana ea PBMCle tlhoekiso. FOCOLL ke Metsoako e Actrose, ea sa nke lehlakore Ka hona, hangata e sebelisoa e le tharollo ea karohano bakeng sa karohano ea PBMC. Lisebelisoa tsa Erythrocytes le li-granulocy li na le matla a khoheli a lekaneng le ho teba ka tlase ho tube ka mor'a ho ts'oaroa; Li-lymphocytes le li-monocytes li na le matla a khoheli a itseng, 'me karolo e nyane ea lisele e ka emisoa le tharollo ea karohano ea karohano. Ka ho sheba lisele tsa halofo e tšoeu ea membrane ka metsing a lijalo tsa ho arohana, i.E., PBMC, e ka aroloa maling a linoko.

PBMCS LITLHAKENG TSE KHOLO

Ho batho bao e seng -misotes ba batho, haholo litšoene tsa cynomolgus le moskey ea Rshes, Patlisiso e bohlokoa haholo ka lebaka la 'mele eo ba e arolelanang le batho. Patlisiso e sebelisitsoengcynomolgus monkey pbmcmmeRhessus monkey pbmce thusitse lithuto tse qaqileng tsa mecha ea 'mele, le lithuto tse ngata li sebelisa bukana ea linkey PBMC li ntlafalitse menyetla ea bona ea phetolelo ea ho ameha. Ts'ebeliso ea Monkey PBMC Phuputsong ea lihlahla, ho hlahloba sethethefatsi le ho itšireletsa mafung, le ho itšireletsa mafung e se e le liphuputso tsa sekhukhuni le tsa bongaka.

Ka mokhoa o ts'oanang, lipatlisiso tse amanang lentja pbmcba thusitse haholo pateiso ea lefu la tsoekere. Boithuto bo sebelisa lintja PBMC li bonesitse litsela tsa karabelo ea ho itšireletsa, ha ho ntse ho etsoa lipatlisiso mabapi lecanine pbmcE boetse e supa tšebetso ea ho itšireletsa mafung ea batho. Tšimong ea maloetse a Zoootic, ntja ka bobeli ea PBMMC le Patlisiso ea Canine PBMC e fane ka data e matla ea lisente - Ntlafatso ea kalafo e hlophisehileng. Ts'ebeliso ea nako e pheta-phetoang ea ntja ea PBMC le Canine PBMC ho feta ha e atleha e le mokhoa oa mohlala.

Mefuta ea metsamao e kenyeletsangrat PBMCmmemouse pbmc, ka tloaelo ka setso e ne e le pele ho lipatlisiso tsa ho ipheteloa. Patlisiso e sebelisa rat PBMC e fane ka pono e qaqileng maemong a sa feleng a ho itšireletsa mafung, le tšebeliso e kholo ea mouse e bile ea bohlokoa bakeng sa liphallelo le tšoaetso le ts'oaetso. Bafuputsi ba ntse ba tsoela pele ho itšetleha ka rat pBMC le teuse pbmc ho hlahloba lits'ebetso tsationtion tsa imminononononononononoratoring, e etsang mefuta ena ea bohlokoa. Ho feta moo, ho kopana hahamster pbmcLibakeng tsa liteko li ekelitse tšobotsi e 'ngoe ho li-subter tsa inome, e le Hamster PbmC e fana ka mekhoa ea lipale le maano a bophelo le a sa sirelehang. Ka hamster pbmc e tšehetsang lipatlisiso ka rali rat pBMC le mouse pbmc, medes ea meden

Mefuta e kholo ea liphoofolo e boetse e lokisitse tšimo, leMinipig PbmcHo bapala karolo ea bohlokoahali ho bakoang ho pheha lithuto le likopo tsa bongaka. Ts'ebeliso ea Minipig pbmc e netefalitse molemong oa ho nchafatsa meriana ea ho nchafatsa, moo Minipig Pbmc e fana ka sethala se nepahetseng sa mmele bakeng sa ho hlahloba litlhare tse ncha tsa ho lekola litlhare tse ncha tsa ho lekola litlhare tse ncha tsa ho lekola litlhare tse ncha tsa ho lekola litlhare tse ncha tsa ho lekola litlhare tse ncha tsa ho lekola litlhare tse ncha tsa lefu la ho lekola litlhare tse ncha tsa lefu la ho lekola litlhare tse ncha tsa ho lekola litlhare tse ncha tsa ho lekola litlhare tse ncha. Ho feta moo, lipatlisiso li sebelisaRabbit PBMCba na le motheo oa tlhahiso ea antibody le thuto ea mathata a autoimmune, le rabbit pbmc lipatlisiso tsa bohlokoa li sa feleng. Ka ho sebelisa merafong ea Minipig pbmc le rabbit pbmc ka tandemam, bafuputsi ba ka hlahloba katleho le polokeho ea litsamaiso tsa pōli e kholo ea batho ba bangata.

Feline leCat PBMCPatlisiso e sentse niche ea eona thutong ea tšoaetso ea morao-rao le ho fokotsoa ke ho itšireletsa mafung. Patlisiso e sebelisafeline pbmcba felisitse ts'ebetso ea rona ea sesole sa 'mele oa litlamo, ha a ntse a ithuta ka katse ea PBMC ka mor'a ho itšireletsa mafung a amanang le tšoaetso ea vaerase. Ka bobeli Feline PBMC le Cat PBMC e totobatsoa khafetsa lipatlisisong lipakeng tsa maano a feline le katse ea ho itšireletsa mafung ea lefu la linoko.

Avice avanooology ha a sala morao, lePbmc ea likhohommeDuck PBMCHo fana ka lithuto tse fanang ka maikutlo a hlakileng ka lebaka la linonyana. Patlisiso e sebelisang likhoho PBMC e se e ithutile lipotso tse ikhethang tsa likarabo tsa Avian PBMC ho boetse li ile tsa thusa kutloisiso ea rona ea ho hanyetsa Pathogen of Avian. Ts'ebeliso e phetoang ea likhoho e phetoang ea likhoho e phefumolohang ea likhoho e phefumolohileng le maballo a pherekano ea tlhahlobo le katleho ea vaerate e hlalosang maloetse a amang mafu a amang mafu le baahi ba batho.
Ntle le moo, lithuto tse sebelisangGuinea pig pbmce bile Central ea Central ea Helculos le Vacione, moo pigm ea Guinea e lulang e le matšoao a tšepahalang a karabelo ea ho itšireletsa. Ka tsela e tšoanang, lipatlisiso tse amanang leAlpaca pbmcmmeKamele pbmcho hohetse tlhokomelo e ka thōko ka lebaka la meaho e ikhethang ea antibody e teng Mefuta ena. Patlisiso e amanang le Alpaca PBMC le Camel PBMC e senotsoe likopo tsa nolvel, ka alpaca PBMC e sebelisoa khafetsa ho lekola litlhahiso tsa Nanomput.

Menehelo eaBOVINE PBMMCmmeSheep PBMCHo khekhe ea khesetiki le eona e bile e tsotehang. Lithuto le Bovie Pbmc e fane ka leseli le tebileng ka maloetse a phefumoloho le lipatlisiso tse ngata tse sebelisang bolokolohi bo nang le tšusumetso e hopolellang ho ntlafatsa bophelo bo botle ba likhomo. Ka ho tšoanang, tšebeliso ea linku e phetoang ea PBMC e pheta-phello ea likotlo tsa tikoloho le ho itšireletsa mafung ea ho ruruha le lipatlisiso tsa linku tsa Pebm.

Karolo ea Boikemisetso ba Istieution

PBMC e ka etsisa tikolohong ea vivo le ho fana ka katleho e kholo ea vitro bakeng sa nts'etsopele ea lithethefatsi. Ka ho boloka litlamorao tsa lithethefatsi ho PRMC Atlame, Apoptonis le Cytotoxicity, katleho le polokeho ea lithethefatsi li ka hlahlojoa. Ka ho ithuta ka ho ithuta ka lithethefatsi ka litsela tsa ketso le litsela tsa ho bontša litsebe tsa PBMC, 'me liphetho tsena li ka fana ka maikutlo le tataiso tse ncha bakeng sa ntlafatso ea lithethefatsi.

Likopo naheng ea Antibody - Sele e ITHUTILE - Cytotoxixicity ea "cytotoxicity ea" adcc)

Ts'ebeliso ea PBMC nakong ea ho sibolla lithethefatsi ho emeloa keAntibody - Sele: Sengoli sa Cytotoxicity (ADCC). Selebotho ea antibody - Sele e tšepahalang - Sengoli se ngotsoeng Ts'ebetso e ikhethileng e ka utloisisoa ka tsela e latelang: Karolo ea fab e tlama ho opiginti ea antigenic e kenang ka seleng e lebisitsoeng. Karolo ea FC e thibetsoe ho Fru ho ea ho FX Defornin le Granzyme ba kena selekaneng ka pore e entsoeng ka seleng e lebisitsoeng ea ho shoa ka apoptosis - li-enzyme tse amanang le ho apo -

Likopo hoOthnts'etsopele ea kalafo e kholo

• - Karabelo e kopaneng ea lymphocyte (MLR)

Lisele tsa mantlha tsa DC le liseele tsa TV e le tsetsohileng tsamaisong e ka sebelisoang ho lekola ho lekola DC - Setso sa Cel Lisele tsa DC li bontša PD - L1 ka bongata bo boholo ba bona, bo tlamang ho PD - 1 holim 'a lisele tsa T ho e thibela. Haeba moriana o ka thibela tsela e atlehang tseleng pakeng tsa tse peli, e ka susumetsa ho eketsa lisele tsa T tse ngata, ka hona li netefatsa ts'ebetso e sebetsang ea moriana.

- Tlamahano

T - Assation ea selefouno e 'ngoe ea sele e' ngoe ke e 'ngoe ea likatsetso tse sebelisoang ho lekola litlamorao tsa litlhare tsa ho ikoetlisa. Likarolo tsa ts'ebetso tsa Cell

- T Cell Prolliferation Assay

T - Assages Assages Assages e ka sebetsoa ho bakang bakuli ba inmngononotherah le ho lekola katleho ea lithethefatsi ka ho bapisa sehlopha se fapaneng sa ho bapisoa le sehlopha sa taolo

- Staphylococcal enterotoxin bbmcs

Staphylococcal enterotoxin B ke ho kopanya limolek'hule ho li-molecules ho Antigen - Ho hlahisa lisele. Staphylococcal enj e na le bokhoni ba ho se be moo - ka kotloloho le lisele ebe li hokahanya ha li-cytokline tse 2, tse joalo.

PBMC karolo ho cytokine RESOL RON SYNDROME

PBMC e bapala karolo ea bohlokoa nts'etsopele eaCytokine RESOL RY SHANDRONE(CRRS), e bohloko ebile e ka ba kotsi ea ho tšoasoa ke bophelo bo kotsi bophelong. Ha a susumetsoa - hore na lisele tsa lisele tsa lisele tsa Carle le ka potlako li sebetsa ka potlako joaloka Il-6, IL Tlhahiso ena e feteletseng ea cytokine e ka baka ho senyeha ha liketsahalo ho ka etsa hore ho be le feberu, holcular boarturlar, sypotenike le setho sa sethoto. Ho utloisisa ts'ebeletso ea PBMCS ho CRS ho lebisitse kholisong ea ho kenella ka kotloloho, joalo ka sefefo sa boikhathollo sa IL-6 ka ho khutlisa lithupelo ka ho futhumatsa khatello ea maikutlo ha u ntse u boloka litlamorao tse lakatsehang.

ConKhohlano

Ka kakaretso, sengoloa sena se khothatsa karolo ea bohlokoa eo PBMCS e le nkang ho ntšetsa pele mekhabiso ea lintho tsa ho itšireletsa ka methapo e mengata ea mefuta ea Mechini ea Mechini e pharaletseng. Ka ho hlahloba menehelo e ikhethang - e leng phoofolo e kholo ea maano a morao-rao, lithuto tsena li boetse li bula tsela bakeng sa maano a sa ntlafatsang a samateperic. Khokahano ea lipatlisiso tsa PBMC ka libaka tse fapaneng tse kang ntlafatso ea li-ente, le ho hlahlojoa ha thepa ea lipale tsa lipale le tsa bongaka. Qetellong, lifapano - ho itlhahloba ka mefuta ea Pbmc ea PBMCS ho tiisa boleng ba mathata a lichelete bakeng sa mathata a mangata a kalafo.

KLinoko tsa Ey: Sele ea maeo, Cynomolgus monkey pbmc, Rhessus monkey pbmc, Ntja pbmc, Canine pbmc, Rat PBMC, Mouse PBMMC, Li-hammc, hamster pbmc, Minipig Pbmc, Rabbit pbmc, Fine PBMCC, Cat PBMC, Kuku ea Pbmc, Duck PBMCC, Guinea pig pbmc, Alpaca pbmc, Camel Pbmc, Bovine PBMC, Sheep PBMC, Antibody - Sele: Sengoli sa Cytotoxicity (ADCC), Cytokine RESOOL RY SHOCME, Frozen PBMC, CryopadedErd PBMC,Ho itšehla thajana, Thawing pbmc, Ho Seld PBMC, Leukopak, Leukaphebisos PBMC, Hlla Typed PBMC.