Tlhahlobo ea vitro ea Vitro Burro: Ho Hlahloba Likarolo tsa Abc Vesicles le lisele tsa SLC

Mantsoe a bohlokoa: ATP -, Translaer ea ABC, Mate2, Mate1, Mate1 Mock, mock slc Transruer.

Lihlahisoa tsa Iphase

Semelo

Mesebetsi ea bongaka hangata sebelisa lithethefatsi tse ngata ka nako e tšoanang, 'me lingaka tsena li ka hlahisa lithethefatsi - Lits'ebetso tsa lithethefatsi (DDI) Seo se ka ba le karabelo e mpe ea kalafo kapa ho fetola katleho ea kalafo. Hangata tlhahlobo ea DDI e qala ka tlhahlobo ea vitro ho tšoaea liphoso tsa lithethefatsi ho hlakola mekhahlelo ea DDI ea DDI le ho fumana li-parameter tsa li-KINTIC tsa ho ithuta. Ho na le metabola Metaboisang e le khale e le sehlooho sa lipatlisiso tsa DDI. Lilemong tsa morao tjena, ho nts'etsopele ha lilebeglar theknoloji ea biolocula, khatelo-pele e kholo e sebelisitsoe thutong ea vitroro, 'me bohlokoa ba ho laola libaka tse thehiloeng ho vitro bo hohetse tlhokomelo e ntseng e eketseha.

Lipapatso le likarolo tsa bona

Tsamaiso e bua ka li-spens tsa transmbrane ho pholletsa le selembrane ea lisele tse fapaneng tse kopanang le lits'oants'o tsa botsitso kapa kantle ho membistone. Liphallehareng tsa lingaka tsa vitro ke polelo e akaretsang ea liprotheine tse fetolang litlhare tse tšoanang le lisele tsa lisele, haholo-holo ho kenyeletsa malapa a mabeli: the Atp - Fumana Cassette (ABC)Superfamily le Sulute Carrier Strafamily (Lipalangoang tsa SLC), e laolang tšebetso ea lipalangoang tsa transmembrane.

Liphetoho tsa ABCNa mefuta ea mekhahlelo e itšetlehileng ka ho fetela ka cheseho e fapaneng (E.G., li-Ions, liphids le lithethefatsi) hangata ho bahloang. Ba bapala karolo ea bohlokoa ea ho hanyetsoa lithethefatsi, metabolism, le delicing ea hole.Lipapatso tsa slcHa e le hantle, ho na le basebetsi ba sa sebetseng ba sebetsang kapa ba hlokomeloang ba khothatsang motsamao oa lilallo joalo ka Glucose, Malino acid le neurotransmitters. Ho fapana le liphetoho tsa ABC, ha li hloke ATP ka ho toba empa o itšetleha ka li-ralitima tsa Ion.

Lipapatso tse khothatsang tse thusang tsa lithethefatsi tse kenyeletsang haholo ho kenyelletsa P - Glycoprotein (PO - GYCHOPROTEIN (leq: Ke litho tsa atp - Lelapa la ho palama le bona le tlamang la likaletso Lipatlisiso tse amanang le ho kena ha lithethefatsi ka seleng li ka nka thepa ea lelapa ea tharollo, haholo-holo Translantision

Lipalami tsa bohlokoa le likarolo tsa bona:

• -Bsep(Ble letsoai la kantle ho naha):Ho bohlokoa bakeng sa secretion ea bile acid ka sebete, ho senyeha ha mpa ho hokahanngoa le lefu le bileng le sebete le sebete. Sethethefatsi - ho susumetsoa -- Sikine Bsebenoition e ka baka hujotoxicity.
• -BCRP(BCRP affects the bioavailability of chemotherapeutics and antivirals, exporting substrates from cells and influencing placental and blood-brain barrier penetration.
• -Mate1 / Mate2 - K(Multidrug le proxin Experishene):E fumaneha ka li-tisse tsa Risal le hepatic, Mate1 le Mate2 - K "Lithethefatsi
• -Oatp1b1(Slco1b1):Ho tsamaisa hepatic polane e bohlokoa bakeng sa tumello ea Statin. Oaatp1b1 e bapala karolo ea bohlokoa ho thuisanang le lithethefatsi tse kang bilirubic af ho bina litlhare, lithibela-mafu le lithibela-mafu le lithibela-mafu le lithibela-mafu le lithibela-mafu le lithibela-mafu le lithibela-mafu.
• -Oat1(Slc222a6):Mecha ea Media e Tšokelo ea Chelete ea Li-Anions, ho kenyelletsa le li-antivirals le li-antivirals le tsa li-nsaids tsa li-neprostora

Ho tsamaisana le ho tsamaisana le DDI

Ho tsamaisoa ka thepake karolo ea bohlokoa ea kholo ea lithethefatsi. Ho tsamaisa thepa ho bapala ka mokhoa o nkang boima ba lithethefatsi lithethefatsing. Ho thibela lipalangoang tsa Efflux tse kang MDR1 (leq - .... Ka mokhoa o ts'oanang, ho thibela lipalangoang tse kang oatp1b1 ho ka fokotsa mokhoa oa lithethefatsi, ha BlePatic a ka kena-kenana le li-bid. Ho feta moo, lipalangoang tse kang Mate1, Mate2 - K, le oat1, e leng oa bohlokoa bakeng sa tšebeliso ea lipalesa le ho kenya letsoho ho bokellang lithethefatsi. Mefuta ea vitro, ho kenyelletsaMDKE IIMmeCaco - 2Mehala ea sele, e sebelisoa haholo ho lekola litšebelisano tsena tsa lipalangoang, ho fana ka leseli la bohlokoa ho tse ling tsa li-DDI nakong ea ts'ebetso ea lithethefatsi nakong ea lipalesa.

Mereng ea vitro bakeng sa lithuto tsa liparang-pele

Kholiseho ea Tsamaiso ea Tsamaiso ea Tsamaiso ea Tsamaiso ea Tsamaiso ea Tsamaiso ea Lipapali - Bafuputsi ba sebelisaHek293 MockLisele e le taolo e mpe, e netefatsa hore lipalangoang leha e le life tse bonoang li bakoa ke moahi oa pele. HEK293 Monka o fana ka semelo se sa fetoheng, ha hek293 Mock o lula bohareng ba ho netefatsa maemo a liteko. Mekhoa e le 'ngoe e sebelisaMesbraneHo tloha ha li halika ho tloha ho alipheto, hangata lisele tse halikiloeng ho tsoa ho lisele tsa hae293. Ka mokhoa o ts'oanang,Mock Sluc TsamaisoMefuta e bohlokoa bakeng sa ho khetholla mosebetsi o ikhethileng oa lipalangoang ho tsoa ho sethaleng se sephara. Mock Slc Baselli ba tiisa hore motsamao oo o tlamileng o fumana lipalangoang - Mokhoa ona o le mong o sebetsang o sebelisa hek293 Mock mme mock moqapi o ntlafatse bokhoni ba rona ba ho ithuta ka theko ea metaboli ea cellular.

Caco - 2 Cell Bokhoni ba eona ba ho khetholla ho enjine - joalo ka lisele tse bontšang bohlokoa haholo bakeng sa ho hlahloba ho nepahala ha lithethefatsi le ho lekola tšebelisano.

Ho feta moo, ho tsamaisa - Matsoho a Bohlokoa joalo ka lisele tsa motho li bonts'a lipatlisiso tsa subtation, le boitšoaro ba kinotic ba ho fetola tšebetso ea lipalangoang. Ha o kopantsoe le data e tsoang ho Vedicular, litsamaiso tsena tsa lisele li fana ka leseli le felletseng matlang a lipalami - phaello ea lithethefatsi.

Sephetho

Ka kakaretso, ho utloisisa litšebeletso tsa ho tsamaisa thepa ho bohlokoa bakeng sa ho ntlafatsa polokeho ea lithethefatsi le katleho. Lipapatso tse kang MDR1 (PRR1 (PCRP, LE BSPO, LE BSPO Ts'ebeliso ea mefuta ea vitro e kenyelletsang MDCY II, Caco -, Caccrain Lisele tsa II tse fanang ka litšebeletso tsa lithethefatsi le lithethefatsi ka kakaretso. Litaba tsena li bohlokoa haholo bakeng sa ho ntlafatsa ts'ebetso ea lithethefatsi le polokeho maemong a Clinical.