Microsoomes ho Metabolic thetertallic

Iphase Biosycienciens Lihlahisoa

Microsomemes ke li-vesicles tsa sucumlurlur tse tsoang ka tlung ea lisele tse silafetseng, haholo-holo lisele tsa sebete). Li na le meriana ea lithethefatsi - Ho kopanya li-cyzymes, haholo-holo The Cytochrome P450 Metabolic e botsitso ea metabolic e sebelisang Microsomes Ka ho lekanya sekhahla sa metabolism VITRO, bafuputsi ba ka hakanya tumello ea ho itšepa le ho fetelletsa lintho tseo ba li etsang ho nahana hore na sethethefatsi se ka etsa joang ho batho. Masala a joalo ha a tsamaisa pokello ea metsoako e mengata ea lipina tse phahameng - Ho thusa ka mokhoa o ts'oanang le litsebi tsa metabolic le litšebelisanong tsa lithethefatsi. Ho kopantsoe ha Microsomes ho tsoa tishu e fapaneng e lumella metabolism ea hetsatic le e bolaeang, e bohlokoa bakeng sa liteko tsa lithunya. Microsomemes tse tloaelehileng hoMetabolism e tsitsitsengAssance e kenyeletsa:Liver e sebetsanang le li-miricle tsa microsory / li-microcal, lung microsome, lung micisome.

Li-Microsomes

Li-Microsomese ruile haholo-holo li-enzchrome p450 tsa li-bozyme le litsietsi tse amanang le tsona. Likahare tsa bona tse phahameng tsa enzymatic li etsa hore li-Microsomes tse ratoang bakeng sa ho hlahloba botsitso ba metabolic. Nakong ea tlhaselo, mokhetho oa lithethefatsi o fetella ka har'a li-Microsomemes tsa sebete joaloka Nadph, 'me tekanyo eo e leng metaboselile e beiloe leihlo ka nako. Tlhahisoleseling e entsoeng ho tsoa ho liteko tsena e sebelisoa ho bala ho kenella ha kutlo, paramente ea bohlokoa e thusang ho paka kapele hore na sethethefatsi se ka felisoa kapele hakae ka vivo. Hobane Microsomes tsa sebete li ka tšosoa ho tsoa litseng tse ngata, li fana ka tsamaiso e tšepahalang le e hlahisoang e fokotsa phapang ea tlhaho ea likokoana-hloko.

Microsomes tsa Microsomes / li-Microsomes tsa mala

Micitar ea mala, ka linako tse ling e tsejoa e leMichaelo a kelello, leha e le e sa chelete e ngata ea metabolic e bapisoa le li-moimana tsa tsona tsa Hepatic, li bohlokoa ka ho lekana tikolohong ea pele - Tlosa metabolism. Kamora tsamaiso ea letsoho, lithethefatsi e tlameha ho feta leboteng le lesepa le ka ho fetisisa moo e ka tsamaeang ka mokhoa o hlophisitsoeng. Ts'ebetso ea Metabolic Microsomemes e ka susumetsa haholo puo ea lithethefatsi, 'me tlhaiso-leseling e fumanoang le litlatsetso tsena e bohlokoa pele - Sisdom Metabolism.

Li-microsomes tsa letlalo

Letlalo Microsomemesli lokiselitsoe ho tsoa ho lisele tsa letlalo 'me li bonts'a sethethefatsi - LITLHAKISO TSA MOTSOALLE OONE, ho kenyelletsa le tsa li-enzyme tsa cons con. Ha ketsahalo e itseng ea enzymatic lethekeng ka tloaelo e ka tlase ho 10% e fumanoang sebete, letlalo le phetha karolo ea bohlokoa ho binonobioticsfsform ea lijo tsa teng. Ho sebelisa melatsoana ea letlalo ho assays ho ka fana ka leseli la metabolism ea methapo e sebelisoang haholo kapa e kenella letlalong.

LungMicrosomemes

Mathata a microsomemesli lokiselitsoe ho phofo 'me li sebelisetsoa ho etsa lipatlisiso ka methapo e tsamaisoang ka ho inhalation kapa e nang le litlamorao tsa tsona ka lehlakoreng la phefumoloho. Ha le mahloriso a li-enzyme tsa cytocrome p450 ka matšoafong, lung e lula e le sebaka sa bohlokoa bakeng sa metabolism ea chefo ea tikoloho le meriana e khaotsoeng. Moetso ona o bohlokoa haholo ho lekola lithane - Litlhare tse ikhethang tsa metabolication le chefo ea lehae.

Liphio Microsomemes

Liphio Microsomemesli qheletsoe thoko ho lisele tsa renal 'me li fana ka leseli lits'ebetsong tse etsahalang mericalic tse etsahalang monoana. Kaha letsibolo ha se setho sa thabo feela empa hape e kenya letsoho ho hloekisitsoeng ka metabolication, tšebeliso ea Microney Microssors e ka lekola sebopeho sa metabosonome se ka amanang le metabosonoxicity. Ka tsela ena, li-Microsomes tsa liphiotsoana li tlatsa data ho tsoa ka sebete le ka mpeng, ho fana ka pono e pharalletseng mabapi le profinseng ea metabora ea metabolic.

Testis Microsomemes

Testis Microsomemesli tsoa ho lisele tsa temoso 'me li na le li-enzyme tse ikarabellang bakeng sa metsoako ea melisa e hlophisitsoeng le ea Exogenus. Le ha e se e sebelisoa hangata ho feta Microsomes tsa sebete, li ka sebetsana le ho ithuta lintho tse amang bophelo ba ho boloka bolisana. Lintlha tse ikhethang tse sebelisoang litsing tsa botsitso tsa metabolic li na le meeli ebile li ka fapana ho latela tsepamiseng ea lipatlisiso.

Midididymis Microsomemes

Epididymis Microsomemesli fumanoa ho tsoa ho lisele tsa sephakeng, 'me, joalo ka Testis Microsoomes, e kenya letsoho methani ea metsoako e itseng. Ts'ebetso ea bona ho Metabolic e khutsitseng ea metabolic e atile haholo, empa e kanna ea sebelisoa lithutong tse hlahlobang lintho tse bakang metabole le tšebetso ea ho etsa. Li-protocols tse qaqileng le tšebeliso li ne li tla itšetleha ka sepheo se itseng sa lipatlisiso.

Phase I Metabolism & Nadph Regneration Systeen

Phase I Metabolic Karabeloli tsamaisoa haholo ka linezyme tsa pompo, 'me maikutlo ana a hloka ho fokotsa boima ka mokhoa oa Nadph. Ho etsa bonnete ba hore Nadph e fumaneha ho pholletsa le nako ea poloko, aSistimi ea Nadph ea Nadphe eketsoa ho assay. TheSistimi ea Nadph ea NadphHangata ho kenyelletsa Nadp⁺, Glucose - 6 - fracose, le glucose ea enzyme - 6 - Copthote Dehhhydrop Moqapi ona oa bohlokoa o hlokahala hobane o boloka liphetoho tse redox tse fokolang ke li-enzyme tsa cyp, li lumella Microsoomes hore li lule li le teng ka linako tse telele.

Feiga 1. Karol ke karabelo tseleng ea lithethefatsi
Phase II Metabolism & Ugn Incubation System

Ha li-microsomes tsa sebete li amana haholo leKarol i metabolism, li ka tloaela ho ithutaPhase Rectionjoalo ka glucusidation. Glucuronidation ke ts'ebetso e kopaneng ea karolo ea bosholu ba urjugline 5 '- Diphospho - Ho thusa bokhabiso bo sa sebetseng ho assance microval, aSistimi ea Uggka cofactor e etselitsoeng udp - sebapali sa acid (Udpga) e eketsoa. Sisteme ea ho inkela e sa sebeliseng hangata e na le udpga, protheine ea procymidin le D - Glusulanosyl - 1.4 - lacattone. Kaha li-enzyme tsa hau ke membrane - li tlangoa ebile li ka ba tse saloang bonyenyaneng bo sa tsitsang, e leng pore - ka linako tse ling e babaloa ka linako tse ling e leng alamethic. Alamethicin e eketsa ho nepahala ha membrane membranes, ka tsela eo, ho ntlafatsa phihlello ea Uddga ho ea sekhukhu le ho ntlafatsa ts'ebetso ea karabelo ea khalemelo ea khale.

Sagy 2. Liphetho le lihlahisoa tse iqapetsoeng tsa phaoboli ea lithethefatsi.

Sistimi ea Buffer

Nakong eohle ea ts'ebetso eohle, the0.1m pbsBuffera e bapala karolo ea bohlokoa ho boloka botsitso le tšebetso ea enzyme. Sistimi ena ea buffer e fana ka ph e fana ka ph le tikoloho e sa lebaleheng, e bohlokoa bakeng sa ho boloka botšepehi bo hlophisitsoeng ba li-enzyme tse sa sebetseng. Maemo a sa fetoheng a theko e anngoeng ke 0,1m PB e netefatsa hore boits'oaro bo etsahala ka mokhoa o laoloang, ho nolofatsa tekanyo e tšepahalang ea botsitso ba metabolic le tumello.

Mefuta e fapa-fapaneng ea Microsomemes

Anical

Microsomemes ea bathoA na ka ngangisana ka ho fetisisa ho meholo ka botsitso ba metabolic bakeng sa nts'etsopele ea lithethefatsi, kaha ba ntse ba etsisa tikoloho ea metaboli ea maoatle a sebete. Li-Microssome tsa batho li na le khatello e phahameng ea Cytochrome P450 Enzmes, e leng boikarabello ba karolo eo ke e ratang haholo. Li-Microsomes tsena li sebelisetsoa ho lekola metabolism ea lithethefatsi, ho kenyelletsa le enzyme - Bokhabane ba Metabooli ba nang le chefo. Tšebeliso ea bona ke ea bohlokoahali - Motsoako oa lithethefatsi ho netefatsa hore komporo e tla ba le li-pharboke tse ntle ho batho, ka leihlo le leng le bohloko.

Eseng - Li-Microsotes tsa Li-Microsomes

Eseng - Microsomes ea Boforami ea Bofetisi, hangataRithes Monkey Liver Microsomes, Marmosets Monkey Livers Microsomes, kapa litšoene tsa cynomolgusLi-Microsomemes, li sebelisoa ho Metabolism e tsitsitseng ho lekola hore na mekhamphani e metaeli joang ka har'a sebete. Li-Microsme tsena li na le li-enzchrome p450 tsa li-profinse tsa metaboli tsa metaboli tsa metabolication tse tsamaisang karolo ea ho sebelisa metabolism. Batho bao e seng mocha oa batho ba bohlokoa haholo lithutong tsa batho ba khethang ka hloko, 'me ba ka ba le botsitso ba metjeko ea metricialli. Li fana ka lintlha tse loketseng ho batho - joalo ka metabolism bapisoa le litoeba, ho ntlafatsa lisebelisoa tsa ntlafatso ea lithethefatsi

Li-Koloi ea ntja

Lintja, haholo-holo lintja tse hlaba, li sebelisoa hangata lithutong tsa maano le pharmakokotic.Li-Microsomes tsa ntja, Haholo-holo ba fuoeng le sebete, ke lisebelisoa tsa bohlokoa tsa ho utloisisa hore na lithethefatsi li ka ba le metaeling joang ho a phoofolo e anyesang e anyesang.Minine li-MicrosomemesHangata ba sebetsa tlhahlobo ea polokeho ea pelehi ho lekola botsitso le bokhoni ba lithethefatsi - Tšebelisano ea lithethefatsi. Li-Microsomemes tsena li ka boela tsa thusa ho bolela esale pele hore na lingaka li tla kena joang 'me li sebelisoe ho batho le lintja mabapi le metabolism ea lithethefatsi.

Rat Liver

Likhoto ke e 'ngoe ea liphoofolo tsa laboratoring e sebelisoang haholo ho feta tsa meriana le lipatlisiso tsa chefo,' me li-microsome tsa tsona tsa sebete li bohlokoa ho metaboelic.Rat Liver Liver Microsomesli sebelisoa hangata mathoasong a pele - nts'etsopele ea lithethefatsi ho lekola metabolism ea likhamphani tsa liteko, joalo ka lits'ebetso tsa tsona tsa metaboli li hantle - ho utloisisoa. Le ha likhoto li arolelana tsela e 'maloa ea metaboli e' maloa le batho, ho na le phapang ea mosebetsi oa enzchrome, haholo-holo ka tlhompho ea li-cytochrome p450 enzyme. Li-Microsomemes li sebelisoa khafetsa ho leka botsitso ba metabolic le ho lekola litaba tse ka bang phapanyetsano, joalo ka litekanyetso tsa leano le bigagalical.

Mouse sebete ea mouse

Ho tšoana le likhoto, litoeba li sebelisoa haholo lipatlisisong tsa biomedical, le meuse Microsomes li bapala karolo ea bohlokoa ho metaboelic e tsitsitseng. Lito tsa litoeba ke tsa bohlokoa ka ho khetheha bakeng sa ho ithuta liphapang tsa liphatsa tsa lijo tsa lefutso metabolism ka lebaka la phepelo ea tsona - mofuta o tsebahalang -Li-Microsomes tsa Mouse liverE na le mefuta e mengata ea li-conzchrome p450times, e etsang hore e etse molemo bakeng sa ho hlahloba hore na lithethefatsi li ka 'na tsa tšeloa ke lithethefatsi joang. Mefuta e itseng ea litoeba, balb / C nude, e tsejoa e le khatello ea thym, e etsa hore li senyehe. Ka ho sebelisaBalb / C Nude Liver Microsomes, bafuputsi ba ka hlahloba hore na sethethefatsi kapa sethethefatsi sa eona se na le eng, 'me ke ho se sebetse hantle ho batho, ho bolela esale pele ho feta armacokinetic ho batho. Leha ho le joalo, li-Mice li na le mekhoa e ikhethang ea metabolic ho ba le batho, ho bolelang hore data e tsoang ho mouse Microsomes e lokela ho hlalosoa ka hloko ha motho a e-na le tlhokomelo ea batho. Li-Microsomes tsa mouse li sebelisoa ho phahameng - ho kenya tlhahlobo ea ho lekola palo e kholo ea metsoako kapele.

Hamster sebete ea hamster

Li-Hamsters, haholo-holo Syanters tsa Sybianters, hangata li sebelisoa lithutong tsa metabolic ka lebaka la litšobotsi tsa tsona tse ikhethang tsa 'mele.Hamster Liver Microsomesli thusa ho lekola metabolism le toxicology, haholoholo bakeng sa metsoako e ka bonts'a mefuta e ikhethang - metabolic profiles. Hangata Microsomemes hangata e sebelisoa ho ithuta hore na lifate li na le liphoofolo tse anyesang tse manyane, li fana ka leseli litseleng tsa metaboli li ka 'na tsa se utloisisoa ka botlalo memelong e meng ea metabono.

Gerbillinae Liver Microsomes

Gerbillinae Liver Microsomesli tsoa ho li-balebeli, setho se anyesang se anyesang hangata se sebelisoang hangata ho chemisi ea mafficology le ParmaCology. Ho Metabolism the botsisi ba Gerbilline ba sebetsang ho lekola hore na motsoako o joang ka har'a li-enzyminate tse tlang nakong ea li-enzysal tsa microctosal. Likarolo tsena li thusa ho khetholoha ha lithethefatsi kapa lik'hemik'hale, ho lekola monyetla oa bokhoni ba bilione le ho felisoa. Ka linako tse ling li-gerbille ka linako tse ling li sebelisoa bakeng sa lithuto tsena ka lebaka la profil ea 'mino e tona ea metabolic, e ka fanang ka ho utloisisa mefuta ea Metabolic, e ka fanoang ke mefuta -

Minipig Liver e Miniphor

Li-mnipigs li ntse li eketseha ho ba tummeng ho ea pharmaconeetic le lithuto tsa maxicake tsa ho phela ka tsela e tšoanang le ea tsona, haholo-holo ho batho, haholo-holo ho latela sebete ho ea metabolism.Micropig Liver MicrosomemesHangata li sebelisoa botsitso ba metabolic ho fana ka datha e tšoanang le metabolism ea lithethefatsi e bapisoang le metabolism ea lithethefatsi e bapisoang le metabolism ea lithethefatsi. Li-Microsomem tsena li na le thuso e ngata bakeng sa ho ithuta ho atjha, ho tsamaisoa, metabolism le phepelo (adme) ho motho ea mongata - joalo ka boemo ba metabolic. Li-mnipigs li bohlokoa haholo ho ho hlahloba lijalo ho lekanya metsoako e nepahetseng haholoanyane ea likarabo tsa batho tsa batho ba metabolic.

Lithuia Cover Microsomes

Ho fapana le litorone tse ling, likolobe tsa Guinea li haella ho sebelisa lithethefatsi - metabosome p4chrophe, e kang Cytochrome P450 2D, e ka ama tsela eo ba sebetsang ka eona ka eona. Sena sea etsaGuinea Cob MicrosomesHaholo-holo thuso bakeng sa ho ithuta Mefuta - liphapang tse khethehileng tsa metabolism ea lithethefatsi. Boemo ba bona bo ikhethileng ba enzyme bo ka fana ka leseli la hore na motsoako o ka 'nang oa itšoara joang meabolawaws kapa kotsi ea metabolism ea lithethefatsi e ka' na ea se ke ea bonoa melimong e meng. Sena se etsa likolobe tsa Guinea tsa bohlokoa bakeng sa likhakanyo tse bapisang le tsa phaello le pharmaconetic.

Fealine Liver e Micro Hand

Fealine Liver Microsomesli sebelisoa lithutong tsa metabolic ho lekola hore na lithethefatsi li sebetsoa joang likatse. Likatse li na le litšobotsi tse ikhethang tse ikhethang, ho kopanyelletsa le ketsahalo e fokolang ea bokhabiso e fokolang, e ka amang metabolism ea lithethefatsi tse itseng. Ka lebaka la sena,Katse Li-Microsomes tsa Catli bohlokoa bakeng sa ho ithuta hore na mekhahlelo e ka sehloohong e itshware ka maiketsetsong Li sebelisoa ho lekoa ho batho ba ka bang le chefo kapa metabolic ka litlhare tsa metabolic ka litlhare tse etselitsoeng tšebeliso ea methapo ea maikutlo ha ho fetoleloa ho tloha ho batho ho ea liphoofolo lithutong.

BoVine Lint a Microline

Bovine sebete tsa li-microsomes, e tsoang likhomo, e tsoa ho likhomo tse ngata tsa ho ithuta metabolism e sebelisoang mehlape. Likhomo li na le litselana tse fapaneng tsa metabolic ho bapisoa le batho, haholo-holo mesebetsing ea bajari ba sa sebetseng ba amehang ho60 ho metabolism. Li-Microline Liver Microsomes li sebelisoa ho bolela esale pele hore na lithethefatsi tsa matloana kapa lik'hemik'hale tsa temo li tla ba metaelisitsoe ka likhomo. Ho feta moo, Microsomemes ena e sebelisoa ho etsa lipatlisiso tsa masala a bonolo le lebese, ho thusa ho etsa bonnete ba hore polokeho ea lijo bakeng sa tšebeliso ea batho. Le ha li-Minemine Microsomes li fana ka data ea bohlokoa metabolism, e kanna ea se sebetse ka kotloloho ntlafatso ea lithethefatsi tsa lithethefatsi tsa lithethefatsi ka lebaka la liphapang tsa lithethefatsi. Kenyelletsa ho bovine,ho lipere tsa pere,Li-Microsomes tsa linku tsa sebeteMmeLi-Microsomes tsa polili sebelisoa hampe.

Li-Liver tsa Lipothorry li sebetsa

Microsomemes, ha a ntse a sebelisoa hangata ho feta tseo ho tsoa lithung tsa liphoofolo tse atileng, li ka ba molemo lithutong tsa metabolism ea lithethefatsi. Li-microsomes tse tloaelehileng tsa likhoho li kenyelletsaMicrosomes ea sebete,Li-Microsomes tsa Kuku,Turkey Liver MicrosomesMmeLi-Microsomes tsa Quail Liver. ba hiriloe ho hlahloba hore na lithethefatsi li ka sebetsoa joang ho mefuta ea Avian. Sena ke sa bohlokoa ka ho khethehileng nts'etsopele ea lithethefatsi tsa bongaka ba liphoofolo tse phelang bakeng sa liphuputso tsa tikoloho ho utloisisa metabolism ea lik'hemik'hale tse ka kenang ho linonyana.

Li-shaper tsa lihlapi tse ntle

Li-Microsomes tsa lihlapi tsa sebete, haholo-holo rakhoeboLi-Microsomes tsa Trout Liver, e hiriloe lipatlisisong tsa tikoloho le ea che. Litlhapi li nahanela haholo libaka tsa tikoloho le li-Microsomes tsa tsona tsa sebete ke lisebelisoa tsa bohlokoa ho ithuta tsela ea metabostems. Microsomemes e boetse e sebelisetsoa ho ithuta tšusumetso ea tikoloho ea mekhatlo le lik'hemik'hale tsa liindasteri, tsa thusa ho lekola bophelo ba bona le ho ama bophelo ba metsing. 

Sephetho

Mectimes tsa ho bapala karolo ea bohlokoa methating ea pele ea nts'etsopele ea lithethefatsi ka ho fana ka leseli le tsitsitseng metabolic le meriana ea bakhethoa ba lithethefatsi. Ka tšebeliso ea litsebi tsa minosal, bafuputsi ba ka hlahloba karolo ea bona le tsompho II ea II ea II, ba ka ba lithethefatsi - Metabolism e tobileng. Ho fumaneha ha Li-Microsomes ho tsoa mefuta ea mefuta e mengata ea mefuta le liphoofolo, e nolofalletsa pokello ea metaboliriso ho batho le ho netefatsa polokeho le katleho ea metsoako e mecha ea lithethefatsi. Ha ts'ebetso ea ntlafatso ea lithethefatsi e ntse e tsoela pele ho feto-fetoha, mefuta ea microstemal e tla lula e le sesebelisoa sa ho hahamalla meriana le meriana e sebetsang e atlehileng.

Mantsoe a bohlokoa: botsitso ba metabolis Pbs, TRIS - HCK, Microsomemes, CynomolGus Monkey Livers, Marmos Livers, Microsos Li-Microsomes tsa Hams tsa Li-Microsos Liver Ha e na le likokoana-hloko, li-Microsomes tsa linku tsa sebete, poli tsa sebete tsa pōli, Li-Microsomes tsa Quail Liver
Resort
O 2 Carrier e hlophiselitsoeng e le 'ngoe ea 2 Tsamaiso ea biopatic ea hepatic hollow - Sebopeho sa saense ho Patlisiso. E fumaneha ho:PETSTPS //7/figure.Net/figure/phase Mpphase
Ho hlakola karabelo ea metabolao ea Ibuprofen le matlo a mang a nang le protheine ea batho ba batho ba motho ea Plasma - Sebopeho sa saense ho Patlisiso. E fumaneha ho tsoa ho: Firps://www.resesachgate.net/figure/Frograph>