Iphaa soltion maka Salna ọgwụ na vitro Metabolism Nchoputa
Ọgwụ acid nucleic acid, n'ihi njirimara teknụzụ pụrụ iche ha pụrụ iche, abụrụla ihe a na-elekwasị anya ọgwụ ọgwụ ọhụụ n'afọ ndị na-adịbeghị anya. Ọgwụ mgbochi acid gụnyere obere ihe na-egbochi rna (Oligonucleotitiotide (AONA), pere mpe), na Aptamers RNA (ADC), bụ ụdị ọgwụgwọ nke mkpụrụ ndụ. N'ime ihe ndị a, ọgwụ Salna, dị ka hotspot na nyocha ọgwụ Nucleic acid na mmepe Nucleic acidii na-emepụta ihe niile n'ihi arụmọrụ zuru oke, akụkụ ndị na-achịkwa ya, na njikọta dị mfe. A na-atụ anya ka ha bụrụ ọgwụ ndị kacha na-adịghị mma maka mmepe ọgwụ ọhụrụ mgbe obere molekule ọgwụ ọgwụ ọgwụ.
- 1.
Obere ihe na-egbochi RNA (SERNA), na-ezo aka na RNA, ma ọ bụ na-enweghị isi nke RNA, na-enwe obere okpukpu abụọ - Na njikọ, Sirna batara sel site na endocytosis. Nkwụsị nke Sirna na-agbanarị mbibi LysSomal ma banye Cytoplasm, ebe a na-etinye ya na RNA - N'ime Risc, Sarna na-amaghị ihe abụọ a na-anọghị nha abụọ: eriri afọ na ụkọ. A na-arụ ọrụ na-enweghị uche na Cytoplasm ngwa ngwa na Cytoplasm, ebe a ga-arụ ọrụ nrịba ama, na-arụ ọrụ. Ihe mgbagwoju anya ahu bu nke oma na arna lekwasịrị anya, na-eme ka njigide ya na mmebi nke ya. N'ihi nke Mrna mebiri emebi, ngosipụta nke mkpụrụ ndụ mkpụrụ ndụ ihe ọmụmụ a na-ebelata, n'ikpeazụ na-eduga n'ọzọ Genecking na mgbochi nke ntụgharị protein.
- 2. Sirna ọgwụ nyocha Metabolism
Na Vivo, ọgwụ ọgwụ sirna bụ isi metabolized site nuclerized site na nuclerized site na plasma na anụ ahụ, kama n'oge na - emejupụta na imeju. Mgbe ị na-eso mgbanwe usoro nhazi, ọgwụ Salna ugbu a na-egosipụta belata metabolism na ọbara. Otu imeju na imeju na-ewere ngwa ngwa site na imeju, ya na obere ihe kesara na akwara ndị ọzọ, ebe ha na-esochibido ya site na nsị ndị ọzọ. Maka ọmụmụ ọgwụ Sima metabolism nke VIVO metabolically amatala na plasma, mmamịrị, feves, na imeju) site na ụdị anụmanụ.
Agbanyeghị, n'oge mmalite nke ọgwụ ọgwụ, ọnụ ọgụgụ buru ibu nke ogige, na-enwekarị usoro nyocha, yana ọnụ ahịa dị elu na-eme ihe ịma aka dị mkpa - Ntụle usoro nlele na nhazi. N'ihi ya, ọmụmụ Vitosta Metabolism bụ ihe dị mkpa n'oge mmalite nyocha nke ihe nkiri sirna. Ọmụmụ ihe ndị a na-enye uru mara mma, dị ka site na nrụgide dị elu na nke dị mkpụmkpụ, nke nwere ike ịkwalite arụmọrụ nke nyocha nke Siker ọgwụ Sike.
Tebụl 1: Na Vito Metabolism nyocha maka Salna ọgwụ
| Uzo | Akwukwo icho oru |
| Ọbara / plasma |
Na-ebute ọnọdụ ọgwụ nke metabolic nke akwara n'ọbara na n'ime sistemụ. Nke a bụ nnwale chọrọ maka na ọmụmụ ọgwụ ọgwụ Citro Sirna. |
| Liner S9 | Nwere ọtụtụ enzymes dị na anụ ahụ imeju ma na-adị mfe ịnweta. Ruo n'ókè ụfọdụ, enwere ike iji ya dị ka ihe dochie anya anụ ahụ na-emeju. |
| Anụ ahụ n'ime imeju | Usoro enzyme na-ezuwanye elu ma na-atụ aro maka vitro na-enyocha ya na ntule nke ọgwụ sirna. |
| Hepatocytes | Sistemụ Enzyme bụ ihe zuru oke, na-eme ya dabara adaba maka nyocha metaboclic nke imeju - Ileghara ọgwụ nzuzu. |
| Lysisomes | Lysisomes bụ gburugburu ebe obibi bụ isi na Sirna Ọgwụ na-ezute na mkpụrụ ndụ site na endocytosis. Ha nwere usoro enzyme bara ụba nke enzyme, gụnyere nucalases na hydroles na hydroles dị iche iche, ma na saịtị dị mkpa maka Syna Mitabolism. Lysisomes na-enye usoro nnwale zuru oke maka ịmụ ihe ndị metabolic steabilic nke ọgwụ sija. |
- 3. Iphase dị mkpa
Ndị òtù ọ bụla metụtara na mmepụta ngwaahịa na-esi isi site na ụzọ channelụ, ya na mmalite doro anya.
Nzerendo
Ma ndị ọrụ na-arụ ọrụ na ụmụ anụmanụ na-aga n'ihu na-anwale ọrịa na-anwale iji hụ na ngwaahịa na nchekwa ngwaahịa.
Ịdị ọcha dị elu
Mkpụrụ obi ọcha nwere ike iru 90%.
Oke viability
Viablity Viablity nwere ike iru 85%, nzukọ ndị ahịa chọrọ.
Ọnụego mgbake dị elu
Ọnụ ego a na-agbagha agbagha nwere ike gafere 90%.
Gbasara nhazi
Ọrụ ndị dị iche iche dị dabere na ndị ahịa chọrọ, na-enye ụdị ụdị pụrụ iche ma ọ bụ nhazi anụ ahụ.
| Edemede | Ngwaahịa iphase |
| Ihe ndị dị n'okpuru | Liver Loursomes |
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| Isi na-enweghị atụ | Nkwusioru Hepatocytes |
| Uwe dị larịtocytes | |
| Plasma | Plasma kwụsiri ike |
| Plasma protein |
Oge post: 2024 - 12 - 20 13:08:46