Principle
IPHASE's PBMC isolation kits are based on the classical density gradient centrifugation method, which is based on the difference in settling speeds of different blood cells in a density gradient medium to achieve cell separation. Included in the kit is a specially formulated density gradient separation solution that creates a stable density gradient during centrifugation. When an anticoagulated whole blood sample is slowly added over the separating solution, the denser cells such as red blood cells and granulocytes settle to the bottom of the centrifuge tube, while the less dense PBMCs are suspended at the interface between the separating solution and the plasma, forming a clear, white, cloudy layer of cells. By carefully collecting this layer of cells and subsequent washing and centrifugation, high purity PBMC can be obtained.Brief introduction
IPHASE's PBMC Isolation Kit is a tool designed for researchers to efficiently isolate PBMC from anticoagulated whole blood. The kit has the following features:1.Highly efficient separation: the use of an optimized density gradient separator ensures rapid and accurate separation of PBMC during centrifugation, while reducing contamination from other cell types.
2.High purity: through the fine separation process and subsequent washing steps, high purity PBMC can be obtained, providing a reliable basis for subsequent cell culture, molecular biology experiments and so on.
3.Sterility and low endotoxin: the isolation solution and other components in the kit have been subjected to strict aseptic treatment and extremely low endotoxin levels, ensuring that no unwanted contaminants will be introduced during the isolation process.
4.Widely applicable: not only suitable for human PBMC isolation, but also provides special PBMC isolation kits for other animal species (such as monkey, dog, rat, mouse, etc.) to meet different research needs.
5.Easy to operate: The kit is equipped with detailed operating instructions and necessary auxiliary tools, which makes the whole isolation process simple and easy, even for beginners to quickly get started.
Application examples
IPHASE's PBMC isolation kits have been widely used in various biomedical research. For example, in immunology research, researchers can use the separated PBMC for the sorting and functional study of T cells, B cells and other immune cells; in oncology research, PBMC can be used to assess the immune status of tumor patients and screen anti-tumor immune cells. In addition, the kit is widely used in vaccine development, drug screening and other fields.Conclusion
IPHASE's PBMC isolation kit has occupied an important position in the field of biomedical research with its excellent performance and wide application value. With the continuous progress of science and technology and the increasing demand for research, IPHASE will continue to devote itself to the development of more efficient, pure and easy-to-use biological reagent products to provide more powerful support for researchers. Post time: 2024-07-27 16:30:36