Keywords: Hepatocytes, Primary Hepatocytes, Plateable Hepatocytes, Suspension Hepatocytes, Hepatocytes Metabolic Stability, Hepatotoxicity, CYP450 Enzyme Induction, Primary Human Hepatocytes(PHH), Primary Cynomolgus Monkey Hepatocytes(PCH) , Animal Hepatocytes Hepatocytes, Plate Human Hepatocytes Hepatocytes , Plateable Rhesus Monkey Hepatocytes, Plateable Dog Hepatocytes, Plateable Beagle Hepatocytes, Plateable Sprague-Dawley Rat Hepatocytes, Plateable ICR/CD-1 Mouse Hepatocytes, Plateable C57BL/6 Mouse Hepatocytes, Plateable Plateable Hepatocytes, Plateable Hepatocytes Syrian Minipig Hepatocytes, Plateable New Zealand White Rabbit Hepatocytes, Plateable Chicken Hepatocytes, Suspension Human Hepatocytes, Suspension Cynomolgus Monkey Hepatocytes, Suspension Rhesus Monkey Hepatocytes, Suspension Dog Hepatocytes, Suspension Beagle Hepatocytes Hepatocytes Hepatocytes Hepatocytes Wistar Rat Hepatocytes,Suspension Wistar Han Rat Hepatocytes,Suspension ICR/CD-1 Mouse Hepatocytes, Suspension C57BL/6 Mouse Hepatocytes, Suspension Golden Syrian Hamster Hepatocytes, Suspension Feline Hepatocytes, Suspension Minipig Hepatocytes, Suspension Hepatocytes Hepatocytes New Zealand White RatASGPR, GalNAC-siRNA, Primary Hepatocyte siRNA Transfection siRNA Galnac Hepatocyte Delivery,Hepatic Targeted Delivery.
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Product Name |
Tsanangudzo |
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Kumiswa Primary Hepatocytes |
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4-6 miriyoni |
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5 miriyoni |
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5 miriyoni |
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5 miriyoni |
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5 miriyoni |
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IPHASE Suspension Rat(Wistar) Hepatocytes,Murume |
5 miriyoni |
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IPHASE Suspension Rat(Wistar Han) Hepatocytes,Murume |
5 miriyoni |
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5 miriyoni |
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5 miriyoni |
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5 miriyoni |
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5 miriyoni |
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IPHASE Suspension Rabbit(New Zealand White) Hepatocytes,Murume |
5 miriyoni |
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2 miriyoni |
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Plateable Primary Hepatocytes |
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4-6 miriyoni |
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4-6 miriyoni |
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5 miriyoni |
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5 miriyoni |
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5 miriyoni |
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5 miriyoni |
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5 miriyoni |
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5 miriyoni |
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5 miriyoni |
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5 miriyoni |
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IPHASE Plateable Rabbit(New Zealand White) Hepatocytes,Murume |
5 miriyoni |
| IPHASE Plateable Rabbit(Ichena YeJapan) Hepatocytes,Murume | 5 miriyoni |
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Accessaries |
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50mL |
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40mL |
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10mL |
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10mL |
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20mL |
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50mL |
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96Matsime/1blocks |
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48Zvitsime/1blocks |
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24Matsime/1blocks |
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12Matsime/5Plates |
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IPHASE Collagen Coated Plate,6 matsime |
6Matsime/1blocks |
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100 maitiro |
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IPHASE Primary Hepatocytes Isolation Kit |
1Set |
Cherechedza: The Suspension Hepatocytes inoda kushandiswa neThaw Medium uye Incubation Medium; Iyo Plateable Hepatocytes inoda kushandiswa neThaw Medium, Plateable Medium, Maintenance Medium, uye Collagen Coated Plate. CYP induction inoda mRNA Induction Assay Kit futi.
Hepatocytes
Hepatocytesndiwo maseru ekutanga anoshanda echiropa, achitamba chikamu chepakati mumetabolism, detoxification, kugadzirwa kwebile, uye protein synthesis. Ivo vanoumba hwaro hwechimiro uye chebiochemical chechiropa chinoshanda, vachiita sevamiriri vakuru mukushandurwa kweakasiyana endogenous uye exogenous makomisheni kuita zvinhu zvisingakuvadzi uye kufambisa metabolism yezvinodhaka uye chepfu. Uku kugona kwemukati kwehepatocytes kugadzirisa makemikari kwaita kuti zvikoshese mune zvese zvekiriniki uye zvekutsvagisa marongero, kunyanya kana uchiongorora nzira dzinobatanidzwa mumishonga metabolism uye chiropa chepfu.
Primary Hepatocytes
Primary hepatocytes inoreva iwo masero anga achangobva kuparadzaniswa kubva pachiropa tishu-angave munhu kana mhuka-asina kurebesa in vitro kupararira. Nekuda kwekuti maseru aya anochengeta akawanda ekuita enzymatic uye physiological mabasa ari pachiropa, anowanzoonekwa seyegoridhe mwero muzvidzidzo zvinoongorora chiropa-chaiwo zviitiko. Kushandisa kwavo mukutsvagisa kwakakosha pakunzwisisa zvese kugadzikana kwemetabolism uye kugona kwemukati kwemishonga kuti ishanduke kana kuramba iri muchimiro chayo chepakutanga kana yaunzwa mubiological system.
Plateable Hepatocytes uye Suspension Hepatocytes
Mukati memaitiro ekuedza, hepatocytes inogona kuverengerwa zvakare zvichienderana nemamiriro avo ekurima. Plateable hepatocytes ndeaya anoomerera kune tsika substrate, iyo inobvumira kune yakagadzikana yakabatana uye morphology inonyatso tevedzera iyo in vivo nharaunda. Ichi chinongedzo hachingotsigire chete polarized chimiro chemasero aya asiwo chinotsigira kwenguva refu - zvidzidzo zve biochemical nzira senge.CYP450 enzyme induction. Sezvineiwo, kumiswa hepatocytes inochengeterwa mune isiri - inoteedzera nyika, inowanzoshandiswa munguva pfupi - bvunzo dzenguva apo shanduko munharaunda ye cellular uye nekukurumidza metabolic kuongororwa kunodiwa. Tsika dzekusimudzira dzinofambisa kuratidzwa kwakafanana kwemaseru kuti aedze makomisheni, zvichiita kuti ave akakodzera kuongororwa nekukurumidza kwekugadzikana kwemetabolism uye acute hepatotoxic mhinduro.
Plateable Hepatocytes
Hepatocytes inoputikandiwo masero echiropa akasarudzika akagadzirirwa pandiro dzakaputirwa kana dzakashongedzerwa tsika. Ichi chinonamirwa chinobvumira maseru kupararira, kupora, uye kumisikidza intercellular contacts, kuchengetedza akawanda mu vivo - senge mabasa kwemazuva akati wandei kana mavhiki. Nekuti maseru aya anoomerera, anogona kushandiswa kutarisa mhinduro dzisingaperi, CYP450 enzyme induction nekufamba kwenguva, uye yakawanda-yemazuva toxicity assays. Plateable hepatocytes inochengetedzawo yakasimba sero - ku-sero kutaurirana uye extracellular matrix kudyidzana, izvo zvakakosha pakuchengetedza chiropa - chaiwo mashandiro. Mhuka yega yega — angave munhu, asiri - primate yemunhu, makonzo, kana zvimwe — dzingada akakwenenzverwa mamiriro ekupfeka (seakajaidzwa chaiwo kana midhiya yekuwedzera) kuti inyatso kutevedzera mamiriro emuviri.
Kumiswa kweHepatocytes
Mukusiyana,Kumiswa kwehepatocytesvanodzidziswa mune isiri - inoteedzera mamiriro, kuchengetedza yakatenderedza morphology ichiramba ichigoneka kwenguva pfupi. Kumiswa hepatocytes kwakanakira kwenguva pfupi - kuyedza, sekutanga zvinodhaka metabolism zvidzidzo kana kukurumidza enzyme kinetics evaluations. Sezvo maseru asina kumanikidzwa nematrix akabatanidzwa, anogona kukurumidza kugadzirira uye kushandiswa pakuita bvunzo pasina kunonoka kunodiwa pakuteera sero. Kumisa tsika kunonyanya kubatsira kana tichienzanisa kusiyana kwemarudzi, sezvo ivo vachibvumira yakamisikidzwa kukurumidza assay mhiri Suspension hepatocytes kubva kuvanhu, asiri -
Metabolic kugadzikana uye Hepatotoxicity
Metabolic kugadzikanamuchirevo chekutsvaga kwehepatocyte inoreva kugona kwemorekuru, kazhinji mumiriri wezvinodhaka, kuramba shanduko yebiochemical nema enzymes echiropa. Kuongorora kugadzikana kwemetabolism nehepatocytes kunobvumira vaongorori kufanotaura hafu - hupenyu uye zvinogona kuparara zvigadzirwa zvezvinhu izvi, izvo zvakakosha mukugadzirwa kwezvinodhaka. Chimiro chemetabolism chemukomboni chinogona kuona kwete kushanda kwayo chete asiwo kuchengetedzeka kwayo, sezvo metabolites dzimwe nguva inogona kuratidza huturu husingaonekwe mukomboni yemubereki.
Hepatotoxicity, kune rumwe rutivi, inobatanidza kukuvadza kwemakemikari zvinhu pamasero echiropa. Kushandiswa kwezvose zviri zviviri zvekutanga uye zvakagadzirirwa hepatocytes mune zvidzidzo zvehupfu zvinobatsira mukujekesa nzira dzinotungamirira kukukuvara kwechiropa. Izvi zvakakosha nekuti chiropa, chiri chepakati chemetabolism, chinotapukirwa zvakanyanya nekutuka kune muchetura kubva kune ese makemikari ezvemamiriro ekunze uye emishonga. Zvidzidzo zvinoshandisa masero aya zvinogona kuona maitiro ekukuvadzwa kwesero kana kusashanda zvakanaka, zvichipa ruzivo rwezviitiko zvekutanga zvekukuvara kwechiropa uye kubatsira kufanotaura migumisiro yakaipa mumamiriro ekliniki.
Enzyme induction chimwe chinhu chakakosha chakabatana nebasa rehepatocyte, zvichireva maitiro ayo zvimwe zvinhu zvinowedzera kuumbwa kwezvinodhaka-metabolizing enzymes. Ichi chiitiko chinonyanya kukosha sezvo chinogona kutungamirira kukuwedzera kwemetabolism ye-co-administered zvinodhaka, zvingaita kuderedza kushanda kwavo kana kuwedzera kugadzirwa kwemetabolites inokuvadza. Tsvagiridzo uchishandisa plateable hepatocytes inowanzo kutariswa kuongorora nzira dzekutonga dzinotonga enzyme induction, nekudaro ichijekesa kupindirana kwakaoma pakati peakasiyana xenobiotics uye chiropa simba remetabolism.
Hepatic siRNA Delivery uye Targeting Strategies
Kufambira mberi kwazvino muRNA interference (RNAi) therapeutics yakaunza nzira nyowani dzehepatic target deliverykushandisa maRNA madiki anopindira (siRNAs). Nzira yakakosha inosanganisira conjugating siRNA molecules neN - acetylgalactosamine (GalNAc), iyo inobatsira kusarudzwa kutorwa nehepatocytes kuburikidza neasialoglycoprotein receptor (ASGPR) ASGPR inoratidzwa zvakanyanya pamusoro pehepatocytes uye inoita basa repakati mu endocytic internalization ye.GalNAc-siRNAconjugates, zvichiita kuti ive nzira yakakosha yechiropa-yakananga kuendesa zvinodhaka.
In vitro zvidzidzo zvinowanzo shandaPrimary Hepatocyte siRNA Transfectionmatekiniki ekuongorora kugona uye kujeka kwesiRNA GalNAc Hepatocyte Delivery. Nzira idzi dzinobvumira vatsvakurudzi kuti vadzidze maitiro ekugogodza kwemajini mumamiriro ezvinhu ane chokuita nehupenyu uye kukwidziridza magadzirirwo ekutumira kuti akwanise kutora zvakanyanya intracellular uye kunyarara kwemajini.
Pakazere, Hepatic Targeted Delivery yeRNA therapeutics uchishandisa ASGPR-mediated GalNAc-siRNA masisitimu inomiririra yakanyatsojeka uye isiri - yemavhairasi nzira yekurapa majini echiropa zvirwere uye metabolic kusagadzikana, zvichiwedzera kusimbisa kushandiswa kwemaprimary hepatocyte modhi mune zvese tsvakiridzo yekushandura uye kugadzirwa kwezvinodhaka.
Zvisikwa-Zvakajeka Hepatocyte Details
Human Hepatocytes
Plateable Human Hepatocytes zvakakosha pakufanotaura nezvemetabolism yemishonga yevanhu uye chepfu, ichipa kushanda kwakanyanya. Kunamatira kwavo pamahwendefa akasarudzika kunobvumira kudzidza kwe metabolic enzyme induction, chikamu I / II metabolism, uye chiitiko chemutakuri.
Muchimiro chisina - chinoteedzera,Kumiswa hepatocytes yevanhu kurumidza kupindura kune xenobiotics, ichiita kuti ive yakakodzera kukurumidza kuongororwa kwemasabolism, zvidzidzo zvekubvisa, uye pfupi-yenguva yekuongorora toxicological.
Kwete-Munhu Primate Hepatocytes
Kufanana kwenyama kune vanhu kunoitaPlateable Cynomolgus Monkey HepatocytesuyePlateable Rhesus Monkey Hepatocytes yakakosha kubatanidza zvidzidzo zvepreclinical nemhedzisiro yevanhu. Iwo anoshandiswa kuongorora zvinodhaka metabolism, enzyme induction, uye marudzi - chaiwo ekuchengetedza profiles.
Kumiswa Cynomolgus Tsoko HepatocytesuyeKumiswa Rhesus Monkey Hepatocytes gonesa kukurumidza, kuenzanisa kuongorora kwemabasa emagetsi uye mhinduro dzakasimba, dzinobatsira kana pfupi - data renguva pfupi rinodikanwa pamberi penguva refu - zvidzidzo zvenguva paPlateable mafomati.
Canine Hepatocytes
Inoshandiswa zvakanyanya mukutsvagisa mhuka uye dudziro,Plateable Imbwa HepatocytesuyePlateable Beagle Hepatocytestevedzera mu vivo chiropa physiology yekudzidza zvinodhaka-induced chiropa kukuvara uye metabolism yezvipfuyo mishonga.
Izvi zvinopa zvidzidzo zvemetabolism nekukurumidza uye zvinoshandirwa mukutanga - nhanho yekuongorora assays, iyo inobatsira kubva nekukurumidza kutendeuka muiyo Suspension modhi.
Sprague-Dawley Rat Hepatocytes
IzviPlateable Sprague-Dawley Rat Hepatocytesakaparadzaniswa kubva kuSprague - Dawley makonzo uye anogadzirwa mune inoteedzera kana Plateable fomati. MuPlateable nzira, masero anonamatira kune tsika dish, iyo inovabvumira kuumba yakagadzikana intercellular kudyidzana uye kuchengetedza chiropa-chaiwo mabasa kwenguva yakareba. Iyi fomati inonyanya kukodzera kudzidza refu-yenguva metabolic maitiro, enzyme induction, uye chisingaperi toxicological ongororo uchishandisa Sprague-Dawley rat chiropa maseru.
Kana yakachengetedzwa mune isiri-inotevera mamiriro,Kumiswa Sprague-Dawley rat hepatocytesanoshandiswa kunyanya kwenguva pfupi-yedzo yenguva pfupi. Iyo Suspension fomati inofambisa kukurumidza kuyedza, senge acute zvinodhaka metabolism zvidzidzo uye enzyme kinetics ongororo, uko nekukurumidza kushanda kwemhinduro kunodiwa. Iyi modhi inobatsira kana uchienzanisa acute metabolic mhinduro pakati pemarudzi akasiyana kana mamiriro.
ICR/CD-1 Mouse Hepatocytes
Yakaparadzaniswa kubva kuICR/CD-1 mbeva, idzi Plateable ICR/CD-1 Mouse Hepatocytesvanoomerera kune tsika ndiro, zvichivabvumira kuchengetedza zvakakosha zvechiropa kwenguva yakareba. Vatsvagiri vanofarira iyo Plateable nzira yeICR/CD-1 mbeva hepatocytes kana yakagadzikana, yakawedzera tsika nguva ichidikanwa pakuongorora.
Kusiyana neizvi, Suspension ICR/CD-1 mbeva hepatocytes inogadzirwa pasina kuomerera, nokudaro ichichengeta yakatenderedzwa morphology yakakodzera pfupi-yenguva zvidzidzo.Kumiswa ICR/CD-1 Mouse Hepatocytesinobvumira kuongororwa nekukurumidza kwemhinduro dzemetabolism uye inonyanya kubatsira mukutarisisa kwekutanga kana kana yakakwirira-yedzo yekubuda ichidikanwa. Kushandiswa kweSuspension hepatocytes mu ICR / CD - 1 mice inobatsira kukurumidza kugadzirisa zvinyorwa zvemagetsi uye zvienzanise kune mamwe marudzi kana mamiriro akasiyana ekuedza.
Feline Hepatocytes
Iyo Plateable fomati inonyanya kubatsira kune refu - zvidzidzo zvenguva refu zvinoda chiropa chakasimba - chaiwo mabasa, akadai seasingaperi toxicity ongororo uye enzyme induction kuyedza. Muzvidzidzo zvakatarisana nezvirwere zvechiropa, zvinodhaka - kukuvara kwechiropa, uye kusagadzikana kwemetabolism yakanangana nekatsi,Plateable Cat Hepatocyteskupa muenzaniso wakasimba. Kuomerera kwavo kunosimudzirawo kuberekana kwe morphological uye inoshanda magumo, zvichiita kuti ive inosarudzika modhi mune veterinary pharmacology uye hepatology research.
Iyo Suspension fomati inogonesa kuyerwa kwemhinduro dzekukurumidza cellular kune xenobiotics, uko kushaikwa kwekunamatira kunoderedza kunonoka kwemhinduro kunoonekwa mune refu-temu tsika.Kumiswa Cat Hepatocytes saka zvakakosha pakuziva shanduko dzekutanga dzebiochemical uye zvingangove zvakashata zvinokonzeresa zvichitevera kuratidzwa kune matsva makomisheni.
Chicken Hepatocytes
Sevamiriri vemhando dzeavian,Plateable Chicken Hepatocytesanoshandiswa mukuenzanisa zvidzidzo kuti anzwisise kusiyana kwekushanduka-shanduka mumabasa echiropa, kunyanya mu endocrine disruptor assessments.
Kumiswa huku Hepatocytesinogonesa kukurumidza, acute - chikamu chezvidzidzo zvechiropa metabolism mushiri uye inogona kuenzaniswa zvakananga nevanoyamwisa.
Zvimwe Zvisikwa
Kune mamwe marudzi, Plateable Minipig Hepatocytes, Kumiswa Minipig Hepatocytes, Plateable New Zealand White Rabbit Hepatocytes, Kumiswa New Zealand White Rabbit Hepatocytes,Kumiswa Wistar Rat Hepatocytes,Kumiswa Wistar Han Rat Hepatocytes ese ariwo mamodheru akajairwa ekufanotaura nekutsanangura zvinodhaka-kusangana kwezvinodhaka uye kudzidza cytotoxicity yezvinodhaka..
Mhedziso
Hepatocytes inoita basa rakakosha mukuchengetedza kushanda kwechiropa kuburikidza nemetabolism, detoxification, kugadzirwa kwebile, uye protein synthesis. Chinyorwa chinosimbisa mutsauko pakati pekutanga hepatocytes uye mafomu avo akagadzirwa-plateable uye kumiswa hepatocytes-imwe neimwe yakagadziridzwa kune akasiyana ekuedza zvinangwa. Plateable hepatocytes, nekuomerera kwavo uye kurarama kwenguva refu, zvakakosha pakudzidza zvisingaperi zvinokonzerwa, enzyme induction, uye kwenguva refu - metabolic process, nepo kumiswa hepatocytes inopa kukurumidza uye yunifomu yekuyedza mamiriro ekuyedza acute. Mukuwedzera, kuongorora kunosvika kune marudzi-mashandisirwo chaiwo, achiratidza maitiro akasiyana-siyana ehepatocyte-kubva kuvanhu kusvika kune mhuka-rubatsiro mukufanotaura nezvemetabolism yezvinodhaka, kuongorora hepatotoxicity, nekugadzira mishonga yakananga yakadai seRNA-kupindira kwekushandisa uchishandisa GalNAc conjugation. Iyi mhedziso yakazara inoratidza basa rakakosha rehepatocytes mune zvese zvekiriniki tsvakurudzo uye pharmacological development, ichipa ruzivo rwakakosha muchiropa physiology uye nzira dzinoita kuti zvinodhaka kuchengetedzwa uye kushanda.
Nguva yekutumira: 2025-04-15 10:57:26