Maqaqailaneng a petso ea pedogolo: mefuta eohle le ho itšehla thajana, ho bata, liteishene tsa ho phela.

Mantsoe a bohlokoa:Mabenyane (spls); Cynomolgus monkey Splenostes; Rhes Splenocytes; Lintja masapo; Canplenocytes; Rat splenocytes; Mabenyane a mabe; Litoeba maqaqailana; Masene a mmutla; Splenocytes Ho bata maqaqailana; Ho supa masene

Sehlahisoa sa Iphase

Mabenyane (scls)ke batho ba bangata ba li hatetsoeng ke baphonyohi ba ikemetseng ho tloha spleria, e leng setho sa bohlokoa ho sefa mesebetsi ea ho itšireletsa mafung, le ho boloka lapeng. Lisele tsena li kenyelletsa li-lymphocytes (TSS, lisele tsa B), lisele tsa tlhaho), ke lisele tsa tlhaho, kaofela ha lisele tse tebileng. Liphoso li sebelisoa haholo mosebetsing oa ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta ho ithuta

Karolo le sebopeho sa masene (scls)

Ka har'a sengoane, masoli a hae a lula libakeng tse tloaelehileng: pulp e khubelu, e nchafatsang mali e khubelu ea mali, le makhasi a masoeu, moo likarabo tsa 'mele li qalisoang. Li-pulp tse tšoeu li hlophisitsoe hore li be libaka tsa cell le B tsa ho tsamaisa litšebelisano lipakeng tsa Antigen - Ho hlahisa lisele le li-lymphoctes. Meetso ena e ikhethang e etsa hore masapo (spls) sesebelisoa se matla se kang Elispot, se phallang butle, le li-lymphocyte li ikutloa joang.

Mefuta ea Mefuta ea Mefuta e Ikhethileng ea Mabotho (SPLES)

-Cynomolgus monkey Splenostes:Litšoene tsa Cynomolgus li sebelisoa lipatlisisong tsa khale hobane li ntse li letsahalla mesebetsi ea 'mele ea batho ba moea, e etsang monkey e belaetsang e loketseng lithuto tsa phetolelo.

-Rhes Splenocytes:Rhes Splenocytes ea Rhesus li bohlokoa ka tsela e tšoanang bakeng sa ho tšoana ha bona ba ho batla tsa 'Muso ka tsela e tšoanang. Boithuto bo sebelisang RHUS Splenostes Thuso ea Thuso ea Thuso ea Boroala e pakeng tsa mefuta ea liphoofolo le lits'ebetso tsa clinical.

-Ntja splenocytes / canplenocytes:Ha a ntse a etsa lipatlisiso tsa bongaka, lintja tsa masene

-Le masene a mmutla: Masene a mmutla a bohlokoa lithutong mabapi le tlhahiso ea antibody le nts'etsopele ea ente. Thuso ea Rplenocytes Thuso e senola lintlha tse ka fapaneng le li-splenocytes tsa temoso kapa li-splenocytes.

-Mouse Splenocytes / MIce Splenocytes:Boloi ba mese ke e 'ngoe ea masapo a masomi a neng a ithuta khafetsa (a spels) amtenoology. Li-Protocol tsa mashano a ho itšehla thajana li thehiloe hantle, 'me ka bobeli linyeoe le li-splenocytes le litlolo tsa litoeki li sebelisetsoa ho phalla cytometry, Elispot le assays le a mang a sebetsang.

-Rat Splenocytes:Rat Splenocytes Fana ka mohlala o tlatselletsang bakeng sa lithuto tsa lefu la immunotoxiflocology le livideo. Hangata, rapolese hangata ha ho bapisoa le maqeoa a teuse ho utloisisa liphapang tsa borena.

Bornocytes

BornocytesNa mohato oa ho tlosa lisele tsa 'mele ho tsoa ho bolokoe lisele tsa lipatlisiso, tse kang phallo ea lipalesa, ho fana ka tlhahiso ea cytokine.

Spleen e tlositsoe tlasa maemo a saptoptic, sebaka sa tharollo ea ho itšehla thajana mme o fetisetsoa thabeng ea Stermi 1640. Sekoko sa lekocyte se ile sa bokelloa ka hloko ka mor'a ho makala. Lisele li ile tsa hlatsuoa ka Rpmi 1640 Mediumum, Centrifuglud le ea hlonephang e lahliloe. Mohato ona oa ho hlatsoa o ile oa phetoa 1 - makhetlo a 2 pele lisele tse ikhethileng li sebelisetsoa liteko tse ling.

Li-splenocytes

Li-splenocyteske bohato ba bohlokoa bakeng sa ho boloka lisele bakeng sa tšebeliso ea nako e tlang. Ho lla ha bafuputsi ho boloka li-splenocytes bakeng sa nako e telele ntle le ho beha ts'ebetso ea bona kapa ts'ebetso ea bona.

Ho emisoa ha sele hantle hantle ho tloha mohatong oa ho itšehla thajana ho lahliloeng le ho tsepamisa mohopolo ho ile ha etsoa bo-ramandium. Kenya sejana se seng le se seng sa ho bata le ho fetisetsa setshelo se bataletseng, se netefatsa hore ha lisele li ntse li emisoa. Hang-hang ho theola lijana tsa ho bata kapele ho - 80 ° C Freezer. Fetisetsa ho - 150 ° C

Ho supa masene

Ho supa masenee tlameha ho etsoa ka hloko ho netefatsa sele e phahameng ea sele le ts'ebetso ea ho lla. Mokhoa oa ho tseba lintho o potlakile ho fokotsa litlamorao tse mpe tsa sebopeho sa DMSso le Sekhooa sa leqhoa.

Lits'ebetso li fetisetsoa ho bate ea metsi a 37 a C C, 'me e tlale feela ho fihlela likristale tsa lehloa feela li sala li le ka li-tubes. Eketsa 0.5 - 1ml ea setso sa Cele se bohareng ho lahloa le lehloa, hape le ho fetisetsa ho emisoa ho ba 15ml. Centrifege, tlosa Supernatant mme o tlanye mojaro ho lokolla tokisetso ea sele. Kenya 1ml of Cele Setso se mahareng, se koba le ho ipehela ka pipette, eketsa bohareng ho bongata ba 15 ml. Centriguge, de - Supernicatume, eketsa 1ml ea semelo sa sele, ho eketsa ho latela semela se lebelletsoeng. Beha lisele ho co2 incubator le ho kenella bakeng sa 1H e nang le lekhalo le lenyenyane ka sekwahelo. Qetellong ea incubation, e potlakisang 'me u tlohelle 1min ho lumella maloanfuba a nang le chelete e kenang ho opeloa. Ho emisoa ka seleng ntle le pula e fetiselitsoe ka hloko tube e ncha ea 15ml. Lisele li ne li baloa ho lekola ts'ebetso ea sele.

Sephetho

Maqeba a fapaneng, le karolo ea tsona e fapaneng le karolo ea bohlokoa mesebetsing ea tikoloho eo ke e bonang lipatlisiso tsa bohlokoa ea ho etsa joalo. Ebang ke litoeba, lipelong tse ling tse kang mebutlanyana le lintja, mashano a khokhothelang mosebetsing oa ho itšireletsa mafung. Mekhoa ea ho itšehla, ho bata, 'me ho lla haholo ho boloka botšepehi ba bona le boits'oaro, ba nolofalle bafumike ho hlahloba mefuta e fapaneng ka mefuta e fapaneng. Ha kutloisiso ea rona ea ho ikoetlisa e le kannete e lebisang ho bakoang ho koala lithuto le lits'ebeletso tsa bongaka tsa bongaka, ho netefatsa tsoelo-pele ea meriana ea batho le ea bongaka.