Tšimong ea lipatlisiso tsa biomedical le limolek'hule, ho hlokahala hore ho be le lintho tse ngata tsa sephara. Har'a libaka tsena,Tristosomes-Ea ka ho khethehaRat tritosomesFana ka leseli la bohlokoa liketsong tsa enzyatic, letso ea metabolic - liphetoho tse amanang le tsona, li ba etsa tse bohlokoa makaleng a batho ba etsang lithuto tse ling.

Sengoliloeng sena se hlahloba lintho tse bontšang lintho tse ratoang, 'me li arohantsoe,' me li tšoantšoa, le hore na ke hobane'ng ha li bohlokoa bakeng sa lipatlisiso tse ngata tsa mahlale.

Ho utloisisa li-tritosomes: organelle e ikhethang

Li-tritosomes ke subsosomes, e leng membrane - li-organels tse kentsoeng lits'ireletso tse mpe tsa macroLecules. Le ha maemo a tloaelehileng a na le li-enzyme tsa hydrolytic, li ruiselitsoe hydrolase tse khethehileng tsa tlhaho ebe li bontša litšobotsi tse tloaelehileng tsa biochemical tse li khethollang ho baahi ba tloaelehileng ba bo-zasompani ba ba tloaetseng.

Polelo ea "Triotiase" e ile ea qala ho tsebisoa lithutong tse latelang tse latelang tse sebelisang litsoi tse fapaneng le tse nang le chelete e ngata. Ka ho sebelisa mekhoa e joalo, bo-ramahlale ba ile ba khona ho khetholla litlamorao tsa tritosome - tse kang tritosomes - tse fapaneng ka sebopeho, letsoalo le enzyme le tšebetsong.

Libakeng, li-tritosomes li ithutile ho fumaneha ka lebaka la setho - matšoao a khethehileng a enzyme le tlhahiso ea lisele tse haufi.

Ho itšehla thajana le sebopeho sa patitosomes

Ho itšehla thajana hangata ho ama motho ea kenang ka matsoho kapa ea Percoll Grandengeation e latelang ea lisele. Li-this tse sebete li sebelisoa hangata, kaha li-hepatocytes li na le litekanyetso tse ngata tsa boitšoaro bo bongata ba ngaus-zadomal.

Mehato ea Bohlokoa ea ho Khutsa e Truang e kenyelletsa:

• Homonized har sebete kapa lisele tsa menoana tlasa maemo a batang ho boloka bokoma-morao.
• Tekolo e fapaneng ea ho tlosa Nuclei le Mitochondria.
• Tekanyo ea matloana ea ho khetholla tritloanomes ho tsoa ho li-tritosomes tse tloaelehileng le li-vesicles tse ling.
• Enzyme assays ho khetholla tritosome - Mesebetsi e ikhethileng, hangata e sebelisa matšoao a joaloka Β - Mannosidase, kapa aryllusese.

Hang ha li-tritosomes li hlahlojoa, li hlahlojoa ka Miroscopy, e leng boipelaetso, Enzyme, e leng sebopeho sa ho ithuta sebopeho sa bona, sebopeho le sebopeho se sebetsang.

Hobaneng u sebelisa mefuta ea rat bakeng sa ho ithuta tritosomes?

Likhoto ke lintho tse ratoang tsa mohlala tsa lithuto tsa motsamao bakeng sa lithuto tsa bona tsa 'mele le metabolic ka lebaka la ho tšoana ho li tšoana le batho, ho bonolo ho sebetsana le ho sebetsana le tsona, le ho theha li-protocol tsa laboratori. Sebese sa Rat, ka ho khetheha ke mohloli o motle oa enzsomal e kenang hammoho le ho ba mehlala e tšepahalang bakeng sa ho ithuta, lysosomal matla.

Li-tritosomes li na le thuso e bohlokoa haholo ho etsa lipatlisiso tse shebileng pele ho:

• Mafu a polokelo ea lysosomal
• Botsofaling le Senecence
• Lithaba - metabolism e tobileng
• Ho senyeha ha metabolism le chefo

Ho arohana ha li-tistosomes ho tsoa ho lisele tsa rat ho li entse mohlala oa motheo oa ho ithuta oa ho ithuta ka lebaka la mafu a bohlokoa a holofetseng.

Likopo tsa lithuto tsa patlito tse bohlale ho lipatlisiso tsa biomedical

1. Mathata a polokelo ea Lysosomal (LSDs)

Li-tritosomes li hlophiselitsoe li-enzyme tse khethehileng tse sa tsotelleng kapa li nyamela ka LSDS. Ho ithuta li-organele tsena ho thusa ho hlakisa hore na bothata ba enzyme bo lebisa ho subrationconction e bokelloang.

Mohlala, bafuputsi ba sebelisitse tritosomes ho utloisisa letšeng la Erzymaric ho sitisoa ke maano a enzzhetic, a fana ka leseli la maano a enwanente ea enzyme.

2. Ho tsofala le ho putlama ho phopholetsa

Age - liphetoho tse amanang le tšebetso ea Lysososomal ke letšoao la botsofali. Lithuto tse bohlale tsa ka libaka tse seng li sa majeke li senotse ho bokella liposhouscin, tšebetso e fetotsoeng ea enzyme le autoctian e bonolo ea lisentisi tsa Sendense.

Sena se etsa hore rat li-patitomes ke sesebelisoa sa bohlokoa bakeng sa lipatlisiso Age - Tsamaiso e amanang le Khale e amanang le e amanang le ba amanang le semelo sa neurodegeneration le mesifa ea mesifa.

3. Tokollo le phaello le pharmacotics

Lysosomes, ho kenyelletsa tritosomes, li kenya letsoho tiiso ea tatellano le khatello ea li-Xenobios. Ka ho ithuta li-tritosomes, litsebi tsa chefo li ka utloisisa joang hore na joang Lithethefatsi le chefo ea tikoloho li sebetsoa ka boemo ba mile.

Liphatlalatso tsa Ketsahalo ea Enzyme ho li-tritosomes li ka boela tsa thusa ho bolela bocha ke metabolism e tobileng ea lithethefatsi, e tšehetsa kholo ea metsoako e sireletsehileng ea phano e sireletsehileng.

4. Seleng ea lisele e bapisang

Rat Tritosomes e lumella bafuputsi ho bapisa boitšoaro ba rysosomal ho fapana le mefuta le lisele. Sena se na le thuso haholo ha ho ithuta mefuta - enzyme Kinzyme ea Kinzymen

Mekhoa ea Tlhahlobo e sebelisitsoeng ho lipatlisiso tsa Tritomar

Boithuto ba lipallo tsa rat bo sebelisa mekhoa ea metsamao ea khale le ea sejoale-joale:

• Enzyme ketsahalo ea ho nka tlhasetso ea bongata ea hydrolases.
• Ho kenella ka bophirima le maano a Bophirimela bakeng sa tlhaiso ea protheine.
• Boikutlo ba ho itšireletsa le microscopty ea Croncorcol ea lithuto tsa lehae.
• Lipiokics ho lekola sebopeho sa membrane le lipaterone tsa polokelo.

Lisebelisoa tsena li thusa bafuputsi ba ho ngola likarolo tse sebetsang tsa li-tritosomes ka lebaka la pelsiology ea selefouno le pathophysiology e nang le phapang e phahameng.

Meeli le Boikutlo ba Bokamoso

Ha re ntse re batla lipatlisiso tse fanoeng mabapi le leseli le leholo, mefokolo e mengata e sahloa:

• Heterogeneity of Lysosomes e ka thatafatsa toloko ea sephetho sa litlamorao.
• Mefuta ea mefuta ena - ho lokela ho nkuoa e le tlhaiso-leseling e eketsehileng ho feta ea biology ea motho.
• Ho leka-lekana ha li-protocol ho hlokahala ho ntlafatsa ho tsoaloa ha maqeba.

Ho sa tsotellehe mathata ana, thahasello ea li-tritosomes tse ntseng li hola, haholo-holo maemong a khatello ea maikutlo, metabolism le matšoao a ho itlhahloba. Patlisiso ea bokamoso e ka tsepamisa maikutlo ho biosensors Bokatokotso kapa live - mekhoa ea ho ithuta tsa lisele ho ithuta li-Tritomal ka nako ea sebele.

Sephetho

Rat Tritosomes e emela sebopeho se ikhethang le se rutang se phethang karolo ea bohlokoa ho biology ea khale. Ka ho khetholla le ho tšoaea li-tšetsetso tsena, bafuputsi bana ba khonne ho tebisa kutloisiso ea bona ea Melaobolia, Age - Ho Qhetsa ho Amabola le Mekhoa e amanang.

Ha sechaba sa mahlale se ntse se tsoela pele ho hlahloba tse ling tsa mokhatlo oa subclus, Rat Tritosomes e tla lula e le mohlala oa ho anyesa mekhoa ea ho ikatisa le litakatso tsa bona bakeng sa bophelo bo botle le maloetse bakeng sa bophelo bo botle le maloetse bakeng sa bophelo bo botle le maloetse bakeng sa bophelo bo botle le mafu.