Lijo-Serum ea Mouse e Bakiloeng ke Botenya le Plasma

Keyword: Diet-Induced Obesity mouse serum, Diet-Induced Obesity mouse plasma, DIO mouse serum, DIO mouse serum, DIO mouse plasma, lipatlisiso tsa botenya, lipatlisiso tsa lefu la metabolism, pharmacokinetics, tlhahlobo ea biomarker, nts'etsopele ea mokhoa oa ho hlahloba, ho netefatsa mokhoa oa ho hlahloba, bioanalysis

IPHASE Sehlahisoa

Selelekela

Lijo-botenya bo bakoang ke botenya (DIO)toeba ke mefuta ea liteko e sebelisoang haholo lipatlisisong tsa lefu la metabolism. Ka ho fepa toeba lijo tse phahameng - tse mafura kapa tse phahameng - lik'halori, bafuputsi ba ka theha phenotype e ts'oanang hantle le botenya ba motho, ho kenyelletsa boima ba 'mele bo eketsehileng, ho hanyetsa insulin, ho se mamelle tsoekere, dyslipidemia, le ho ruruha ho sa foleng ho tlase - Ka lebaka la litšobotsi tsena, hoMoetso oa mouse oa DIOhangata e sebelisoa lithutong tsa botenya, lefu la tsoekere la mofuta oa 2, lefu la sebete le se nang mafura a joala, mafu a pelo le methapo ea kutlo.

Lijo-Serum ea Mouse e Bakiloeng ke Botenya leDiet-Induced Obesity Mouse Plasma ke lipalo tsa bohlokoa tsa baeloji bakeng sa lipatlisiso tsa pele ho mefuta ena. Ba fana ka mohlala- lisampole tse tsamaellanang bakeng sa tlhahlobo ea biomarker, tlhahlobo ea bongata, le nts'etsopele ea liteko lithutong tse amanang le metabolism e fetotsoeng le likarabo tsa ts'oaetso ea systemic.

Lijo-Moetso oa Mouse o Bakiloeng ke Botenya

TheMoetso oa mouse oa DIOe bopilwe ka ho kena dipakeng tsa dijo tsa nako e telele ho ena le ho fetola diphatsa tsa lefutso, ho e etsa mohlala o sebetsang le o tenyetsehang bakeng sa dipatlisiso tsa metabolism. Ho fapana le mefuta ea botenya ba monogenic, litoeba tsa DIO li bonts'a hantle tšebelisano e rarahaneng lipakeng tsa lijo, tikoloho le taolo ea metabolism e bonoang bathong.

Hangata litoeba tsena li bonts'a ho bokellana ha mafura 'meleng, metabolism e sa tloaelehang ea lipid, le ho se sebetse hantle ha glucose homeostasis. Lithutong tse ngata, toeba ea DIO e boetse e amahanngoa le liphetoho ho li-adipokine, li-cytokine, li-enzyme tsa sebete, le metabolite e potolohang. Ka lebaka leo, mohlala o loketse haholo bakeng sa ho batlisisa tsoelo-pele ea botenya-mathata a amanang le metabolism le ho hlahloba batho ba khahlanong le botenya kapa ba khahlanong le lefu la tsoekere.

Hobaneng ha Lijo - Serum ea Botenya e Bakiloeng ke Botenya le PlasmaTaba

Serum le plasma ho tsoa ho litoeba tsa DIO ke lipalo tsa bohlokoa tsa tlhaho hobane li bonts'a boemo ba metabolism ea phoofolo. Hangata mehlala ena e sebelisoa ho lekanya tsoekere, insulin, leptin, adiponectin, triglycerides, mafura a mafura a mahala, k'holeseterole, matšoao a ho ruruha, le maloetse a mang-li-biomarker tse amanang le tsona.

Lipatlisisong tsa metabolic, mohlala - serum e ts'oanang le plasma e ka ntlafatsa bohlokoa ba lithuto tsa bioanalytical. Li bohlokoa haholo ha ho hlahlojoa metsoako e amang ho leka-lekana ha matla, taolo ea lipid, metabolism ea tsoekere, kapa matšoao a ho ruruha. Ntle le moo, ba ts'ehetsa ts'ebetso ea tlhahlobo e ka hlahisoang hape ho li-workflow tsa tlhahlobo le tse netefalitsoeng.

Lisebelisoa ea DIO Mouse Serum le Plasma

DIO Serum ea mouse le Plasma li sebelisoa haholo liphuputsong tsa pele le tsa phetolelo hobane li bonahatsa boemo ba tsamaiso ea metabolism ea mohlala oa botenya. Li-matric tsena li bohlokoa haholo lithutong tse etselitsoeng ho lekola ho se sebetse hantle ha metabolism, karabelo ea kalafo, le lefu - liphetoho tse amanang le biomarker.

Lithutong tsa pharmacokinetic le toxicokinetic, DIO mouse plasma e atisa ho sebelisoa ho lekanya litekanyetso tsa lithethefatsi tse potolohang le ho lekola boemo ba ho pepeseha, ho monya, le ho hlakola litlaleho tsa anti-bobesity, anti-diabetic, le metsoako e meng e amanang le eona. Lithuto tsena li thusa bafuputsi ho utloisisa hamolemo hore na lithethefatsi tsa mokhethoa li sebetsa joang maemong a fetotsoeng a metabolism.

Ho nts'etsopele ea mekhoa ea tlhahlobo le netefatso, DIO mouse serum le plasma li sebelisoa hangata joalo ka mohlala-matrices a bohlokoa bakeng sa LC-MS/MS, ELISA, le li-platform tse ling tsa bongata. Ba ka ts'ehetsa ntlafatso ea mekhoa, ho lokisoa ha curve ea calibration, tlhahlobo ea phello ea matrix, tlhahlobo ea ho hlaphoheloa, le tlhahlobo ea ho ikatisa.

Tlhahlobong ea biomarker le ea baeloji, matrices ana a sebelisetsoa ho lekanya matšoao a metabolism le a ho ruruha joalo ka tsoekere, insulin, leptin, adiponectin, triglycerides, cholesterol le cytokines. Litlhahlobo tse joalo li na le thuso bakeng sa ho ithuta tsoelo-pele ea botenya, ho hanyetsa insulin, ho senyeha ha lipid, le phekolo - liphetoho tse bakoang ke boemo ba metabolism.

DIO mouse serum le plasma le tsona li sebelisoa hangata lithutong tsa ts'ebetso, mochini-oa-lipatlisiso tsa liketso, le lipatlisiso tsa phetolelo bakeng sa botenya, lefu la tsoekere la mofuta oa 2, NAFLD, le mathata a mang a metabolism. Ka ho fana ka mehlala-e tsamaellanang le disampole tsa baeloji, li thusa ho ntlafatsa bohlokoa le ho toloka ha data ea preclinical.

Qetello

Diet-Induced Obesity Mouse Serum le Plasma ke matrices a bohlokoa bakeng sa lipatlisiso tsa metabolism, nts'etsopele ea lithethefatsi, le tlhahlobo ea biomarker. E le mohlala- lisampole tse sebetsang ho tsoa ho DIO mouse system, li ts'ehetsa lithuto tsa pharmacokinetic, nts'etsopele ea mokhoa oa tlhahlobo, netefatso ea mokhoa oa tlhahlobo, tlhahlobo ea bioloji, le tlhahlobo ea baeloji mabapi le botenya - lipatlisiso tse amanang.

Ka bohlokoa ba bona bo matla ho lefu la metabolism ea batho, matrices a litoeba tsa DIO ke lisebelisoa tsa bohlokoa bakeng sa bafuputsi ba ithutang botenya, lefu la tsoekere, dyslipidemia, ho ruruha le mathata a mang a metabolism.