Aikace-aikace na Ruwan Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙwararrun Ƙwararrun Ƙwararru (ACSF) ta yi a Fasahar Matsala ta Patch

Mahimman kalmomi:Ruwa na wucin gadi na cerebrospinal, ACSF, CSF wucin gadi, Ruwan cerebrospinal na roba, CSF Synthetic

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Gabatarwa

Ga masu bincike a cikin neurophysiology, Artificial Cerebrospinal Fluid (ACSF) yana ɗaya daga cikin mahimman abubuwan da ake amfani da su akai-akai a cikin gwaje-gwajen manne. Nisa daga kasancewa mafita na baya-bayan nan, CSF Artificial CSF ta bayyana ma'anar mahimmancin miliyon na waje wanda ke ƙayyadad da kwanciyar hankali, sake haifuwa, da kuma dacewa da ilimin lissafi na rikodin electrophysiological.

Manufar gwaje-gwajen mannen faci shine a haifuwa, kamar yadda zai yiwu, yanayin aikin in vivo na neurons a ƙarƙashin yanayin ex vivo. A cikin wannan gwaji, CSF Artificial yana aiki azaman babban tsarin tallafi. Abun sa na ionic, iyawar buffering, osmolarity, da ma'aunin iskar gas kai tsaye yana tasiri yuwuwar yuwuwar hutun neuronal, yuwuwar harbe-harbe, watsa synaptic, da halayen gating na tashar.

Tushen Jiki na Ruwan Cerebrospinal na Asalin

Duk ƙa'idodin ƙira da aikace-aikacen CSF Artificial an gina su akan ingantaccen fahimtar ayyukan ilimin lissafi na ruwan cerebrospinal na asali.

Ruwan cerebrospinal na asali shine bayyanannen ruwa na physiological wanda ke kewaye da tsarin juyayi na tsakiya, yana samar da ingantaccen microenvironment mai mahimmanci don ayyukan neuronal na yau da kullun. Koyaya, saboda sauye-sauye a cikin abun da ke ciki da iyakantaccen dama, ba za a iya dogaro da CSF na asali a cikin saitunan gwaji masu sarrafawa ba. Don magance wannan, An haɓaka ruwa na cerebrospinal na roba don yin kwafin mahimman kaddarorin physicochemical na CSF, yana ba masu bincike damar kiyaye daidaito, yanayin da ya dace da ilimin lissafi a cikin in vitro da ex vivo nazarin.

Farashin ACSF a matsayin Mahimmin Reagent a cikin Gwaje-gwajen Matsala

A cikin gwaje-gwajen manne, Ruwan Artificial Cerebrospinal Fluid shine ainihin maganin extracellular. Neurons da glial Kwayoyin a cikin tsarin juyayi na tsakiya suna ci gaba da nutsewa a cikin CSF na asali a cikin vivo, da kuma yuwuwar su na huta, aikin yuwuwar samar da aikin, tashar tashar ion, da sakin vesicle synaptic sun dogara sosai akan matakan ion, pH, osmolarity, da wadatar kuzari.

Makasudin yin rikodin mannen facin shine a sake haifar da in vivo yanayin ilimin halittar jiki da aminci kamar yadda zai yiwu a ƙarƙashin yanayin ex vivo. A matsayin simintin wucin gadi na CSF na asali, CSF Artificial CSF yana ba da ingantaccen microenvironment don keɓancewar nama da ƙwayoyin jijiyoyi, kuma a yawancin ayyukan aikin lantarki ba za a iya maye gurbinsa da matsakaicin al'adu ko saline mai sauƙi ba.

Aikace-aikace a cikin Fasahar Matsala ta Patch

Ana amfani da CSF na wucin gadi cikin cikakken aikin gwaje-gwajen manne, daga shirye-shiryen nama zuwa rikodi da sarrafa magunguna.

1. 1) Gwaje-gwajen Maɗaukakin Ƙwaƙwalwar Ƙwaƙwalwar Yanki

Yayin rarrabawar kwakwalwa da slicing, ya kamata a canza nama da sauri cikin kankara - sanyi, oxygenated ACSF (95% O₂ / 5% CO₂). Ƙananan zafin jiki yana taimakawa jinkirin metabolism na neuronal, yayin da daidaitaccen iskar gas yana kula da kwanciyar hankali na pH kuma yana rage lalacewar hypoxic.

Bayan slicing, sassan kwakwalwa yawanci ana sanya su a cikin ACSF mai iskar oxygen a 32-34 ° C na 1-1.5 hours don ba da damar dawowa aiki. A wannan mataki, abun da ke ciki na ionic, osmolarity, da samar da makamashi (misali, glucose) na CSF Artificial CSF suna da mahimmanci don kiyaye amincin nama da kuma iyawar tantanin halitta.

Yayin rikodin electrophysiological, ya kamata a ci gaba da tura ruwa na wucin gadi na cerebrospinal don kula da tsayayyen yanayi na waje. Wannan yana tabbatar da daidaiton pH, ma'auni na ion, da zafin jiki, yayin da kuma cire sharar rayuwa, a ƙarshe yana goyan bayan ingantaccen abin dogaro da siyan bayanai.

2. 2) Gwaje-gwajen Maɗaukakin Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararru

Don rarrabuwar kawuna mai tsauri, naman kwakwalwa yawanci ana narkewa da ƙwayoyin cuta. Maganin enzymatic yawanci ana shirya shi bisa tushen ruwa na cerebrospinal na Artificial domin ana kiyaye tsarin membrane da aikin tashar ion yayin narkewa. Bayan narkewa da trituration na inji, keɓancewar neurons ana wanke su akai-akai tare da ACSF don cire ragowar enzyme sannan a sake dakatar da su a ACSF don hutawa ko mannewa. Stable osmolarity da pH suna da mahimmanci a cikin wannan tsari don hana raunin cell da apoptosis.

3. 3) Kwayoyin Al'ada da Layukan Kwayoyin Halitta

Don daidaitaccen daidaitaccen rikodin tantanin halitta da rikodi ɗaya - tashoshi, ana iya amfani da ruwa na wucin gadi na wucin gadi na yau da kullun azaman mafita na waje don adana yuwuwar membrane mai hutawa da yanayin yanayin tashoshi na ion.

Don keɓance takamaiman igiyoyin ionic, tsarin CSF Artificial galibi ana canza shi. Misali, lokacin rikodin igiyoyin calcium, ana iya maye gurbin wani ɓangare na NaCl da TEA-Cl don toshe tashoshi na potassium, ko ana iya cire sodium don murkushe igiyoyin sodium. Lokacin yin rikodin mai karɓar NMDA - madaidaicin igiyoyin ruwa, ana iya barin Mg2+ na waje don sauƙaƙe toshe tashar. A kowane hali, ainihin kayan aikin physicochemical na maganin dole ne su kasance da ƙarfi.

4. 4) Haɗe-haɗe na Ayyukan Aiki

Ana amfani da ruwa na cerebrospinal na wucin gadi don shirya mafita na aiki don masu canza tashar tashar ion da mahaɗan neuroactive a cikin nazarin ilimin harhada magunguna, waɗanda ake amfani da su ta hanyar tsarin lalata. Bambance-bambance a cikin pH ko abun da ke ciki na ionic na iya canza solubility na miyagun ƙwayoyi, kwanciyar hankali, ko ma daidaituwar sunadaran da aka yi niyya, wanda ke haifar da sakamako mara inganci.

A cikin gwaje-gwajen rikodi na haɗe-haɗe na optogenetic, aikin neuronal ana fara sarrafa shi ta hanyar ƙarfafa haske sannan kuma ana kula da shi ta hanyar mannewa. Ana buƙatar ci gaba da bugun jini tare da ACSF don kula da yanayin yanayin electrophysiological na asali, ta yadda canje-canjen da aka lura za a iya danganta su ga sa baki na optogenetic maimakon canjin yanayi.

La'akarin Fasaha da Mahimman Ma'aunin Gwaji

A cikin gwaje-gwajen manne, masu biyowa na Artificial Cerebrospinal Fluid - sigogi masu alaƙa yakamata a sarrafa su sosai don tabbatar da daidaiton sigina da sake haifuwa.

Gaba ɗaya, waɗannan abubuwan suna ƙayyade kwanciyar hankali na rikodi, sake fasalin bayanan, da kuma dacewa da ilimin halitta na sakamakon.

Kammalawa
Ruwan wucin gadi na cerebrospinal yana da yawa fiye da bayani na baya. A cikin fasahar clamp ɗin faci, tana aiki azaman tushen muhallin waje wanda ke tallafawa yuwuwar nama, yana adana kaddarorin electrophysiological, kuma yana ba da damar ingantaccen bincike na aiki. Haɓakawa a hankali na abun da ke ciki na ACSF da yanayin gwaji don haka shine tsakiya ga kowane babban aiki mai inganci na electrophysiology.

Don tallafawa waɗannan buƙatun, IPHASE yana samar da samfuran ACSF masu inganci waɗanda aka ƙera ƙarƙashin ƙaƙƙarfan ƙa'idodi masu inganci, tare da ƙayyadaddun tsarin ionic, osmolarity, da pH. Wannan yana tabbatar da yawa - zuwa - daidaito da yawa da ingantaccen aiki, yana taimaka wa masu bincike samun tabbataccen sakamako na gwaji.