Tib neeg ib qho ntshav siab ntshav mononuclean (pbmc)
PBMC tuaj yeem ua kom pom tseeb hauv Instrumental ib puag ncig los muab cov qauv hauv Instrical Instrumental rau kev tsim kev tshuaj. Kawm cov kev cuam tshuam ntawm cov tshuaj ntawm lub hom phiaj thiab phiajcim txoj hauv kev tuaj yeem pab nrhiav cov tswv yim tshiab thiab cov lus qhia uas tsis tau pom thiab kev txhim kho.
Cov lus qhia tseem ceeb ntawm PBMC hauv kev txhim kho tshuaj tiv thaiv tshuaj muaj xws li:
1.Thaum tus kheej - NKOJ COB, macrophages, thiab lwm yam yog cov effect Eyster PBMCs hauv Adcc Activ. Cov hlwb no tuaj yeem tua cov phiaj hlwb encapsulated los ntawm cov tshuaj tiv thaiv los ntawm kev khi rau lawv.
2.Mixed Lymphocyte Regents (MLR): Txheej Txheem System rau Thawj Cov Hlwb thiab T Cell - Kev Kho Mob T Cellource. DC cov cells nthuav qhia ntau ntau ntawm Pd - L1 nyob rau hauv lawv cov npoo, uas tuaj yeem khi thiab inhibit pd - 1 nyob rau saum npoo ntawm t hlwb. Yog tias cov tshuaj tuaj yeem thaiv cov kev tiv thaiv ntawm ob, tom qab ntawd nws tuaj yeem txhawb cov kev loj hlob ntawm T cov hlwb thiab yog li ua haujlwm ntawm cov tshuaj.
3. Cov kev ua haujlwm ntawm tes ntawm tes: T cell kev ua haujlwm yog ib qho ntawm cov khoom siv feem ntau rau kev ntsuas cov tshuaj ntawm cov tshuaj tiv thaiv kab mob ntawm cov tshuaj tiv thaiv kab mob. Nyob rau hauv t cell kev ua haujlwm tau, nyob rau hauv - Verro kev coj noj coj ua thiab tsim nyog tsim, xws li antigencies lossis tshuaj tiv thaiv, yog muab rau ntxias kev ua kom lub tshuab t hlwb.
4.T cov tshuaj uas muaj kev cuam tshuam ntawm cov tshuaj los ntawm kev sib piv cov tshuaj sib txawv ntawm cov neeg sib txawv nrog cov pab pawg kev sim nrog pawg tswj hwm.
Tib neeg pbmcs tsim los ntawm cov tshuaj ntsuab los ntawm tib neeg cov ntshav uas muaj feem los ntawm kev ua kom qis dua ntawm Centrifugation. Cov no yog ib qho nucleus hlwb tsuas yog tsim ntawm lymphocytes (t hlwb, thiab xaws hlwb) thiab monocytes.
▞ Cov Ntaub Ntawv Khoom:
|
Lub npe |
Yam khoom No. |
Kev qhia tshwj xeeb |
Ntawm tes ntawm tes |
Cia / Xa Khoom |
|
Tib neeg tib neeg cov ntshav liab qab mononucleal |
082A01.11 |
5 lab nyiaj / ml |
Tshiab |
2 - 8˚C cia, cov khoom xa tuaj |
Pro Daim Ntawv Thov Khoom Muag:
Nyob rau hauv - vitro metabolism kawm txog cov tshuaj.
