Taw qhia rauLier microsomes
Cov microsomes me me vesicular tawg tau los ntawm endoplasmic reticulum thiab pom nyob rau hauv daim siab cell (Hepatocytes). Lawv ua lub luag haujlwm tseem ceeb hauv ntau cov txheej txheem biochemical, feem ntau yog nyob rau hauv cov tshuaj metabolism thiab detoxification. To taub cov qauv, muaj nuj nqi, thiab qhov tseem ceeb ntawm daim ntawv meter microsomes yog qhov tseem ceeb rau kev ua tsaug, kev lom zem rau kev mob ntsws.
Cov qauv thiab tsim ntawm microsomes
● Keeb kwm los ntawm endoplasmic reticulum
Microsomes tau tsim thaum lub sijhawm homogenization thiab enterrifugation ntawm cover hlwb, qhov twg endoplasmic reticulum so rau hauv cov hlwv me me. Tus txheej txheem no tso cai rau kev rho tawm ntawm cov txheej txheem sib cais, uas muaj cov nplua nuj nyob hauv cov enzymes thiab tseem ceeb rau ntau yam kev ua si metabolic.
● Sib xyaw thiab morphology
Lier micromomes muaj feem ntau ntawm daim nyias nyias - khi enzymes, lipids, thiab cov protein. Lawv cov qauv tsim lawv los pab txhawb ntau cov tshuaj tiv thaiv ntau, suav nrog cov catalyszed los ntawm Cytochrome P450 enzyme system. Cov yam ntxwv morphological ntawm microsomes yog qhov tseem ceeb hauv kev txiav txim siab lawv lub peev xwm ua haujlwm hauv cov kev ntsuas metabolic.
Micreemomes thiab tshuaj metabolism
Hole Lub luag haujlwm hauv Detoxification
Lier micromomes yog qhov tseem ceeb rau lub cev muaj peev xwm ua kom detoxify ntau lub tebchaw, suav nrog tshuaj thiab ib puag ncig cov co toxins. Cov txheej txheem detoxification cuam tshuam nrog kev hloov pauv lipophilic mus rau hauv dej ntau dua - soluble cov ntaub ntawv uas tuaj yeem tawm tau yooj yim ntawm lub cev.
● Cytochrome P450 enzyme system
Ib qho ntawm cov tseem ceeb tshaj plaws hauv daim siab micromomes yog Cytochrome P450 (CYP) enzyme system. Cov enzymes no yog lub luag haujlwm rau cov metabolism ntau ntawm cov substrates, suav nrog cov tshuaj, suav nrog ua ib lub luag haujlwm tseem ceeb hauv tshuaj metabolism. Cov kev ua ntawm cov enzymes no tuaj yeem cuam tshuam txoj kev ua tau zoo thiab tshuaj lom ntawm cov tshuaj.
Micromomal enzymes thiab lawv cov haujlwm
● Hom enzymes tam sim no
Lier microtomes muaj ntau hom kev sib xyaw, suav nrog oxidases, rov ua dua, thiab hydrolases. Txhua hom enzyme tau tshwj xeeb hauv kev saib xyuas cov kev coj noj coj ua biochemical tshwj xeeb, ua rau lub zog metabolic ntawm lub siab.
● Cov kev tawm tsam tshwj xeeb catalyzed
Cov enzymes nyob rau hauv daim siab micreem saib cov tshuaj sib txawv, xws li oxidation, txo qis, hydrolysis, thiab kev sib txuas. Cov kev tawm tsam no tseem ceeb rau cov biotransformation ntawm endogenous teb thiab exogenous tebchaw, kom lawv pov tseg kom zoo metabolic.
Lub luag haujlwm hauv xenobiotic metabism
● Kev rhuav tshem cov tshuaj txawv teb chaws
Cov microTal nyob hauv cov tshuaj xenobolizing xenobolizing-txawv teb chaws uas nkag rau hauv lub cev, xws li tshuaj, carcinogens, thiab cov pa phem. Los ntawm cov txheej txheem ntawm enzymatic, microsomes hloov cov khoom muaj teeb meem uas muaj kev phom sij thiab ntau dua cov ntaub ntawv yooj yim.
● Qhov tseem ceeb hauv chaw muag tshuaj thiab toxicology
Lub peev xwm ntawm daim siab micromes rau metabolize xenobiotics muaj qhov cuam tshuam tseem ceeb hauv chaw muag tshuaj thiab tshuaj lom. Nkag siab rau microsomal kev ua haujlwm pab qhia cov tshuaj sib cuam tshuam tshuaj, thiab cov kev sib txawv hauv cov tshuaj metabolism, thiab cov tswv yim tshuaj thiab kho cov tswv yim tshuaj.
Microsomes hauv Lipid metabolism
● Synthesis ntawm lipids
Liver microsomes ua lub luag haujlwm tseem ceeb hauv Lipid metabolism, suav nrog synthesis ntawm phospholipids thiab triglycegides. Cov lipids no yog cov khoom tseem ceeb ntawm cov tshuaj txhuam ntawm tes thiab ua haujlwm rau lub zog ntawm cov microsives hauv kev tswj hwm kev ncaj ncees ntawm tes thiab lub zog sib npaug.
Role txoj haujlwm hauv cov roj (cholesterol) thiab steroid metabolism
Microsomes tau koom nrog hauv Biosynthesis thiab kev tswj hwm ntawm cov roj (cholesterol) thiab sterid cov tshuaj steroid. Cov enzymes nyob rau hauv microsomes pab txhawb kev hloov pauv ntawm cov roj cholesterol rau ntau cov txheej txheem sterterol, uas yog qhov tseem ceeb rau kev zom zaub mov thiab kev sib kho mob thiab kev sib deev.
Kev cai ntawm microsomal ua
● Cov yam ua tau zoo rau kev ua haujlwm enzymatic
Ntau ntau yam muaj peev xwm cuam tshuam cov kev ua ntawm microsomal enzymes, suav nrog kev sib txawv caj ces, hnub nyoog, poj niam txiv neej, thiab lub xeev kab mob. Ib qho ntxiv, sab nraud yam xws li kev noj zaub mov noj, ib puag ncig raug, thiab muaj lwm yam tshuaj muaj peev xwm hloov cov haujlwm enzyme, cuam tshuam cov kev ua haujlwm ntawm lub siab ua haujlwm thiab cov tshuaj metabolism.
● Kev cuam tshuam ntawm kev noj haus thiab ib puag ncig
Kev noj zaub mov thiab ib puag ncig ua si lub luag haujlwm tseem ceeb hauv kev tswj hwm microsomal. Piv txwv li, qee yam zaub mov tuaj yeem ua rau muaj kev ua si ntawm cytochrome p450 enzymes, ua rau variations hauv cov metabolism thiab ua hauj lwm zoo. Cov co rau ib puag ncig kuj tseem tuaj yeem cuam tshuam rau microsomal ua haujlwm, cuam tshuam rau daim siab muaj peev xwm ua rau detoxify cov tshuaj tiv thaiv.
Pathological hloov hauv microsomes
Kev cuam tshuam ntawm cov kab mob siab
Mob siab cov kab mob siab xws li mob siab, mob siab, thiab cov kab mob siab tuaj yeem ua rau hloov kho hauv microsomal enzyme kev ua thiab qauv. Cov kev hloov no tuaj yeem cuam tshuam lub siab muaj peev xwm metabolize tshuaj thiab lwm yam tshuaj, ua rau muaj kev cuam tshuam nrog kab mob siab thiab kev teeb meem ntawm kev teeb meem.
● Hloov pauv vim haus dej caw lossis quav yeeb tshuaj
Kev siv ntev thiab quav yeeb tshuaj tuaj yeem tsim kev siv yeeb tawm ntawm daim siab micromomes, suav nrog enzyme induction lossis inhibition. Cov kev hloov pauv no tuaj yeem cuam tshuam rau lub siab lub peev xwm mus rau metabolize cov tshuaj, muaj peev xwm ua rau lom neeg thiab lub siab puas tsuaj.
Microsomes hauv kev tshawb fawb thiab kuaj mob
● Siv rau hauv kev kawm cov kev sib cuam tshuam
Lier micromomes tau ntau dua hauv cov kev tshawb fawb ua ntej los kawm cov tshuaj sib cuam tshuam kev siv tshuaj thiab kwv yees li cov kev sib cuam tshuam metabolism. Cov kws tshawb nrhiav siv cov teeb meem micromes los soj ntsuam lub peev xwm rau kev siv yeeb tshuaj yeeb yaj kiab, thiab txheeb xyuas cov ntaub ntawv metabolic, muab cov ntaub ntawv metabolic, muab kev ntsuas kev nyab xeeb rau kev tsim tshuaj thiab kev nyab xeeb.
● Cov ntawv thov hauv chaw kuaj mob
Hauv kev kuaj mob kuaj mob, daim tawv tawv microsomes ua cov cuab yeej tseem ceeb rau kev ntsuas cov nplooj siab muaj nuj nqi thiab kuaj xyuas cov metabolic cuam tshuam. Kev kuaj mob Micreem tau muaj peev xwm txheeb xyuas enzyme tsis muaj peev xwm lossis kev txawv txav, kev kho mob kho kom zoo.
Yav Tom Ntej Cov Lus Qhia thiab Kev Tsim Kho
● Kev tawm tswv yim hauv microsomal
Kev tshawb fawb txuas ntxiv hauv thaj teb ntawm microsomal biology txuas ntxiv mus qhia txog kev pom zoo tshiab rau hauv kev ua haujlwm thiab kev tswj hwm ntawm daim ntawv ntsuas micromomes. Kev nce qib hauv Genomics, cov proteomics, thiab metabolomics yog txhim kho peb txoj kev nkag siab ntawm microsomal enzymes thiab tus kab mob rau kev kho mob tshiab thiab tshuaj kho mob tus kheej.
● Kev tawm thev naus laus zis thiab cov txheej txheem
Kev tsim kho thev naus laus zis thiab cov txheej txheem teeb meem tau tsim los txhim kho txoj kev kawm ntawm daim ntawv ntsuas micromes. Siab - Kev Ntsuam Xyuas Kev Ntsuam Xyuas, Kev Ua Si Cov Txheej Txheem Bientechnological yog kev tshuaj xyuas cov khoom siv microsomoticly ntau dua thiab muab cov qauv kev tshawb pom ntawm cov tshuaj tshiab thiab cov tswv yim.
Hais txog Iphase
Lub tsev nyob hauv North Wales, Pennsylvania, Athe Biosciences yog ib qho tshwj xeeb, tshiab, thiab kev tsim kho siab tshiab ua haujlwm, kev tsim khoom, kev lag luam ntawm cov tshuaj tiv thaiv roj ntsha tshiab. Kev paub ntau yam thiab kev nyiam ua rau kev tshawb fawb, peb pab kws tshawb fawb thoob plaws hauv lawv cov hom phiaj kev kawm thoob plaws hauv lawv cov hom phiaj kev tshawb fawb. Kev nrhiav tus R & D zoo tshaj plaws ntawm "kev tawm tsam tshiab, tshawb nrhiav cov chaw nyob hauv Tebchaws Asmeskas, thiab cov neeg faib khoom lag luam muaj ntau dua 12,000 square metres.
Lub sij hawm Post: 2024 - 08 - 09 2014:12:10