Selelekela ho Rat Liver S9
Rat sebete S9Na sub - Setšoantšo sa ho sella se fumanoeng se tsoang ho lisele tsa sebete, haholo-holo ho tloha likhoto, ka ho latela mokhoa o ikhethileng oa homone le ho bokelloa. E na le motsoako oa li-enzyme le coftoctors tse tsoang likaroloana tsa lisele tsa sebete tsa microsolic. Rat sebete S9 o ts'oara bohlokoa bo boholo le ho etsa lipatlisiso tsa liphoofolo tsa boriti le tsa promatical bakeng sa karolo ea eona ea ho etsisa metabolism ea vivo. Bafuputsi ba sebelisa sebete sa ra rat
Likarolo tsa biochemical tsa rat sebete s9 |
Boemo ba enzymatic |
Rat sebete S9 o ruile ka enzymes tse fapaneng tse nkang karolo mohato ka bobeli. Tlhahiso-leseling e felletseng ea enzymatic e lumella mofuta oa Rate ea Rat ka ho ba mohlala oa ho ithuta ho ithuta ho ithuta metabolism ea lithethefatsi. |
Metabolic Pataways e amehang |
Litsela tsa metabolic tse bokelloang ke raling tsa ran Ketso e kopaneng ea litsela tsena e thusa ho tlosa Xen hooobios le ho fetola prodrugs liforomo tsa tsona tse sebetsang. | |
Ho itokisetsa le ho tlosoa ha Rat Liver S9 |
Mekhoa ea laboratori |
Hangata barekisi ba sebelisa li-buffers ba kenyelletsa tris - Plassium phossphate, hangata e tlatsetsoa ka coftors joalo ka Nadph ho ts'ehetsa tšebetso ea enzyme. Ho boloka lisele tsa sebete ka mocheso o tlase ho pholletsa le ts'ebetso e bohlokoa ho boloka tšebetso ea enzzymatic. |
Likopo |
Lithuto tsa TOXCOPology |
Metabolism ea lithethefatsi:Ho Vitro Assays e sebelisa botsitso ra rat |
| Teko ea Carcinogenicity:Hangata karoloana ea S9 e kenyelletsoa litekong tsa bo-'mampoli joaloka tlhahlobo ea AMS, e ntseng e bona likotlo tsa DNA tse bakoang ke ho pepesetsoa ka lik'hemik'hale. | ||
Pharmacokinetics |
Karolo ea I le tso mabapi le bohato:Pharmacokitics haholo capyy e fana ka bokhoni ba lisebelisoa tsa Ratri S9. Ntho e ruileng ea enzzzzzyanatic e lumella bafuputsi hore e ithute ka bobeli ba eona le tsome maemo a mantlha a tsokolohong e laoloang. | |
| Lipolelo tsa moralo oa lithethefatsi:Ka ho supa ho tsoa ho metabolic hotspot le li-actimedia tse ka 'mele tse chefo pele ho ho ntlafatsa likarolo tsa lik'hemik'hale ho ntlafatsa sebopeho sa' mele sa metabolic le ho fokotsa chefo. | ||
Ho leka Makhooa a Tikoloho |
Metabolism ea Xenbolis:Rati sebete S9 ke sesebelisoa sa bohlokoa sa ho ithuta metabolism ea lik'hemik'hale tsa tikoloho, tse tsejoang e le li-Xenboobios. Litsela tsena, tse kenyelletsang chefo e bolaeang likokonyana, lik'hemik'hale tsa botsoalle le liporollo, li ka ba teng ka har'a biotran. Li-enzyme tse teng ka har'a li-labol S9 li ka etsisa lits'ebetso tsa metabolic tseo li-Xoneoticotic tsena li tla beng li etsoa VIOO, li fana ka leseli maemong a bona a nang le chefo e le 'ngoe. | |
| Teko ea tikoloho ea tikoloho:Ka ho sekaseka hore na lik'hemik'hale tsa tikoloho li ka bolela litholoana tsa bona tse bohloko, li-bitlactulation le litlamorao tsa liphoofolo tse hlaha le batho. |
Liphephetso le ntlafatso
Meeli le Liphephetso |
Ho fapana ha enzyme mesebetsing ea enzyme |
E 'ngoe ea liphephetso tse kholo tsa mathata a mantlha a sebelisa sebete sa rante s9 ke phapang ea enzyme pakeng tsa litokisetso tse fapaneng. Ho fapana hona ho ka hlaha liphapang tsa protocols ea ho teneha, bophelo bo botle le ba liphoofolo, le khatello e tobileng ea likhoto tse sebelisitsoeng. |
Litšitiso tsa Bohlale le Thuso |
Litšitiso tse 'maloa tsa mahlale li ka ama tšebeliso e atlehang ea rase ka mokhoa o sebetsang oa ho etsa lipatlisiso. Ho boloka tšebetso ea enzzymatic nakong ea polokelo ea enzzymatic nakong ea polokelo le ho sebetsana le ho tšoaea liphoso, joalo ka ho tšoaea liphoso tse nepahetseng tsa liteko tsa li-vitro. Bafuputsi ba hloka ho tloaela litaba tsa mathata a amanang le botsitso ba enzyme, maemo a data le litlhaloso tsa data. Ho hlola mathata ana ho hloka ho kopanya tsebo | |
Limakasine tsa morao tjena le litlolo |
Mekhoa e mecha ea mekhoa |
Tsoelo-pele ea morao-rao tokiso le ts'ebeliso ea rat li-Rat li lahliloe haholo ts'ebeliso ea eona e le lipatlisiso. Ho nchafatsa ho kenyelletsa nts'etsopele ea li-protocols tse sebetsang haholoanyane tsa ho qala ho fihlela mekhoa e phahameng ea ho hlahlobisisa ho khetholla ketsahalo ea enzyme hantle. |
Ntlafatso ea theknoloji ho Sno |
Lintlafatso tsa theknoloji, tse kang phahameng - Bopaki bo rarollang Mekhoa ena e lumella semelo se qaqileng sa metabolishene ea metabolic le tsebiso ea li-metabolic tsa metabolic. |
Nako ea poso: 2024 - 08 - 19 4:47:58