Molao-motheo
TSA IPHASEPBMC ea ho itšehla thajanas li ipapisitse le mokhoa oa khale oa density gradient centrifugation, o ipapisitseng le phapang ea ho lokisa lebelo la lisele tse fapaneng tsa mali ka har'a seaplane sa gradient ho fihlela karohano ea lisele. E kenyelelitsoe ka har'a kit ke tharollo e khethehileng ea karohano ea "density gradient" e etsang hore ho be le gradient e tsitsitseng nakong ea centrifugation. Ha sampuli ea mali ea anticoagulated e kenngoa butle-butle holim'a tharollo e arohaneng, lisele tse teteaneng tse kang lisele tse khubelu tsa mali le granulocyte li lula ka tlaase ho centrifuge tube, ha li-PBMC tse fokolang tse fokolang li emisoa pakeng tsa tharollo e arohaneng le plasma, ho etsa karolo e hlakileng, e tšoeu, e koahetsoeng ke maru ea lisele. Ka ho bokella ka hloko lera lena la lisele le ho hlatsoa ka mor'a moo le centrifugation, PBMC e phahameng e ka fumanoa.Kenyelletso e khutšoanyane
IPHASE's PBMC Isolation Kit ke sesebelisoa se etselitsoeng bafuputsi ho arola PBMC hantle maling a anticoagulated. Sekhahla se na le likarolo tse latelang:1.Karohano e sebetsang hantle ka ho fetisisa: tšebeliso ea karohano e optimized density gradient e tiisa karohano e potlakileng le e nepahetseng ea PBMC nakong ea centrifugation, ha e ntse e fokotsa tšilafalo ho tsoa mefuteng e meng ea lisele.
2.Bohloeki bo phahameng: ka mokhoa o motle oa ho arohana le mehato e latelang ea ho hlatsoa, ho ka fumanoa bohloeki bo phahameng ba PBMC, ho fana ka motheo o ka tšeptjoang oa setso sa lisele tse latelang, liteko tsa baeloji ea limolek'hule joalo-joalo.
3.Sterility le endotoxin e tlase: tharollo ea ho itšehla thajana le likarolo tse ling ka har'a kit li kentsoe kalafo e thata ea aseptic le maemo a tlase haholo a endotoxin, ho netefatsa hore ha ho na litšila tse sa batleheng tse tla hlahisoa nakong ea ts'ebetso ea ho itšehla thajana.
4.E sebetsa haholo: ha e lokele feela PBMC ea batho ho itšehla thajana, empa hape e fana ka lisebelisoa tse khethehileng tsa ho itšehla thajana tsa PBMC bakeng sa mefuta e meng ea liphoofolo (e kang tšoene, ntja, rat, mouse, joalo-joalo) ho finyella litlhoko tse fapaneng tsa lipatlisiso.
5.Ho bonolo ho sebetsa: Kit e na le litaelo tse qaqileng tsa ts'ebetso le lisebelisoa tse hlokahalang tse thusang, tse etsang hore mokhoa oohle oa ho itšehla thajana o be bonolo le o bonolo, esita le bakeng sa ba qalang ho qala kapele.
Mehlala ea tšebeliso
Lisebelisoa tsa IPHASE tsa PBMC tsa ho itšehla thajana li sebelisitsoe haholo lipatlisisong tse fapaneng tsa biomedical. Ka mohlala, lipatlisisong tsa immunology, bafuputsi ba ka sebelisa PBMC e arohaneng bakeng sa thuto ea ho hlophisa le ho sebetsa ea lisele tsa T, lisele tsa B le lisele tse ling tsa 'mele; lipatlisisong tsa oncology, PBMC e ka sebelisoa ho lekola boemo ba 'mele oa ho itšireletsa mafung ba bakuli ba hlahala le ho shebella lisele tsa 'mele tsa ho itšireletsa mafung. Ho feta moo, kit e sebelisoa haholo ntlafatsong ea ente, tlhahlobo ea lithethefatsi le masimo a mang.Qetello
Setsi sa ho itšehla thajana sa IPHASE sa PBMC se nkile boemo ba bohlokoa lefapheng la lipatlisiso tsa biomedical ka ts'ebetso ea eona e ntle haholo le boleng ba ts'ebeliso e pharalletseng. Ka tsoelopele e tsoelang pele ea mahlale le thekenoloji le tlhokahalo e ntseng e eketseha ea lipatlisiso, IPHASE e tla tsoela pele ho ikitlaetsa ho nts'etsopele ea lihlahisoa tse sebetsang hantle, tse hloekileng le tse bonolo-ho-sebelisa lihlahisoa tsa bioloji ho fana ka tšehetso e matla ho feta bakeng sa bafuputsi.Nako ea poso: 2024-07-27 16:30:36