thymus tissue Baetsi, Bafani, Feme
Re tsepamisitse maikutlo holim'a moelelo, eseng ka mokhoa o tloaelehileng. Re nka bohlokoa ba maikutlo a basebelisi le litlamorao tse sebetsang ho khothaletsa boqapi le boqapi.Re lumela hore lihlahisoa tse tšoanelehang ha se tsona feela lintlha. Boiphihlelo ba moreki ke sepheo sa mantlha.Re ikemiselitse ho ntlafatsa boleng le litšebeletso ho fihlela litlhoko tsa bareki ka thymus-tissue,CD3/CD28 T cell expansion kit, thapo mali mononuclear cell kit, C57BL/6 mouse serum serum, lisele tsa stromal. Re ikemiseditse ho lelekisa bokgabane ba ho aha lebitso, ho ikamahanya le metheo ya mantlha ya "tlhahiso ya indasteri, bokgabane bo boholo ho isa phethehong". Re ts'ehetsa tsamaiso e ntle, ts'ebetso e bonolo, 'me re hapa ts'ebelisano 'moho ka maikutlo a tšepahalang. Khampani e lula e latela boetapele ba lihlahisoa le boqapi. Re sebetsa ka mafolofolo boikarabello ba mokhatlo oa sechaba. Re ikitlaelletsa ho theha boleng ba nako e telele. Re ntse re phehella tsamaiso ea sejoale-joale ho netefatsa nako ea ho fana ka bareki. Ho feta moo, re lula re ntlafatsa ts'ebetso ea k'hamphani. Ho fa bareki lihlahisoa tsa boleng ke boikarabello ba rona. Boleng ke bophelo. Re tsoela pele ho ba e 'ngoe ea indasteri ea ho hatisa le ho daea ea boemo ba pele lefatšeng le tsoelo-pele e tiileng. Re khomarela tsoelo-pele e tloaelehileng le bareki. Tšebeletso ea rona ea boleng e mamella lihlahisoa tse nang le botumo bo phahameng. Lihlahisoa tsa rona - tsa boleng bo holimo li fa bareki ts'ebeletso e tšepahalang, ea profeshenale le e sebetsang hantle bakeng sarat spleen mononuclear cell, Hepatic Cells Kit, nako e telele - setso sa hepatocyte, Streptavidin Nanobeads kit.Lihlahisoa tse Amanang
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