Txoj Cai ntawm Comet Assay
Lub Comet Assay, tseem hu ua ib leeg - cell gel electrophoresis, yog cov txheej txheem los txiav txim genotoxicity los ntawm kev txheeb xyuas DNA strand puas. Nws tuaj yeem tshawb pom thiab ntsuas ntau npaum li cas ntawm DNA ib leeg thiab ob chav nick kev puas tsuaj hauv hlwb. Thaum muaj ntau yam endogenous thiab exogenous DNA puas yam ua rau cov cellular DNA strand so, nws superhelical qauv puas, thiab nyob rau hauv qhov kev txiav txim ntawm cell lysate, cell membrane, nuclear membrane thiab lwm yam membrane cov qauv raug puas, thiab intracellular proteins, RNA, thiab lwm yam diffuse mus rau hauv lub cell lysate, whereas lub nuclear DNA tsuas yog khaws cia rau hauv qhov chaw loj heev.Nyob rau hauv nruab nrab tej yam kev mob, DNA fragments tuaj yeem nkag mus rau hauv gel thiab migrate, thaum nyob rau hauv qhov kev txiav txim ntawm alkaline electrolytes, DNA yog de-helicalized thiab puas DNA tawg strands thiab fragments raug tso tawm. Vim hais tias ntawm cov me me txheeb ze molecular loj ntawm cov DNAs thiab cov alkaline denaturation rau hauv ib tug strands, qhov tsis zoo nqi DNAs tawm lub nuclear DNA tsiv mus rau lub zoo ncej thaum lub sij hawm electrophoresis, uas ua rau ib tug comet - zoo li duab, thaum lub undamaged DNA feem tseem spherical. Yog li ntawd, nws tuaj yeem raug cais ntxiv rau hauv nruab nrab comet test (PH = 8.4) thiab alkaline comet test (PH> 13) raws li qhov sib txawv pH qhov tseem ceeb ntawm electrophoresis tsis. Cov qub no tsuas yog siv los kuaj xyuas DNA ob npaug - txoj hlua tawg puas tsuaj, thaum lub sijhawm tom kawg muaj qhov rhiab heev dua thiab tuaj yeem siv los txheeb xyuas qhov me me ntawm ib leeg - strand thiab ob npaug - strand breakage puas.
Feem ntau, qhov kev puas tsuaj DNA loj dua, ntau txoj hlua thiab cov khoom tawg ua rau nws tsim, qhov me me ntawm nws qhov ntev, ntau DNA nws migrate nyob rau hauv tib lub electrophoretic tej yam kev mob, thiab ntev nws migration nrug. Yog li ntawd, qhov degree ntawm DNA puas nyob rau hauv ib tug neeg lub hlwb yuav txiav txim tau los ntawm kev ntsuas qhov optical ceev los yog ntev ntawm cov migrated ib feem ntawm DNA, yog li txiav txim qhov kev sib raug zoo ntawm lub acting koob tshuaj ntawm cov kev kawm thiab cov DNA puas.
IPHASE cov khoom
Yooj yim: Cov reagents thiab consumables yuav tsum tau rau ib leeg - cell gel electrophoresis yog muab thiab yuav siv tau ncaj qha, zoo heev shortening lub sij hawm sim.Qhov tseeb: Txhua yam ntawm cov khoom siv tau raug kuaj xyuas nruj, thiab cov txiaj ntsig kev sim yog qhov tseeb, txhim khu kev qha thiab muaj peev xwm rov ua dua tshiab.
Stability: cov khoom siv ruaj khov thiab yooj yim thauj thiab khaws cia.
Daim ntawv thov dav: Nws muaj ntau yam kev siv thiab tuaj yeem siv rau kev kuaj tshuaj toxicology ntawm zaub mov, tshuaj, tshuaj tua kab, tshuaj tua kab mob, khoom noj khoom haus, tshuaj pleev ib ce, khoom siv kho mob thiab lwm yam.
IPHASE tau koom nrog hauv kev tshawb fawb txog kev tshawb fawb reagents rau ntau xyoo, thiab tau tsim cov kab ntoo khaub lig- teb thiab ntau haiv neeg siab - kawg kev tshawb fawb cov cuab yeej nrog kev tshaj lij tshaj lij thiab lub siab tshiab. IPHASE muab cov cuab yeej tshuaj ntsuam xyuas kom raug thiab ua tau zoo txhawm rau txhawm rau yug cov tshuaj tshiab; nws tseem yog lub hnub qub ci ntsa iab nyob rau hauv qhov chaw tshawb fawb loj ntawm lub neej science, tas li muab cov kws tshawb fawb nrog cov ntaub ntawv tshiab, cov tswv yim tshiab thiab cov qab ntug tshiab, ua rau txhua qhov dhia hauv pem hauv ntej ntawm kev tshawb fawb tshawb fawb. Nyob rau hauv lub sophisticated arena ntawm genotoxicity kev tshawb fawb, IPHASE tau pom txawv txawv kev pom thiab ua kom tiav, launching ib tug series ntawm yooj yim thiab npaum cov kev daws teeb meem los ua kom txoj kev tshawb fawb complex smoother thiab ntau npaum.
Hnub tim: 2024-07-26 15:29:08