Iphas cov kev daws teeb meem rau Sirna Tshuaj Hauv cov metabolism tshawb fawb
Nucleic acid tshuaj, vim lawv cov txuj ci thev naus laus zis, tau ua lub ntsiab lus ntawm kev txhim kho tshuaj tshiab nyob rau xyoo tas los no. Nucleic Acid Tshuaj suav nrog RNA (SARRAs), MANIANTS (ASO) Ntawm cov no, sirna yeeb tshuaj, raws li lub hotspot nyob rau hauv nucleic acid kev tshawb fawb thiab kev loj hlob hauv lawv cov gene siab, thiab kev siv tau yooj yim. Lawv yuav tsum yog cov tshuaj muaj kev cia siab tshaj plaws rau kev txhim kho tshuaj tshiab tom qab cov tshuaj tiv thaiv me me.
- 1. Cov kws kho tshuab kev pom hauv Sirna Tshuaj Ua Haujlwm
RNA cuam tshuam me me (sirna Thaum synthesis, sirna nkag mus rau hauv tes ntawm Operocytosis. Ib feem ntawm Sirna Escapes lysosomal kev ua tsis ncaj thiab nkag mus rau hauv cytoplasm, uas nws tau sib xyaw ua ke rau RNACH - Indus ua kom cov tawv ncauj (lub cev nqaij). Tsis pub dhau Ris, tus Sirna unwinds rau ob qho strands: lub siab strand thiab antisense strand. Lub siab strand yog sai sai degraded nyob rau hauv cytoplasm, thaum lub sijhawm pob, khi rau cov kev ntxub ntxaug, qhib siab. Cov complex ces xaiv los khi rau lub hom phiaj mMNA, ua kom yooj yim thiab cov kev cuam tshuam tom ntej. Raws li qhov tshwm sim ntawm MRA defradation, qib kev qhia ntawm lub hom phiaj yog txo qis, ua rau gene reetrencing thiab inhibition ntawm kev txhais lus protein.
- 2. Sirna Foltabolism Kev Tshawb Fawb Txog Kev Tshawb Fawb
Hauv Vivo, cov yeeb tshuaj sirna yog cov nucleally metabolized los ntawm nucleales nyob rau hauv cov ntshav thiab cov ntaub so ntswg, tsis yog theem i thiab II metabolic enzymes nyob rau hauv daim siab. Tom qab cov txheej txheem hloov kho, tam sim no cov tshuaj sirna cov tshuaj pom kev txo cov metabolism hauv cov hlab ntshav. Feem ntau, feem ntau ntawm cov tshuaj sirna ua kom nrawm nrawm nrawm nrawm nrawm mus rau lwm cov ntaub so ntswg, qhov chaw uas lawv nyob hauv lub siab lossis lwm cov ntaub so ntswg. Rau hauv Vivo Metabolism kev tshawb fawb ntawm sirna tshuaj, feem ntau yog txheeb xyuas cov ntshav, cov zis, thiab lub hom phiaj nqaij (raum) los ntawm cov qauv tsiaj.
Txawm li cas los xij, thaum lub sijhawm thaum ntxov ntawm kev txhim kho thaum ntxov, muaj cov nqi sim kev sim ua ke, thiab cov nqi siab uas cuam tshuam nrog loj - nplai kev tshuaj ntsuam thiab kev ua kom zoo. Raws li qhov tshwm sim, hauv Vitro metabolism kev tshawb fawb yog qhov tseem ceeb tshwj xeeb thaum pib theem ntawm Sirna Tshuaj Txhim Kho. Cov kev tshawb fawb no muaj cov txiaj ntsig zoo, xws li siab dhau thiab sim ua kom nrawm nrawm, uas tuaj yeem txhim kho Sirna Tshuaj Ntsuam Xyuas.
Cov Lus 1: Hauv Vitro Me Kev Tshawb Fawb Txog Kev Tshawb Fawb rau Sirna Tshuaj
| Substrate | Daim ntawv thov |
| Ntshiab / Ntshav |
Ntsuas cov metabolic stabability ntawm sirna pours hauv cov hlab ntshav thiab thoob plaws hauv lub system. Qhov no feem ntau yog qhov yuav tsum tau kuaj rau hauv vitro SiRO SUAV cov tshuaj yeeb. |
| Mob siab s9 | Muaj feem ntau ntawm cov enzymes pom nyob hauv daim ntawv teev cov ntaub so ntswg thiab yooj yim nkag. Rau qee yam, nws tuaj yeem siv los hloov chaw rau daim siab daim tawv nqaij homogenates. |
| Daim siab daim tawv nqaij homogenate | Lub kaw lus enzyme muaj ntau dua thiab raug pom zoo rau hauv vitro tshuaj ntsuam thiab ntsuas ntawm cov tshuaj lom. |
| Hepatocytes | Lub kaw lus enzyme yog qhov ua tiav ntau dua, ua rau nws haum rau kev tshuaj xyuas metabolic ntawm daim siab - vwj siab sirna yeeb tshuaj. |
| Lysosomes | Lysosomes yog thawj qhov chaw uas yog cov tshuaj uas sib ntsib Sirna ntsib tom qab nkag mus hauv cov hlwb ntawm Enderocytosis. Lawv muaj cov nplua nuj enzyme, suav nrog Nucleases thiab ntau lub vev xaib rau Sirna Metabolism. Lysosomes muab cov txheej txheem sim ua tau zoo rau kev kawm metabolic stability ntawm sirna yeeb tshuaj. |
- 3. IPlase cov khoom lag luam cuam tshuam
Cov koom haum koom nrog hauv cov khoom lag luam tsim khoom lawv cov ntaub ntawv los ntawm kev cai lij choj, nrog cov keeb kwm ntshiab.
Kev xyuas xim
Ob txoj haujlwm ntau lawm thiab tsiaj cov tsiaj twv txiaj ntsig los ntsuas cov khoom kom zoo thiab muaj kev nyab xeeb.
Siab huv
Cell Purity tuaj yeem ncav cuag 90%.
Siab muaj peev xwm
Kev siv xov tooj ntawm tes muaj peev xwm ncav cuag ntau dua 85%, ntsib cov neeg siv khoom xav tau.
Kev Rov Qab Qib Siab
Cov yaj thim rov qab tus nqi tuaj yeem tshaj li 90%.
Tib neeg
Muaj cov kev pabcuam kev cai muaj nyob ntawm cov neeg siv khoom, muab cov tsiaj tshwj xeeb lossis cov ntaub so ntswg cell customization.
| Pawg | Cov khoom IIhase |
| Cheebtsam | Mob siab Lysosomes |
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| Thawj Hepatocytes | Raug tshem tawm Hepatocytes |
| Platable hepatocytes | |
| Ntshav | Ntshav ruaj khov |
| Ntshav cov protein khi |
Lub Sijhawm Post: 2024 - 12 - 20 13:08:46