Dab tsi yog ua rau s9?

Taw qhia rauKev Ua Si S9

Kev tsim txom S9 yog daim siab - muab tau los ntawm enzyme fraction feem ntau siv nyob rau hauv toxicology thiab biochemical kev tshawb fawb. Nws tau npaj los ntawm kev sim siab mob ntsws hauv cov tsiaj, xws li cov nas lossis hamssters, nrog tshuaj pleev li tshuaj lom neeg zoo li Aroclor 1254, phenobarbital, lossis β - naphhoflavone. Qhov feem no yog qhov tseem ceeb hauv kev tshawb fawb venro, tshwj xeeb hauv kev ua haujlwm metabolic, raws li nws mimics cov metabolism ntawm xenobiotics nyob rau tib neeg lub cev.

Raws li ib tug thawj coj ntawm kev siv hluav taws xob tsim hluav taws xob tsim nyog,Iphase BiosciencesTshwj xeeb hauv kev tshawb fawb, kev txhim kho, kev tsim khoom, thiab faib tawm ntawm cov khoom siv hluav taws xob siab, suav nrog ua rau S9. Lub taub hau wales, Pennsylvania, Ipase Biosciences tau tsim ntau lub chaw R & DEO, thiab faib cov chaw muag khoom thoob plaws Suav teb, Tebchaws Asmeskas, Tebchaws Asmeskas, thiab cov tebchaws Asia sab hnub tuaj. Peb lub hom phiaj yog rau cov kws tshawb fawb thoob ntiaj teb hauv lawv cov kev ntsuas kev tshawb fawb, muab cov khoom siv hluav taws xob ua kom nrawm nrawm hauv kev kuaj yeeb tshuaj thiab kev kawm lom neeg.

Yuav ua li cas yog tsim txom s9 npaj?

Kev tsim txom S9 yog tsim los ntawm cov txheej txheem zoo -

1. Enzyme induction: Tsiaj (feem ntau yog nas) tau kho nrog enzyme - inducing sib txuas xws li Aroclor 1254 los txhim kho Cytochrome P450 enzyme kev ua si.
2. Daim siab rho tawm: Tom qab tawm tsam, cov hlab ntaws cov ntaub so ntswg tau sau thiab homogenized.
3. Centrifugation thiab Kev Ua Yeeb Yam: Homogenized lub siab yog centrifuged kom cais cov s9 feem, uas muaj qhov tseem ceeb metabolic enzymes.
4. Tswjhwm Zoo Thiab Cia: Cov khoom kawg nyob rau hauv kev sim nruj kom ntseeg tau tias qhov kev ua tiav zoo tshaj plaws ua ntej tau ntim thiab khaws cia thaum muaj kev ruaj khov.

Cov ntawv thov ntawm Indused S9

Kev ua rau S9 muaj ntau cov ntawv thov hla teb kev sib txawv ntawm cov chaw tshawb fawb:

• Kev Tshawb Xyuas Yeeb Tshuaj Yeeb Tshuaj: Siv los tshawb xyuas qhov kev ua lag luam li cas hauv lub cev.
• Kev sim tawm Mutagenicity: Plays lub luag haujlwm tseem ceeb hauv lubAmes xeem, qhov twg nws yuav pab txheeb xyuas kev xaiv mutagenic sib txuas los ntawm kev ua haujlwm metabolic.
• Carcinogenicity thiab Kev Tshawb Fawb Kev Tshawb Fawb: Pab rau hauv kev tshuaj ntsuam xyuas rau cov neeg muaj peev xwm carcinogens thiab cov tshuaj lom hauv cov tshuaj lom neeg.
• Kev ntsuas kev nyab xeeb thiab tshuaj lom neeg: Pab ntsuas kev nyab xeeb ntawm kev lag luam tshuaj thiab ib puag ncig cov pa paug.

Iphase Biosciences muab siab - purity, siab - kev ua haujlwm S9 cov zauv feem, Enabling cov kws tshawb nrhiav kom ua tiav cov txiaj ntsig raug thiab ntseeg tau rau cov kev tshawb fawb no.

Mechanism ntawm kev ua ntawm kev ua txhaum cai S9

Kev tsim txom S9 feem ntau ua haujlwm los ntawm:

• Theem kuv metabolism: Cytochrome P450 enzyme system oxidizes, txo qis, lossis hydrolyzes xenobiotics, ua rau lawv muaj ntau dua.
• Teve II Metabolism: Cov tshuaj tiv thaiv kab ntsig (piv txwv li (piv txwv li, glucuronidation, sulfation) txhim kho cov dej solubility kom yooj yim dua. Los ntawm kev ua yeeb yam ntawm cov txheej txheem metabolic, ntxias s9 yog qhov tseem ceeb rau kev kawm cov tshuaj tiv thaiv tshuaj thiab muaj peev xwm ua kom muaj tshuaj lom.

Cov hom kev ntxias S9 feem ntau

Muaj ntau hom kev ua txhaum cai s9 cov zauv feem uas ua rau cov kev xav tau tshwj xeeb:

• Kev tsis pub tus nas lub siab s9: Cov feem ntau siv feem hauv genotoxicity thiab Ames kev xeem.
• Kev Tawm Tsam Hamster Liiver S9: Muab ib txoj kev ua haujlwm metabolic ua kom muaj zog rau kev sib piv.
• Aroclor - Txim S9: Tshwj xeeb tsim nrog Aroclor 1254 los txhim kho cov kev ua si metabolic enzyme, siv dav hauv kev ntsuas toxicity.

Qhov zoo thiab cov kev txwv ntawm kev siv ua rau S9

Qhov Zoo:

• Muab cov txheej txheem txhim khu kev qha rau kev kawm metabolism hauv vitro.
• Txhim kho qhov tseeb nyob rau hauv kev kwv yees tshuaj yeeb tshuaj.
• Pab tswj kev ua raws li kev cai tswj hwm yuav tsum muaj rau kev ntsuas kev nyab xeeb.

Txwv:

• Tsis tag nrho cov kev sib tw ntawm cov metabolism hauv tib neeg.
• Kev ua enzyme yuav sib txawv raws li kev npaj npaj.
• Kev ruaj khov thiab kev cia khoom yuav tsum tau nruj me ntsis tswj tau.

Yuav Xaiv Qhov Ncauj Lus S9 rau koj qhov kev tshawb fawb

Thaum xaiv S9, Xav Txog:

• Qhua qhov chaw: RAT lossis Hamster Li lub siab cov lias muab cov kev sib txawv enzyme cov profiles.
• Inducing Cov Neeg Sawv Cev: Aroclor 1254 thiab lwm yam tseem ceeb cuam tshuam kev cuam tshuam rau enzyme.
• Xav Tau Kev Xav Tau: Ames xeem, yeeb tshuaj metabolism, lossis tshuaj lom tshuaj lom zem yuav tsum muaj cov enzyme tshwj xeeb.

Ntawm Iphase Biosciences, peb muab cov kev taw qhia thiab customized daws teeb meem xaiv cov neeg tshawb xyuas zoo tshaj plaws rau lawv cov kev sim. Peb cov khoom yauv ua nruj siv cov kev tswj hwm zoo kom ntseeg tau cov kev ua kom siab enzyme thiab sib txawv.

Tag

Kev tsim txom S9 yog cov cuab yeej tseem ceeb hauv Toxicology, chaw muag tshuaj, thiab kev nyab xeeb kev nyab xeeb kev nyab xeeb. Los ntawm kev ua kom zoo nkauj metabolic, nws pab cov kws tshawb fawb paub txog mutagenic, carcinogenic, thiab cov tshuaj lom. Raws li ib tus neeg ua haujlwm pabcuam ntawm cov neeg ua haujlwm lom neeg roj ntsha, Iphase biosciences tau cog lus los muab siab rau cov neeg ua hauj lwm s9 rau lub chaw ua hauj lwm thoob ntiaj teb.

Xav paub ntau ntxiv txog peb cov khoom s9 cov khoom lag luam lossis tso ib qho kev txiav txim, mus saib peb lub vev xaib lossis hu rau peb pab pawg niaj hnub no.