Cov txiaj ntsig tsis txaus siab? Qhov tshiab version ntawm cov khoom siv kuaj hauv vitro micronucleus nyob ntawm no los pab!

PART 01. In-Vitro Micronucleus Test

Micronuclei yog tag nrho cov chromatids los yog acentric fragments los yog lub nplhaib chromosomes uas tseem nyob rau hauv lub cytoplasm thaum chromosomes tsis tu ncua nkag mus rau cov ntxhais hlwb los tsim nuclei tom qab mitosis. Vim hais tias cov fragments los yog chromosomes tsis tuaj yeem nkag mus rau hauv lub ntsiab nucleus ntawm telophase, thaum cov ntxhais hlwb nkag mus rau lwm qhov interphase, lawv condense mus rau hauv me me nuclei sab nraum lub ntsiab nucleus thiab tsim micronuclei. Nrog rau elucidation ntawm kev tsim mechanism, qhov tseem ceeb ntawm micronuclei, kev txhim kho txuas ntxiv ntawm txoj kev tshawb pom thiab kev ua tiav ntawm cov txheej txheem sim, micronucleus test tau dhau los ua ib txoj hauv kev tseem ceeb rau kev tshuaj ntsuam kev hloov pauv. Nws yog dav siv rau kev kwv yees qhov kev pheej hmoo ntawm carcinogenic, kuaj xyuas aneuploidy inducers thiab lwm yam kev phom sij ntawm caj ces.

Kev kuaj hauv vitro micronucleus yog ib txoj hauv kev kuaj genotoxicity rau kev kuaj xyuas qhov tsim ntawm micronuclei hauv cov qe ntshav tom qab kho nrog cov tshuaj ntsuam xyuas. Nws yog qhov zoo tshaj plaws rau kev tshuaj xyuas chromosome breakage thiab aneuploidy induced hauv mitotic cells thaum / tom qab raug rau cov tshuaj ntsuam xyuas. Piv nrog rau hauv - vivo xeem, qhov kev kuaj hauv vitro micronucleus yog qhov yooj yim dua, sai dua, thiab pheej yig dua. Tsis tas li ntawd, nws tsis cuam tshuam los ntawm cov xwm txheej xws li tus kheej sib txawv ntawm cov tsiaj sim. Txij li thaum nyob rau hauv - vitro micronucleus kuaj tuaj yeem kuaj xyuas cov tshuaj tiv thaiv micronucleus tshwm sim los ntawm qee cov tshuaj ntawm cov ntsiab lus qis nrog kev nkag siab zoo. Yog li ntawd, nws tau dav siv nyob rau hauv lub teb ntawm genetic toxicology.


PART 02. In vitro micronucleus test kit

IPHASE in-vitro micronucleus test kit siv Suav Hamster Lung (CHL) hlwb ua qhov kev xeem. Cov hlwb CHL raug rau cov tshuaj ntsuam xyuas nrog / tsis muaj cov kab mob metabolic thiab kho nrog actin polymerization inhibitor cytochalasin B. Cov hlwb raug tso cai kom ncav cuag lawv qhov zoo tshaj plaws zaus ntawm binucleated hlwb, thiab tom qab ntawd sau, npaj, thiab stained; Tom qab ntawd lub micronucleus tus nqi ntawm cov hlwb uas ua tiav ib qho mitosis (binucleated hlwb) raug tshuaj xyuas nyob rau hauv lub tshuab ntsuas ntsuas los ntsuas qhov muaj peev xwm mutagenic ntawm cov tshuaj ntsuam xyuas. Cov khoom siv IPHASE muab tag nrho cov tshuaj tseem ceeb thiab cov hlwb rau kev kuaj hauv vitro micronucleus. Txij li thaum tag nrho cov khoom siv tau dhau qhov kev sim ua tau zoo, cov kev sim ua tau zoo yog qhov tseeb, txhim khu kev qha thiab rov tsim dua.

--Product Advantages --

1. Yooj yim

Txuag lub sij hawm rau induced S9 thiab reagent npaj,. Cov khoom siv tuaj yeem siv ncaj qha, ua rau lub voj voog sim luv luv.

2. Qhov tseeb

Txhua feem ntawm cov khoom siv reagent tau raug soj ntsuam nruj, yog li cov txiaj ntsig kev sim siv cov khoom siv yog qhov tseeb, txhim khu kev qha thiab muaj peev xwm ua tau zoo.

3. Kev ruaj ntseg

Cov khoom siv ruaj khov thiab yooj yim thauj thiab khaws cia.

4. Ntau yam

Kev siv ntau yam, tuaj yeem siv rau kev tshuaj ntsuam genetic toxicology ntawm cov khoom noj, tshuaj, tshuaj tua kab, tshuaj tua kab mob, khoom noj khoom haus, tshuaj pleev ib ce, thiab lwm yam.

Kev sib piv ntawm cov khoom siv tshiab thiab qub

IPHASE ua raws li lub teb chaws cov qauv thiab cov lus qhia rau kev kuaj hauv vitro micronucleus hauv ntau qhov chaw raws li nws cov kev taw qhia. Ntxiv mus, IPHASE yuav siv cov lus teb rau cov neeg siv khoom raws li nws lub hom phiaj kev ua kom zoo los kho qhov muaj pes tsawg leeg thiab cov txheej txheem ntawm cov khoom thiab tau ua tiav cov khoom siv hauv vitro micronucleus kuaj kom tau raws li cov neeg siv khoom xav tau ntau dua txawm tias tom qab kev tshuaj xyuas tas li.

1. Cov khoom sib xyaw

Giemsa staining reagents thiab reagents xav tau rau cov txheej txheem kev npaj tau suav nrog ua kom yooj yim dua thiab ua haujlwm tau zoo.

2. Txoj kev

Cov txheej txheem ntawm cov khoom siv yog cov ncauj lus kom ntxaws, nrog rau cov kev ntsuas ceev faj thiab lwm cov ntsiab lus kom tsis txhob muaj qhov yuam kev.

PART 03. Yam khoom