Lysosomes (tritosomes): Kulosera kwa mayeso a Vitro Form of Catabolism kapena lysosomal stabition maphunziro

Zinthu za Iphase

Mawu OyambaLysosomes
Mayshome adapezeka ndi Dea Custive mu 1950s ndikukhazikitsa ngati chapakati pa chowonongeka ndi kagayidwe ka mule. Lysosomes ndi amodzi - membrane, wamphamvu, heruogeneous organelles omwe amasiyana m'malo, Morphology, kukula, enzymes, ndi miyala ina. Nembanemba ya lysosomal ili ndi mapuloteni ambiri otumphukira, kuphatikizapo osiyanasiyanaKupititsandi ma iyoni. Makina a lysosomal - Sumsunit - ATPASE imasunga acidic lysosomal lumen. Izi Ph (4.5 - 5.5) Zimayambitsa> lysosomal ma hydroses omwe amagaya macromobolelules kuphatikiza mapuloteni, acid acids, milidshydrates. Malysomes amalandila ma molekyulu ang'onoang'ono ndi zida zowonjezera, kupfulating'ono tinthu tating'onoting'ono tomwe timawonongeka. Chifukwa chake, malysomes akhala akuona kuti "BEBCCY 'ya Bin" ya Cell.

Ma tritosome

Ma tritosomes amaphatikizika mwapadera magulu osiyanasiyana am'madzi, makamaka pakuwongolera njira za celebolic ndi kukonza holostasis. Ma tritosomes nthawi zambiri amaphunziridwa munthawi ya ntchito zawo mkati mwazinthu zachilengedwe. Mbali yovomerezeka ya izi ndi mawonekedwe awo apadera, omwe amawalola kuti azigwira ntchito zovuta kwambiri zomwe ndizofunikira kwambiri kuti mupulumuke komanso kugwira ntchito koyenera kwa maselo. Rat chiwindi ma tritosomes ali hepatic lysosomes zomwe zadzaza ndi tyloxapol (triton fl 1339), osakhala - iyonic magaructicntint. Ma Lysosomes okhala ndi Tylosapol Chiwonetsero cha Tylosapol chimachepetsa, amatha kukhala otalikirana kwambiri ndi Mitochondria, ndi kuipitsa organelles omwe amakulirapo ndi mafupa achilengedwe.

Kugwiritsa ntchito lysosomes
Mankhwala a acid a acid a acid ndi lysosomes
Mankhwala ochepa anyukiliya amatchula zidutswa zazing'ono za ma nucleotides okhala ndi madera ena omwe angamangire ku Arrnas ndikusokoneza matembenuzidwe a Mernas kuti akwaniritse zochizira. Mankhwala anti a acid anyukilimu amaphatikizapo antishongo (asos), RATANA yaying'ono (sirna), RATANA), ASNA)

Pambuyo makonzedwe, mankhwala ochepa a nuclectic acid amafunika kupewa kuwonongeka ndi minyewa ya m'magazi ndi ma lysosome, ndikuthana ndi ma endoplas kuti akwaniritse mankhwala. Lysosomes angagwiritsidwe ntchito ngati dongosolo loyenerera bwino kuti muchepetse kusintha kwa mankhwala osinthika a Nyulro, kupereka chithandizo cha data ku Vitro, kupereka chithandizo chambiri pakufufuza kwa acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a acid a ma acid a ma acid a ma acid a acid a ma acid a ma acid a ma acid a ma acid a ma acid a ma acid a acid a ma acid a ma acid a ma acid a zidziwitso zazing'ono.

·Ntibod · antibod - conjugate (ADC) ndi Lysosomes
Antibody - mankhwala conjugate (ADC) ndi mtundu watsopano wa mankhwala a biotechnology ang'onoang'ono amapanga ma antibodies kapena zidutswa za antibod kudzera ma ansala. Imatha kukulitsa kugwiritsa ntchito mankhwala osokoneza bongo komanso kukhazikika, kuchepetsa zovuta zamankhwala komanso zotsatira zoyipa, ndikusintha njira zochizira. Zonsezi zili ndi vuto la mankhwala ang'onoang'ono a molekyulu komanso kunyamula mankhwala a antibod. Amagwiritsidwa ntchito makamaka mu chithandizo cha anti - chotupa kapena matenda ena.

Mukalowa mthupi, mamolekyu a ADC amatha kumangidwa kwa ma arnigns pamwamba pazida zomwe tikupanga kudzera pa chitsogozo cha ma antibododies, ndikusamutsanso m'maselo aja. Mamolekyu a ADC omwe amalowa maselo (makamaka mu lysosomes) amatha kumasula ma bolekyu ang'onoang'ono (I.EXINE ORTUGS) ADC imafunikira ma lysosomes a ma lysosomes kuti muwongolere ndi kutulutsa mankhwala azowonjezera Mu zoyeserera za Vitro za ADC ndi Lysosomes zimatha kuwunika ngati a Lindosomes amasulidwa ndi mankhwalawa a Molek

Za Iphase
Monga wotsogolera ku Vitro Biorment Kukula kwa mankhwala, Iphase adakhazikitsa chiwindi ysosome zopangidwa ndi mitundu isanu, kuphatikizapo gulu, rat ndi mbewa zopangira mankhwala osokoneza bongo.
Ntchito ya enzyme ya enzyme: Iphase chiwindi adayesedwa kuti Cathepsin B ndi Acid Phosphatase Ntchito Yofananirako, ndipo ntchito ya enzyme imafanana ndi zinthu zina zomwezi.
Ntchito zopanga: Kupanga kwa Batch kukhazikitsidwa, ndipo kufufuza ndikokwanira kuwonetsetsa kuti ndalama zomwezo.
Kafukufuku wopititsa ndi nthawi yochepa: Kupangana, malo osungiramo zinthu zingapo m'matumba, kuti atsimikizire kuti makasitomala amagwiritsa ntchito.