Mawu osakira:Organoid, 3D Kulima, 3D Spheroid Culture, 3D cell culture, Primary Hepatocytes, Primary human hepatocytes, Primary nyani hepatocytes, Primary galu hepatocytes, Primary rat hepatocytes, Primary mouse hepatocytes, Ultra-low attachment culture plate

IPHASE Products

Mawu Oyamba

Kusinthika kwachangu kwaukadaulo wa Organoid ndi 3D cell culture kukusintha kafukufuku woyambirira. Monga makampani opanga mankhwala akufuna kulosera zambiri muvitro models, chikhalidwe cha ma cell achikhalidwe chachiwiri-dimensional (2D) chikusinthidwa m'malo ndi machitidwe olima a 3D.

Chochitika chofunikira kwambiri chidachitika mu Epulo 2025 pomwe US FDA idalengeza mapulani ochepetsa zofunikira zoyezetsa nyama pakuwunika kwa chitetezo cha monoclonal antibody pomwe encokulimbikitsa kukhazikitsidwa kwa New Approach Methodology (NAMs), kuphatikiza ma organoids ndi ma cell apamwamba amunthu - Kusintha kwa mfundozi kukuwonetsa kufunikira kwakukula kwa anthu-makina ofunikira mu vitro pakupanga mankhwala.

Pakati pa matekinolojewa, Primary Hepatocyte 3D Spheroid Culture yakhala imodzi mwazinthu zothandiza kwambiri komanso zovomerezeka kwambiri zachiwindi. Mwa kulola ma hepatocyte oyambirira kuti adzipange okha-kusonkhanitsa mu atatu-dimensional spheroids, ochita kafukufuku amatha kusunga chiwindi-zintchito zapadera kwa nthawi yaitali pamene akupeza zambiri zolosera za kagayidwe ka mankhwala, hepatotoxicity, ndi kafukufuku wa matenda a chiwindi.

Kodi Chiwindi Organoids ndi Hepatocyte Spheroids Ndi Chiyani?

Organoids ndi atatu-mawonekedwe amitundu yambiri yochokera ku ma cell tsinde omwe amabwezeretsanso kamangidwe kake ndi magwiridwe antchito a ziwalo za mbadwa. Ma organoids a chiwindi awonetsa kuthekera kwakukulu mumankhwala obwezeretsanso, kutengera matenda, komanso chithandizo chamunthu payekha.

Poyerekeza ndi stem cell-ochokera ku organoids, primary hepatocyte spheroids imayimira njira yosavuta komanso yopangika kwambiri ya chikhalidwe cha 3D spheroid. M'malo mofuna matrices ovuta a extracellular kapena maprotocol osiyanitsa, ma hepatocyte atsopano omwe amadzipatula okha amangokhalira kuphatikizira pansi pa ultra-otsika ophatikizana kuti apange compact multicellular spheroids pamene akusunga zofunikira za hepatic phenotypes.

Njira yowongokayi yapangitsa kuti hepatocyte spheroids ikhale imodzi mwachiwindi chogwiritsidwa ntchito kwambiri pa kafukufuku wamankhwala.

Chifukwa Chake Chikhalidwe Cha 3D Chimaposa Chikhalidwe Chamakono cha 2D

Ma hepatocyte oyambirira amataya msanga ma phenotype awo pamene amakula ngati ma monolayers ochiritsira. M'masiku ochepa okha, chiwindi-ntchito zinazake zimachepa kwambiri, kuphatikizapo: Cytochrome P450 enzyme,Albumin secretion,Bile acid metabolism, Drug transporter expression,Cellular polarity. Zolepheretsa izi zimachepetsa mtengo wolosera zachikhalidwe cha 2D pamaphunziro anthawi yayitali - kawopsedwe.

Mosiyana ndi izi, chikhalidwe cha 3D spheroid chimapanganso ma cell ofunika - kulumikizana kwa ma cell ndi kamangidwe kakang'ono kachilengedwe komwe kamapezeka m'chiwindi chachilengedwe. Ma spheroids omwe amabwera chifukwa cha izi amawonetsa kufunikira kwa thupi kudzera pakulumikizana bwino kwa ma cell, ma gradient a oxygen, kufalikira kwa michere, komanso kukonza kwa hepatic polarization.

Chifukwa chake, ma hepatocyte spheroids oyambira amakhalabe okhazikika pachiwindi kwa milungu ingapo m'malo mwa masiku, kuwapangitsa kukhala ofunikira kwambiri pamaphunziro okhudzana ndi mankhwala osokoneza bongo.

Ubwino wa Primary Hepatocyte 3D Spheroid Culture

1. Yaitali-Kukhazikika Kogwira Ntchito Kwanthawi yayitali

Ubwino waukulu wa chikhalidwe cha 3D primary hepatocyte spheroid ndi kusunga kwanthawi yaitali kwa chiwindi-zintchito zinazake. Polimbikitsa kuyanjana kwa ma cell-maselo ndikukonzanso chilengedwe chogwirizana kwambiri ndi thupi, ma spheroid amakhalabe ndi mawonekedwe ovuta a chiwindi omwe amatayika mwachangu m'zikhalidwe zodziwika bwino za 2D. Pansi pamikhalidwe yabwino, ma hepatocyte spheroids oyambirira amakhalabe okhazikika kupanga albumin, ntchito ya CYP450 enzyme, Phase I ndi Phase II metabolism, ndi mawu onyamula mankhwala kwa masiku oposa 21. Kutalika kwa nthawi yogwira ntchito kumeneku kumapangitsa kuti anthu azilosera kwambiri mu vitro kagayidwe ka mankhwala, kuwunika kwa hepatotoxicity, komanso kafukufuku wa matenda a chiwindi.

2. Kupititsa patsogolo Kufunika Kwathupi

Ubwino umodzi waukulu wa chikhalidwe cha 3D hepatocyte spheroid ndi kuthekera kwake kukonzanso mawonekedwe achiwindi chachilengedwe. Kupyolera mu kuchuluka kwa maselo - kuyanjana kwa maselo ndi mapangidwe a minofu-monga atatu-mapangidwe a dimensional, ma spheroids amakhazikitsa mpweya wokhudzana ndi thupi ndi michere ya michere pamene akusunga polarity ya chiwindi ndi ntchito zama cell. Zotsatira zake, ma hepatocytes oyambirira amasonyeza ntchito za kagayidwe kachakudya zomwe zimafanana kwambiri ndi zomwe zimawonedwa mu vivo, zomwe zimapangitsa kuti pakhale kuwonetseredwa bwino kwa kagayidwe ka mankhwala, ntchito ya chiwindi, ndi mankhwala - kuvulala kwa chiwindi (DILI) poyerekeza ndi zikhalidwe za 2D monolayer.

3. Mapulogalamu mu Drug Discovery ndi Chiwindi Research

Kukhazikika kwakuthupi komanso kukhazikika kwanthawi yayitali kwa Primary Hepatocyte spheroids kwawapanga kukhala zida zamtengo wapatali pamitundu yambiri yofufuza zamankhwala. Amagwiritsidwa ntchito kwambiri pamankhwala a metabolism ndi maphunziro a pharmacokinetic, kuyesa kwa hepatotoxicity, kuneneratu kwa DILI, CYP induction and inhibition assays, ndi kuyesa kukhazikika kwa metabolic. Kuphatikiza apo, hepatocyte spheroids imapereka zitsanzo zamphamvu zofufuza matenda a chiwindi, kuphatikizapo non-alcoholic steatohepatitis (NASH), chiwindi fibrosis, ndi viral hepatitis. Kuthekera kwawo kukonzanso bwino chiwindi chachilengedwe kumawapangitsanso kukhala oyenera kwambiri pamankhwala olondola, mankhwala obwezeretsa, ndi ntchito zina zapamwamba zomasulira.

Kumanga Odalirika 3D Hepatocyte Spheroids

Chikhalidwe chopambana cha 3D spheroid chimadalira zigawo zitatu zofunika:

1. High-quality Primary Hepatocytes

Maonekedwe a cell amatsimikizira mwachindunji kapangidwe ka spheroid, kutheka, ndi ntchito yanthawi yayitali.

IPHASE imapereka high-viability cryopreserved primary hepatocytes kuchokera ku mitundu ingapo, kuphatikizapo:

• Ma Hepatocytes a Anthu Oyambirira
• Ma hepatocytes a Monkey Primary
• Ma hepatocyte agalu oyamba
• Ma hepatocytes a makoswe oyambirira
• Ma hepatocytes a Mouse Primary

Ma hepatocyte onse amawongolera mosamalitsa kuti awonetsetse kuti akugwira ntchito bwino, akugwira ntchito mosasinthasintha, ndi batch-to-batch reproducibility.

2. Wokometsedwa 3D Culture Media

Makanema apadera ndi ofunika pakupanga ma spheroid komanso kukonza kwanthawi yayitali.

Ntchito ya IPHASE ikuphatikiza:

• Hepatocyte Thaw Medium
• Hepatocyte 3D Spheroid Formation Medium
• Hepatocyte 3D Spheroid Maintenance Medium

Ma media awa amathandizira kuphatikizika bwino ndikusunga magwiridwe antchito a chiwindi munthawi yonse yachikhalidwe.

3. Ultra-Mimbale Yotsika Yophatikiza Chikhalidwe

Mapangidwe odalirika a spheroid amafuna ziwiya zachikhalidwe zomwe zimalepheretsa kumamatira kwa ma cell.

IPHASE Ultra-Low Attachment Culture Plates imathandizira ma hepatocyte kuti adziphatikize okha - kusonkhanitsa ma spheroids ofananirako popanda kufunikira zokutira zakunja kwa matrix, zomwe zimapereka kusasinthika kwamayendedwe anthawi zonse a labotale.

Kutsimikizira Kwakuyesera

Pogwiritsa ntchito cynomolgus monkey primary hepatocytes, IPHASE idakhazikitsa bwino chiwindi cha 3D spheroids mkati mwa masiku asanu ndi awiri pogwiritsa ntchito chikhalidwe chake chokongoletsedwa.(Chithunzi 1 & 2).

Chithunzi 1. Woimira 3D spheroids wopangidwa kuchokera ku IPHASE Primary Cynomolgus Monkey Hepatocytes (Lot K0125E069).

Chithunzi 2. Woimira 3D spheroids wopangidwa kuchokera ku IPHASE Primary Cynomolgus Monkey Hepatocytes (Lot K0124E022).

Kuti apitirize kutsimikizira ntchito ya IPHASE 3D Primary Hepatocyte Spheroid Culture System, hepatocytes oyambirira kuchokera ku mitundu yambiri yamitundu yambiri adalimbikitsidwa pogwiritsa ntchito kayendedwe kameneka, kuphatikizapo cynomolgus monkey (Lot K0125E086), beagle dog (Lot B0125H018), ndi Sprague - Dawtesley rat hepato. Kutulutsa kwa Albumin mu chikhalidwe chapamwamba kunayesedwa pambuyo pa masiku 7 a chikhalidwe cha spheroid monga chizindikiro cha ntchito ya chiwindi (Chithunzi 1). Mitundu itatu yonseyi idapanga bwino ma spheroids okhazikika ndikusungabe kupangika kwa albumin, kuwonetsa kulimba ndi kupangika kwa nsanja ya chikhalidwe ya IPHASE 3D pamitundu yosiyanasiyana ya nyama.

Kuti muwone kukhazikika kwa nthawi yayitali kwa ma spheroids, ma cynomolgus monkey hepatocytes (Loti K0124E022) adakulitsidwa mpaka masiku 21. Kutulutsa kwa Albumin kunayesedwa nthawi zambiri nthawi yonse ya chikhalidwe (Chithunzi 2). Kupanga kosalekeza kwa albumin pa nthawi yayitali-chikhalidwe cha nthawi yaitali kumasonyeza kuti IPHASE 3D spheroid system imateteza bwino chiwindi-zintchito zapadera, kuthandizira kugwiritsidwa ntchito kwake mu maphunziro a kawopsedwe osatha, kagayidwe ka mankhwala, ndi matenda a chiwindi a nthawi yaitali.

Chithunzi 1. Kutulutsidwa kwa Albumin ndi 3D spheroids wopangidwa kuchokera ku hepatocytes oyambirira a mitundu yosiyanasiyana pambuyo pa masiku 7 a chikhalidwe.
Chithunzi 2. Kutulutsidwa kwa Albumin ndi cynomolgus monkey primary hepatocyte spheroids otukuka kwa 7 ndi 21 masiku.
IPHASE Complete Solution ya 3D Liver Models

Kuthandizira kufunikira kwakukula kwa chikhalidwe chapamwamba cha 3D cell komanso kafukufuku wa Organoid, IPHASE imapereka mayendedwe ophatikizika a chikhalidwe cha hepatocyte spheroid, kuphatikiza:

• Cryopreserved Primary Human Hepatocytes
• Ma hepatocytes a Monkey Primary
• Ma hepatocyte agalu oyamba
• Ma hepatocytes a makoswe oyambirira
• Ma hepatocytes a Mouse Primary
• Hepatocyte Thaw Medium
• Hepatocyte 3D Spheroid Formation Medium
• Hepatocyte 3D Spheroid Maintenance Medium
• Ultra-Mimbale Yotsika Yophatikiza Chikhalidwe

Zogulitsazi zimathandizira ofufuza kuti akhazikitse zitsanzo zachiwindi zolimba komanso zotha kuberekanso kuti apeze mankhwala, toxicology, kutengera matenda, komanso kafukufuku womasulira.

Mapeto

Pamene mabungwe olamulira akuchulukirachulukira kulimbikitsa anthu - njira zoyezera zoyenera, 3D Primary Hepatocyte Spheroid Culture ikukhala nsanja yofunikira kwambiri pakufufuza kwachiwindi kwamakono.

Mwa kusunga hepatic phenotype, kuwongolera kufunikira kwa thupi, ndikuthandizira maphunziro anthawi yayitali, ma hepatocyte spheroids amatsekereza kusiyana pakati pa chikhalidwe cha cell ndi mu vivo biology.

Ndi apamwamba-akuluakulu a Hepatocytes, chikhalidwe chokongoletsedwa bwino, ndi makina apadera a Ultra-Low Attachment Culture Plates, IPHASE imapatsa ochita kafukufuku yankho lathunthu pomanga zitsanzo zachiwindi za 3D zomwe zimafulumizitsa kupeza mankhwala ndi kafukufuku wamankhwala.