Mhedzisiro isingagutsi bvunzo? Iyo vhezheni itsva yeiyo - vitro micronuclcleus bvunzo kit iri pano kubatsira!

Chikamu 01. In - Vitro Micronuchcleus bvunzo

Microniclei yakazara chromatids kana zvigadzirwa zvechirume kana yeiyo chromosomes iyo inoramba iri muCytoplasm iyo chromosomes inogara ichipinda mwanasikana kuti aumbe nuclei mushure memagosis. Nekuti zvidimbu izvi kana chromosomes hachigoni kupinda muTolous maincus, apo mwanasikana mwanasikana anopinda muparara anotevera, vanozvinyudza vhurume kunze kwenyika kunze kwenyika uye fomu micronuclei. Nekurereka kweiyo fomu yekufananidza, kukosha kweMicronuclei, iko kuvandudzwa kwekuona maitiro ekuona, bvunzo dzekuyedza dzakazova imwe yenzira huru dzekutarisa miteji. Inoshandiswa zvakanyanya zvekufanotaura necarcinogenic njodzi, kuona anauploidy inducer uye mamwe mafuro ane njodzi.

Iyo - Vitro Micronuchcleus bvunzo inzira yekuongorora nzira yekuona kugadzirwa kweMicronclei muMammolies maseru mushure mekurapwa nechinhu chebvunzo. Izvo zvakanakira kutarisa chromosomes kuputsa uye azouploidy inokonzerwa mumitotic masero panguva / mushure mekuratidzwa kune chinhu chebvunzo. Kuenzaniswa neiyo - Vivo bvunzo, iyo in - Vitro MicronCleus bvunzo iri nyore, nekukurumidza, uye zvakachipa. Mukuwedzera, haina kukanganiswa nezvinhu zvakadai semisiyano yemunhu pakati pemhuka dzekuedzwa. Sezvo mu - vitro mikronuclus bvunzo inogona kuona micronuchclus maitiro anokonzerwa nemamwe makemikari pane yakaderera kutarisisa nekunyanyisa kunzwa. Naizvozvo, yakashandiswa zvakanyanya mumunda we genetic ineology.


Chikamu 02. Mu vitro micronuclcleus bvunzo kit

Iyo Iphase in - Vitro MicronCleus bvunzo kit inoshandisa Chinese Hamster Lung (chl) masero seyekuedzwa system. Iyo CL CELS inofumurwa nechinhu chebvunzo ne / pasina metabolic activation system uye cell polbitor cytochalalasin b. Ipapo microncleus mwero wemasero ayo akapedza imwe mitambo yeMitosis Kithahase kit inopa zvese zvakakosha reagents uye masero e - vitro micronuclucleus kuyedza. Sezvo kits yese yakapfuura zvakasimba zvekuyedza, iyo yakaburitsa miedzo yekuedza ndeyechokwadi, yakavimbika uye inobereka.

-- Chigadzirwa Zvigadzirwa --

1. Nyore

Inochengetedza nguva yeiyo yakakonzerwa S9 uye reagent kugadzirira ,. Kit inogona kushandiswa zvakananga, kupfupikisa zvakanyanya kutenderera kwekuedza.

2. Kunyatso

Chimwe nechimwe chikamu cheiyo Reagent Kit yakaiswa kuongororwa kwakasimba kwekuongororwa, saka mhedzisiro yekuedza uchishandisa kit ndeyechokwadi, yakavimbika uye yakanyanya kubereka.

3. Kugadzikana

Iyo kit yakagadzikana uye iri nyore kutakura uye kuchengeta.

4. Kuedza

Yakawanda mhando dzekushandisa, dzinogona kushandiswa genetic kuongororwa kwechikafu, makemikari, zvipembenza, zvipuka zvezvipfava, zvinowedzera zvekudya, zvidhori, zvikafu, uye vamwe vazhinji.

Kuenzanisa kweiyo nyowani uye yekare kits

Iphase anotevera maitiro emunyika uye nhungamiro yekuva - vitro micronuchqleus bvunzo muminda dzakasiyana siyana seyakatungamira hunyanzvi. Zvakare, iyo Phaase inotora mhinduro yevatengi seyakawana chinangwa chekugadzirisa kuumbwa uye nzira yechigadzirwa uye yakabudirira kukwidziridzwa bvunzo dzekutevera kuti tisangane neakawanda zvevatengi zvinodiwa kunyangwe mushure mekurarama kuenderera mberi.

1. Chigadzirwa Chekuumbwa

Giemesa Stagents Re Reagents uye Re Re reaagens inodiwa kuti gadziriro yekugadzirira yakabatanidzwa kuti iite kuti ive nyore uye inoshanda.

2. Nzira

Iyo kit protocol yakanyanya kutsanangurwa, nekubatanidzwa kwekuongororwa kwekutarisira uye zvimwe zvirimo kuti udzivise zvikanganiso zvakajairika.

Chikamu 03. Zvigadzirwa zvine chekuita