Nsonaazụ ule na-adịghị mma? Ụdị ọhụrụ nke in- vitro micronucleus test kit dị ebe a iji nyere aka!

PART 01. Na - Vitro Micronucleus Test

Micronuclei bụ chromatids dum ma ọ bụ iberibe acentric ma ọ bụ chromosomes mgbanaka nke na-anọgide na cytoplasm mgbe chromosomes na-abanye n'ime sel nwa nwanyị na-etolite nuclei mgbe mitosis gasịrị. N'ihi na mpempe akwụkwọ ndị a ma ọ bụ chromosomes enweghị ike ịbanye na isi nucleus na telophase, mgbe ụmụ nwanyị na-abanye na interphase na-esote, ha na-agbanye n'ime obere oghere n'èzí isi oghere ma na-etolite micronuclei. Site na nkọwapụta nke usoro nhazi, mkpa micronuclei pụtara, nkwalite na-aga n'ihu nke usoro nchọpụta na izu oke nke usoro nnwale, ule micronucleus ghọrọ otu n'ime ụzọ bụ isi maka mmụgharị nyocha. A na-eji ya n'ọtụtụ ebe maka ịkọ ihe ize ndụ carcinogenic, ịchọpụta ihe na-akpata aneuploidy na ihe egwu mkpụrụ ndụ ihe nketa ndị ọzọ.

Nnwale in-vitro micronucleus bụ usoro nnwale genotoxicity maka ịchọpụta mmepụta nke micronuclei n'ime mkpụrụ ndụ anụ mammali mgbe ejiri ihe nyocha mechaa ya. Ọ dị mma maka ịlele mgbaji chromosome na aneuploidy na-ebute n'ime sel mitotic n'oge/mgbe ekpughere ihe nyocha ahụ. E jiri ya tụnyere in-vivo test, in- vitro micronucleus test dị mfe, ngwa ngwa ma dị ọnụ ala. Na mgbakwunye, ọ naghị emetụta ihe ndị dị ka ọdịiche nke onye ọ bụla n'etiti anụmanụ ule. Ebe ọ bụ na in - vitro micronucleus ule nwere ike ịchọpụta mmeghachi omume micronucleus nke ụfọdụ kemịkalụ na-ebute na obere mkpokọta nwere mmetụta dị elu. Ya mere, ejirila ya mee ihe n'ọtụtụ ebe na ngalaba nke mkpụrụ ndụ ihe nketa toxicology.


PART 02. In vitro micronucleus test kit

Ngwa nyocha IPHASE in- vitro micronucleus na-eji mkpụrụ ndụ Hamster Lung China (CHL) dị ka usoro nnwale. A na-ekpughere mkpụrụ ndụ CHL na ihe nyocha na-enweghị / na-enweghị usoro mmemme nke metabolic ma na-emeso ya na actin polymerization inhibitor cytochalasin B. A na-ahapụ mkpụrụ ndụ ka ha ruo oge kachasị mma nke sel binucleated, wee wepụta ya, kwadebere, na ntụpọ; Mgbe ahụ, a na-enyocha ọnụ ọgụgụ micronucleus nke mkpụrụ ndụ nke mezuru otu mitosis (sel ndị nwere ọnụọgụgụ) n'okpuru microscope iji nyochaa ikike mutagenic nke ihe nnwale ahụ. Ngwa IPHASE na-enye reagents na sel niile dị mkpa maka nnwale in - vitro micronucleus. Ebe ọ bụ na ngwa niile agafeela ule dị mma siri ike, nsonaazụ nnwale emepụtara bụ nke ziri ezi, ntụkwasị obi yana enwere ike imepụtagharị ya.

--Uru ngwaahịa --

1. Mma

Na-echekwa oge maka mkpali S9 na reagent nkwadebe,. Enwere ike iji ngwa mee ihe ozugbo, na-ebelata usoro nnwale ahụ nke ukwuu.

2. izi ezi

Edobela akụkụ ọ bụla nke ngwa reagent nke ọma, yabụ nsonaazụ nnwale na-eji ngwa ahụ bụ nke ziri ezi, ntụkwasị obi yana enwere ike imepụtaghachi ya nke ukwuu.

3. Nkwụsi ike

Ngwa ahụ kwụsiri ike ma dị mfe ibufe na ịchekwa.

4. Ntughari

Ngwa dịgasị iche iche, enwere ike iji ya mee nyocha toxicology nke mkpụrụ ndụ ihe nketa nri, kemịkalụ, ọgwụ pesticides, ọgwụ nje, ihe mgbakwunye nri, ihe ịchọ mma, na ọtụtụ ndị ọzọ.

Ntụle nke ngwa ọhụrụ na nke ochie

IPHASE na-agbaso ụkpụrụ na ụkpụrụ mba maka in- vitro micronucleus ule n'akụkụ dị iche iche dị ka ntuziaka ọrụaka ya. Ọzọkwa, IPHASE na-ewere nzaghachi ndị ahịa dị ka ebumnobi kachasị ya iji dozie nhazi na usoro nke ngwaahịa ahụ wee kwalite ngwa nyocha in-vitro micronucleus nke ọma iji gboo mkpa ndị ahịa ọbụlagodi mgbe nkwenye na-aga n'ihu.

1. Ngwakọta ngwaahịa

Agbanyela ihe nchacha Giemsa na reagents achọrọ maka usoro nkwadebe ka etinyere ya ka ọ dabara adaba ma rụọ ọrụ nke ọma.

2. Usoro

Usoro ngwa ngwa ahụ bụ nkọwa zuru ezu karị, yana nsonye elele ule na ọdịnaya ndị ọzọ iji zere mmejọ nkịtị.

PART 03. Ngwaahịa emetụtara