Mfundo yofunika
Zithunzi za IPHASEPBMC kudzipatula zidas amachokera ku njira yachikale ya kachulukidwe kachulukidwe ka centrifugation, yomwe imachokera pa kusiyana kwa kukhazikika kwa liwiro la maselo osiyanasiyana a magazi mu kachulukidwe ka gradient kuti akwaniritse kupatukana kwa maselo. Kuphatikizidwa mu zida ndi njira yolekanitsa ya kachulukidwe kachulukidwe kake kamene kamapanga kachulukidwe kachulukidwe kokhazikika panthawi ya centrifugation. Pamene magazi a anticoagulated amawonjezeredwa pang'onopang'ono pa njira yolekanitsa, maselo ochuluka kwambiri monga maselo ofiira a magazi ndi granulocytes amakhazikika pansi pa chubu cha centrifuge, pamene PBMC yocheperako imayimitsidwa pa mawonekedwe pakati pa njira yolekanitsa ndi plasma, kupanga maselo omveka bwino, oyera, amtambo. Mwa kusonkhanitsa mosamala wosanjikiza wa ma cell ndikutsuka kotsatira ndi centrifugation, chiyero chachikulu cha PBMC chikhoza kupezeka.Chiyambi chachidule
IPHASE's PBMC Isolation Kit ndi chida chopangidwira ofufuza kuti azitha kupatula bwino PBMC ku magazi athunthu omwe ali ndi anticoagulated. Chidachi chili ndi izi:1.Kupatukana kwabwino kwambiri: kugwiritsa ntchito cholekanitsa chokhazikika cha kachulukidwe kachulukidwe kumatsimikizira kulekanitsa kofulumira komanso kolondola kwa PBMC panthawi ya centrifugation, ndikuchepetsa kuipitsidwa kwamitundu ina.
2.Kuyera kwapamwamba: kupyolera mu njira yolekanitsa bwino ndi masitepe osamba, chiyero chapamwamba cha PBMC chikhoza kupezeka, kupereka maziko odalirika a chikhalidwe cha maselo, kuyesa kwa biology ya maselo ndi zina zotero.
3. Sterility ndi endotoxin yochepa: njira yothetsera kudzipatula ndi zigawo zina mu kit zakhala zikutsatiridwa ndi mankhwala okhwima a aseptic ndi ma endotoxin otsika kwambiri, kuonetsetsa kuti palibe zonyansa zosafunikira zidzayambitsidwa panthawi yodzipatula.
4.Kugwiritsidwa ntchito kwambiri: osati koyenera kokha kwa PBMC yaumunthu kudzipatula, komanso imapereka zida zapadera zodzipatula za PBMC kwa mitundu ina ya zinyama (monga nyani, galu, makoswe, mbewa, ndi zina zotero) kuti akwaniritse zosowa zosiyanasiyana zafukufuku.
5.Easy kugwira ntchito: Chidacho chili ndi malangizo ogwiritsira ntchito mwatsatanetsatane ndi zida zofunikira zothandizira, zomwe zimapangitsa kuti njira yonse yodzipatula ikhale yosavuta komanso yosavuta, ngakhale kwa oyamba kumene kuti ayambe mwamsanga.
Zitsanzo za ntchito
Zida zodzipatula za IPHASE za PBMC zakhala zikugwiritsidwa ntchito kwambiri pofufuza zamankhwala osiyanasiyana. Mwachitsanzo, mu kafukufuku wa immunology, ofufuza angagwiritse ntchito PBMC yolekanitsidwa kuti asankhe ndi kufufuza ntchito ya maselo a T, maselo a B ndi maselo ena a chitetezo cha mthupi; mu kafukufuku oncology, PBMC angagwiritsidwe ntchito kuwunika mmene chitetezo cha m'thupi odwala chotupa ndi chophimba anti-chotupa maselo chitetezo cha m'thupi. Kuphatikiza apo, zidazi zimagwiritsidwa ntchito kwambiri popanga katemera, kuyesa mankhwala ndi zina.Mapeto
Zida zodzipatula za IPHASE za PBMC zatenga malo ofunikira kwambiri pankhani ya kafukufuku wazachipatala ndikuchita bwino kwambiri komanso kugwiritsa ntchito kwambiri. Ndi kupita patsogolo kosalekeza kwa sayansi ndi ukadaulo komanso kuchuluka kwa kafukufuku yemwe akuchulukirachulukira, IPHASE ipitiliza kudzipereka pakupanga zinthu zabwino kwambiri, zoyera komanso zosavuta-kugwiritsa ntchito zida za bioloji kuti zipereke chithandizo champhamvu kwa ofufuza.Nthawi yotumiza: 2024 - 07 - 27 16:30:36