Taw qhia rauLier microsomesthiab s9 feem
Nyob rau hauv thaj teb cov tshuaj metabolism thiab cov kws ua tshuaj, nkag siab cov lus qhia ntawm daim siab micromes thiab s9 feem yog qhov tseem ceeb. Cov hauv cov tshuab tsim khoom lag luam muaj yees hauv cov theem thaum ntxov ntawm kev pom thaum ntxov ntawm cov khoom siv tshuaj lom neeg tshiab (nces) yog metabolized los ntawm daim siab. Lub peev xwm los twv kev ruaj khov metabolic ruaj thiab muaj peev xwm kev sib cuam tshuam yog qhov tsis muaj nqis, txuag lub sijhawm thiab cov peev txheej hauv kev txhim kho cov chaw muag tshuaj tshiab. Tsab ntawv no rho tawm hauv qhov sib txawv ntawm daim siab micromomes thiab s9 feem ntawm daim duab me me, tshawb txog lawv muaj pes tsawg leeg, kev ua haujlwm zoo, thiab kev txwv.Muaj pes tsawg leeg thiab cov qauv ntawm daim siab micromomes
● microSomes ua ib feem ntawm endoplasmic reticulum
Lier microtic yog vesicle - xws li cov khoom cuav muab tau los ntawm EndoPlasmic reticulum hauv Eukaryotic hlwb. Lawv tau tsim thaum cov hlwb tau tawg thaum lub sijhawm homogenization, uas ua rau cov sedimentation ntawm endoplasmic reticulum. Qhov kev ncua sij hawm subection no tau txhawb nrog membrane -● Cov ntsiab lus tseem ceeb koom nrog: Cytochrome P450s thiab Umts
Lub siab siab ntawm Cytochrome P450 enzymes (cyps) hauv daim siab micromomes yog lawv lub ntsiab lus txhais tau. Cov enzymes no ua si lub luag haujlwm tseem ceeb hauv Oxidatative Lej ntawm oxididatism ntawm ntau cov xenobiotics, suav nrog tshuaj thiab ib puag ncig cov co toxins. Tsis tas li tam sim no yog uridine diphosphate - gluoluronfortferases (wearts), uas ua rau kev tshem tawm ntawm cov theem kuv thiab qee ntu II tawm tsam.Muaj pes tsawg leeg thiab cov qauv ntawm S9 feem
● S9 raws li kev sib xyaw ntawm microsomal thiab cytos cov leb
S9 s9 feem, feem ntau hu ua 9000 supernatant, yog ib tug subcellular tivthaiv tau los ntawm daim siab homogenates los ntawm kev sib zog ntawm centrifugation. Tsis zoo li microsomes uas muaj presominly endomasmic reticulum - bol enzymes, SYNTOSOLES thiab cytosolic enzymes, qhia ib qho kev tshaj lij enzymatic profile.● Cov enzymes tam sim no nyob rau hauv s9 suav nrog rau theem I thiab II
S9 cov zauv feem muab cov enzymatic milieu, encapsulating ob qho tib si theem kuv enzymes, xws li theem II enzymes, thiab gluathionys - s - kev hloov pauv. Cov array no tso cai rau cov kev hloov pauv metabolic ntau, simulating daim siab nyob hauv Vivo ib puag ncig ntau dua li microsomes ib leeg.Kev ua haujlwm: Theem I thiab Theem II Metabolism
● Sib txawv sib txawv ntawm enzyme kev ua ntawm micromomes thiab s9
Cov tub ntxhais sib txawv nyob hauv cov enzymatic. Liver microsomes feem ntau txhawb theem kuv metabolic cov tshuaj tiv thaiv, koom nrog oxidation, txo, thiab hydrolysis. Cov kev tawm tsam no tau qhia lossis nthuav tawm cov pab pawg ua haujlwm ntawm cov tshuaj molecules, paving txoj hauv kev rau theem II tawm tsam. Hauv kev sib piv, S9 cov zauv feem ntau ntxiv rau kuv thiab II, yog li pab txhawb cov conjugation uas microsomes tsis tuaj yeem ua haujlwm ywj siab.Qhov tseem ceeb ntawm theem I thiab II hauv cov tshuaj metabolism
Cov kev sib tw ntawm theem I thiab II tau cuam tshuam nrog lawv qhov tseem ceeb hauv cov tshuaj metabolism. Theem i hloov kho feem ntau npaj cov tebchaw rau theem II, uas feem ntau cuam tshuam nrog cov solubility, pab nyob rau hauv tus txheej txheem excretion. Nkag siab txog cov theem no yog qhov tseem ceeb rau kev ua yeeb tshuaj ntawm cov tshuaj Bioavailability, kev ua tau zoo, thiab tej zaum muaj tshuaj lom.Cov ntawv thov hauv cov kev tshawb fawb tshuaj yeeb
● Siv hauv kev tshawb fawb hauv kev tshawb fawb: kev nqus, faib cov khoom siv, cov metabolism, tshem tawm
Lier micraphomes thiab s9 feem yog pivotal hauv kev tshawb fawb hauv kev xav, uas ntsuas qhov nqus, cov metabolism, thiab tua cov tshuaj sib tw. Cov kev tshawb fawb no yog qhov tseem ceeb hauv kev txiav txim siab cov tshuaj, muab cov ntaub ntawv tseem ceeb uas qhia txog kev sib cuam tshuam, thiab kev mob tshwm sim.Kev Tshawb Nrhiav Kev Kawm Sib Ntsig: Microsomes vs. S9 hauv kev tshuaj pom tshuaj
Kev txheeb xyuas ntawm microsomes thiab s9 feem qhia tias thaum ob qho kev siv, S9 feem kev pom ntawm kev sib tw metabolic stability vim tias muaj kev suav nrog theem II enzymes. Qhov kev ntsuas kev tsom xam txhua qhov hauv kev txheeb xyuas cov pathwing ntawm cov pathweS uas yuav tsis pom meej thaum siv daim tawv micromoma ib leeg.Qhov zoo ntawm kev siab ntsuas microsomes
Tus nqi - Cov txiaj ntsig zoo thiab muaj
Lier microsomes tau zoo rau lawv tus nqi - Cov hauj lwm tau zoo thiab muaj kev tiv thaiv ntau yam. Cov txheej txheem rau lawv cov kev npaj thiab siv yog zoo - tsim, ua rau lawv xaiv rau ntau lub chaw soj ntsuam.● Siab siab thiab automation tau
Qhov yooj yim ntawm lub microshome cov yeeb yam ua rau lawv muaj kev sib cav siab thiab kev siv tshuab siab thiab kev ua haujlwm tseem ceeb rau kev tshuaj ntsuam cov ntawv loj ntawm cov teb chaws sai. Qhov txiaj ntsig no yog qhov tseem ceeb hauv kwj dej kev tshawb pom kev tshuaj tau pipeline, pub cim ntxov ntawm kev cog lus ntawm cov neeg sib tw.Qhov zoo ntawm daim siab s9 feem
● Nkag mus rau cov neeg metabolic
Nrog rau ob theem i thiab II enzymes, s9 cov leb muaj cov kev ntsuam xyuas me ntsis ntawm kev sib txuas ua ke metabolic. Qhov kev sawv cev thoob plaws yog muaj txiaj ntsig tshwj xeeb rau kev txheeb xyuas cov peev xwm uas muaj peev xwm ua tau thaum ntxov hauv kev tsim tshuaj.● Inlusion of Pase II metabolism thiab kev txiav txim siab nqi
Xws li cov theem II enzymes txhais tau tias S9 cov feem muaj peev xwm muab cov kev pom rau cov tshuaj yuav ua li cas thiaj li yuav ua tiav tshuaj. Thaum lawv yuav xav tau cov cofactors ntxiv rau qee yam kev tawm tsam, lawv tseem muaj kev nyiam sib npaug ntawm tus nqi thiab kev siv hluav taws xob hauv kev nce siab.Cov kev txwv ntawm Daim Ntawv Kho Mob Mas Hlis
● Tsis muaj ntawm cytosolic enzymes cuam tshuam rau cov ntaub ntawv metabolism tag nrho
Qhov tseem ceeb ntawm daim siab micromomes yog lawv tsis muaj cytosolic enzymes, uas yog qhov tseem ceeb rau daim duab metabolic. Qhov kev qhaj no txhais tau tias qee cov metabolites yuav raug saib dhau los, ploj mus txoj hauv kev tseem ceeb rau kev nkag siab txog kev siv yeeb tshuaj thiab kev ua tau zoo.● Cov tseev kom muaj rau theem II kawm
Txhawm rau kawm theem II kev tawm tsam tau txiav txim siab ntxiv, ntxiv nrog cov enzymes sab nraud / cofactors yog qhov tsim nyog thaum siv microsomes. Qhov kev xav tau no tuaj yeem ua kom tiav cov qauv zoo nkauj thiab kev txhais.Txwv ntawm daim siab s9 feem
Xav tau CO - Txheej txheem thiab ua tau enzyme dilution
Txawm hais tias lawv muaj feem ntau, cov feem ntau yuav tsum tau ntxiv ntawm cofactors los pab txhawb qee yam kev ua si enzymatic. Qhov kev xav tau no tuaj yeem qhia txog kev hloov pauv hloov pauv thiab cov nyom rau hauv cov kev cia siab. Ib qho ntxiv, cov dilution ntawm cov enzymes hauv s9 feem, piv rau kev npaj cais, tuaj yeem cuam tshuam cov rhiab ntawm kev pom rau qee cov kev siv metabolic.● Cia thiab npaj cov kev sib tw rau microsomes
Kev npaj thiab khaws cia ntawm s9 cov leb feem yuav nyuaj dua rau microsomes. Kev saib xyuas kom zoo ntawm cov kev ua yeeb yam los ntawm kev ceev faj thiab kev khaws cia yog qhov tseem ceeb kom tau txais cov txiaj ntsig txhim khu kev qha, kev tshawb fawb me ntsis.Xaus thiab lus pom zoo
● Cov ntsiab lus ntawm qhov zoo tshaj - Siv cov xwm txheej rau txhua txoj kev
Liver micromomes thiab s9 feem ntau nyias muaj qhov sib txawv thiab tsis muaj zog. Lier micromomes zoo tshaj plaws thaum raug nqi - Ua tau zoo, theem nrawm Kuv kuaj tau. Hauv kev sib piv, s9 cov lev yog qhov zoo dua rau cov kev tshawb fawb uas xav tau hauv theem I thiab II metabolism. Xaiv cov txheej txheem tsim nyog nyob ntawm qhov tshwj xeeb ntawm cov kev tshawb fawb thiab cov peev txheej muaj.● Cov lus qhia rau kev siv zog siv hauv cov txheej txheem kev tsim tshuaj
Rau cov neeg nyob rau theem kev tsim tshuaj muaj kev nyab xeeb, ua haujlwm siab dua daim siab micrutable daim ntawv tsav tsheb tuaj yeem tsim cov kev kuaj mob ntawm kev pib. Raws li kev tshawb fawb nce zuj zus, sib txuamMob siab s9Cov feem ua cov feem, tej zaum yuav tau txais los ntawm lub siab kom haum rau microster ntawm kev sib tw metabolic, kom pom kev sib tw ntawm cov yeeb tshuaj.● Qhia rauAthe
Lub taub hau Wales, Pennsylvania, Ipase Biosciences yog "siab tshaj plaws, kev tsim khoom, kev lag luam ntawm cov tshuaj lom neeg roj ntsha tshiab. Leveraging cov txuj ci zoo heev thiab mob siab rau kev tshawb fawb, peb pab neeg tshawb fawb tau cog lus thoob plaws lawv cov hom phiaj kev tshawb fawb kom ua tiav lawv cov hom phiaj tshawb fawb. Kev nrhiav tus R & D zoo tagnrho ntawm "kev tsim tawm tshiab, tshawb nrhiav yav tom ntej," chaw muag khoom, chaw muag khoom, thiab cov neeg faib khoom lag luam thoob plaws ntiaj teb.
Lub Sijhawm Post: 2024 - 11 - 26 - 26 16:02