Vim li cas NOD SCID Mouse Plasma yog qhov tseem ceeb rau Immunodeficient Model - Raws li Kev Tshawb Fawb Preclinical

Ntsiab lus:NOD SCID nas, NOD SCID nas ntshav plasma, dawb paug matrix, immunodeficient nas, Bioanalysis, txoj kev loj hlob, pharmacokinetics, kev tshawb fawb preclinical, Analytical Method Development

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Taw qhia

NOD SCID mdej khov yog ib qho ntawm feem ntau siv immunodeficient nas lim hauvkev tshawb fawb preclinical. Cov nas no muaj kev hloov pauv hauv lubPrkdcCov noob, uas ua rau muaj kev tiv thaiv kab mob hnyav heev thiab tsis ua haujlwm T thiab B lymphocytes. Tsis tas li ntawd, NOD keeb kwm keeb kwm yav dhau muaj qhov siab - affinity Sirpα allele, uas khi rau tib neeg CD47 ua tau zoo los inhibit macrophage phagocytosis ntawm tib neeg lub hlwb, thiab tseem ua rau cov ntshav tsis muaj macrophage - cuam tshuam txog endogenous cuam tshuam uas tshwm sim hauv plasma los ntawm lwm yam kev tiv thaiv kab mob nas.

Vim yog cov yam ntxwv no, NOD SCID nas feem ntau siv rau hauvkev tshawb fawb xenograft, kev hloov pauv kev tshawb fawb, thiab humanized nas qauv kev loj hlob. Lawv lub cev tiv thaiv kab mob siab heev ua rau lawv tshwj xeeb tshaj yog muaj txiaj ntsig zoo rau kev sim uas yuav tsum tau ua kom muaj sia nyob lossis muaj sia nyob ntawm cov hlwb txawv teb chaws. Hauv cov ntsiab lus no, cov khoom siv roj ntsha tau muab los ntawm NOD SCID nas, suav nrog ntshav ntshav, tuaj yeem ua lub luag haujlwm tseem ceeb hauv kev txhim kho kev soj ntsuam thiab kev tshuaj xyuas ua ntej.

Vim li cas NOD SCID Mouse Plasma tseem ceeb

Hauv bioanalytical workflows, kev xaiv ntawm matrix tuaj yeem cuam tshuam qhov kev ntsuas kev ua tau zoo. Plasma tsis yog ib hom qauv passive xwb; nws yog ib feem ntawm qhov kev tshuaj ntsuam xyuas thiab tuaj yeem cuam tshuam kev rov qab los, cov teebmeem matrix, precision, thiab kev soj ntsuam tag nrho. Rau kev tshawb fawb tsim nyob ib ncig ntawm NOD SCID qauv, sivNOD SCID nas plasma as adawb matrix tuaj yeem pab xyuas kom meej tias cov qauv, kev tswj hwm, thiab cov qauv kev tshawb fawb tau ua raws li keeb kwm ntawm kev sim tshuaj lom neeg.

Tsis zoo li cov plasma los ntawm cov nas uas muaj kev tiv thaiv kab mob ib txwm muaj (xws li BALB / c, C57BL / 6), NOD SCID nas plasma muaj qis heev ntawm endogenous immunoglobulins, ntxiv cov khoom, thiab kev tiv thaiv kab mob - ntsig txog cytokines, uas tuaj yeem txo qhov tsis yog - tshwj xeeb khi, matrix cuam tshuam rau MS thiab MS. kuaj pom.

Rau cov kev tshawb fawb raws li NOD SCID qauv, hom kab no - sib phim matrix tuaj yeem zam kom tsis txhob muaj qhov yuam kev uas tshwm sim los ntawm kev tsis sib haum xeeb keeb kwm ntawm cov qauv calibration thiab cov qauv kawm tiag tiag.

Cov ntawv thov siv

NOD SCID nas plasma yog nquag siv nyob rau hauv ntau yam kev tshawb fawb kev tshawb fawb. Ib qho ntawm nws cov kev siv ntau tshaj plaws yog raws li ib qho dawb paug matrix rau quantitativebioanalysis. Hauv lub luag haujlwm no, nws tuaj yeem siv los npaj cov qauv ntsuas ntsuas thiab cov qauv kev tswj xyuas zoo rau LC - MS / MS, immunoassay, lossis lwm lub platforms ntau.

Nws kuj tseem siv tau hauvtxoj kev txhim kho, qhov twg cov kws tshawb fawb yuav tsum tau soj ntsuam seb qhov kev ntsuam xyuas puas tuaj yeem ntsuas qhov tseeb thiab tsis tu ncua ntawm cov tshuaj ntsuam xyuas hauv keeb kwm keeb kwm lom neeg. Thaum lub sijhawm no, cov teebmeem matrix, kev rov qab los, thiab kev rov ua dua qub yog cov ntsiab lus tseem ceeb ntawm kev ntsuas.

Lwm daim ntawv thov tseem ceeb yog nyob rau hauvKev tshawb fawb pharmacokinetic (PK).. Txij li thaum PK tsom xam nyob ntawm qhov zoo ntawm cov khoom siv roj ntsha zoo, cov qauv ntshav zoo ib yam thiab tsim nyog yog qhov tseem ceeb rau kom tau txais cov ntsiab lus tseem ceeb - cov ntaub ntawv sijhawm. Hauv cov kev tshawb fawb uas muaj NOD SCID nas, siv tib hom kab mob - plasma keeb kwm yav dhau tuaj yeem txhim kho kev txheeb xyuas qhov sib xws thiab pab txo qis kev sib txawv.

Tsis tas li ntawd, NOD SCID nas plasma tej zaum yuav muaj txiaj ntsig hauv kev tshawb fawb kev txhais lus, tshwj xeeb hauv kev tshawb fawb txog oncology, kev kho ntawm tes, thiab kev tiv thaiv kab mob - ntsig txog qauv. Raws li ntau txoj haujlwm preclinical vam khom rau cov kab mob tiv thaiv kab mob, qhov kev xav tau rau cov kab mob txhim khu kev qha - cov ntaub ntawv lom neeg txuas ntxiv mus ntxiv.

Kev txiav txim siab rau Sample Handling

Raws li nrog rau txhua yam kev lom neeg matrix, kev tuav kom zoo yog qhov tseem ceeb los khaws cov qauv kev ncaj ncees. NOD SCID nas plasma yuav tsum tau muab khaws cia thiab ua tiav raws li kev tswj xyuas kom txo qis degradation thiab variability. Rov qab khov-thaw cycles yuav tsum tau zam thaum twg los tau, vim hais tias lawv yuav cuam tshuam cov qauv zoo thiab analytical reproducibility.

Nws tseem yog ib qho tseem ceeb kom khaws cia thiab ua cov xwm txheej zoo ib yam ntawm cov khoom siv. Cov yam ntxwv xws li hom tshuaj tiv thaiv coagulant, centrifugation tej yam kev mob, cia kub, thiab thawing txheej txheem tuaj yeem cuam tshuam qhov kawg ntawm cov ntshav plasma. Rau txoj kev txhim kho thiab kev siv tau, qhov sib xws hauv cov kev hloov pauv no feem ntau tsuas yog qhov tseem ceeb xws li cov khoom siv roj ntsha nws tus kheej.

Cov kws tshawb fawb yuav tsum tau ua tib zoo saib xyuas cov qauv zoo. Hemolyzed, lipemic, lossis lwm yam kev cuam tshuam cov ntshav plasma tuaj yeem qhia qhov cuam tshuam tsis tsim nyog rau hauv qhov kev ntsuam xyuas thiab yuav tsum tau soj ntsuam kom zoo ua ntej siv.

Cov txiaj ntsig rau Assay Development

Siv tus qauv - sib phim plasma matrix tuaj yeem ua kom yooj yim txhim kho kev soj ntsuam ntau txoj hauv kev. Ua ntej, nws muab cov keeb kwm yav dhau los cuam tshuam ntau dua rau kev ntsuas tus cwj pwm ntsuas hauv qhov kev sim xav. Qhov thib ob, nws tso cai rau cov kws tshawb fawb los txheeb xyuas thiab tswj cov matrix - ntsig txog cov teeb meem ua ntej hauv kev ua haujlwm. Thib peb, nws txhim kho kev sib piv ntawm cov qauv, kev tswj hwm, thiab cov qauv kawm.

Rau cov chaw soj nstuam tsim cov kev ntsuam xyuas rau cov qauv kev tiv thaiv kab mob, NOD SCID nas ntshav ntshav tuaj yeem ua tau tshwj xeeb vim tias nws txhawb nqa qhov kev txheeb xyuas qhov tseeb dua. Qhov no yog qhov muaj txiaj ntsig tshwj xeeb rau cov kev tshawb fawb uas qhov kev ntsuam xyuas kev ua tau zoo yuav tsum nyob ruaj khov nyob hauv ntau qhov kev khiav lossis hla ntau cov qauv sib txawv.

Hauv kev tswj hwm lossis ib nrab - tswj kev tshawb fawb chaw, kev tsim tawm thiab kev taug qab kuj tseem ceeb. Lub qhov dej zoo - yam ntxwv plasma matrix tuaj yeem ua rau cov ntaub ntawv huv huv thiab cov txheej txheem siv tau yooj yim dua.

Kev Tshawb Fawb Tshaj Tawm

Tshaj li niaj hnub bioanalysis, NOD SCID nas plasma kuj tseem muaj feem cuam tshuam rau ntau yam kev siv tshuaj kho mob. Raws li cov qauv kev tiv thaiv kab mob txuas ntxiv siv rau hauv oncology, kev tiv thaiv kab mob, thiab kev txhim kho kev kho mob siab heev, strain - tshwj xeeb lom matrices tau dhau los ua qhov tseem ceeb ntxiv rau kev txhawb nqa kev ua haujlwm hauv qab.

Piv txwv li, hauv kev kho ntawm tes los yog kev tshawb fawb ntawm tib neeg, cov kws tshawb fawb yuav xav tau lub plasma matrix uas sib haum nrog cov qauv keeb kwm yav dhau thiab cov kev tshuaj ntsuam xyuas. Hauv cov xwm txheej no, kev siv cov nas plasma uas tsim nyog tuaj yeem pab txuas tus qauv lom neeg nrog rau qhov kev ntsuas kom muaj nuj nqis ntau dua.

Xaus

NOD SCID nas plasma yog ib qho tseem ceeb lom zem matrix rau cov chaw kuaj mob koom nrog hauv kev tshawb fawb preclinical, bioanalysis, thiab kev tshawb fawb pharmacokinetic. Nws lub ntsiab kom zoo dua yog nyob rau hauv nws cov qauv tsim nyog: nws muab ib tug dawb paug matrix uas phim lub lom keeb kwm yav dhau ntawm NOD SCID system thiab txhawb ntau txhim khu kev qha analytical workflows.

Rau cov kws tshawb fawb ua haujlwm nrog cov qauv tshuaj tiv thaiv kab mob, xenograft systems, lossis kev tshawb fawb txog kev txhais lus ua ntej, NOD SCID nas plasma tuaj yeem yog qhov kev xaiv zoo thiab muaj txiaj ntsig zoo rau txoj kev txhim kho, calibration, thiab cov qauv tshuaj xyuas.