Unsatisfactory test results? The new version of the in-vitro micronucleus test kit is here to help!

PART 01. In-Vitro Micronucleus Test

Micronuclei are whole chromatids or acentric fragments or ring chromosomes which remain in the cytoplasm when chromosomes regularly enter daughter cells to form nuclei after mitosis. Because these fragments or chromosomes cannot enter the main nucleus at telophase, when the daughter cells enter the next interphase, they condense into small nuclei outside the main nucleus and form micronuclei. With the elucidation of formation mechanism, the significance of micronuclei, the continuous improvement of detection method and perfection of experimental techniques, micronucleus test became one of the main methods for screening mutations. It is widely used for predicting carcinogenic risk, detecting aneuploidy inducers and other genetic hazards.

The in-vitro micronucleus test is a genotoxicity test method for detecting the production of micronuclei in mammalian cells after treated with a test substance. It is ideal for checking chromosome breakage and aneuploidy induced in mitotic cells during/after exposure to the test substance. Compared to the in-vivo test, the in-vitro micronucleus test is simpler, faster, and cheaper. In addition, it is not affected by factors such as individual differences between test animals. Since in-vitro micronucleus test can detect micronucleus reactions induced by certain chemicals at low concentrations with high sensitivity. Therefore, it has been widely used in the field of genetic toxicology.


PART 02. In vitro micronucleus test kit

The IPHASE in-vitro micronucleus test kit uses Chinese Hamster Lung (CHL) cells as test system. The CHL cells are exposed to the test substance with/without metabolic activation system and treated with actin polymerization inhibitor cytochalasin B. The cells are allowed to reach their ideal frequency of binucleated cells, and then harvested, prepared, and stained; then the micronucleus rate of cells which completed one mitosis (binucleated cells) is analyzed under microscope to evaluate the mutagenic potential of the test substance. IPHASE kit provides all essential reagents and cells for in-vitro micronucleus testing.  Since all kits have passed strict quality testing, the produced experimental results are accurate, reliable and reproducible.  

--Product Advantages --

1. Convenience

Saves the time for induced S9 and reagent preparation,. Kit can be used directly, greatly shortening the experimental cycle.

2. Accuracy

Each component of the reagent kit has been subjected to strict quality testing, so the experimental results using the kit are accurate, reliable and highly reproducible.

3. Stability

The kit is stable and easy to transport and store.

4. Versatility

Wide range of applications, can be used for genetic toxicology testing of food, chemicals, pesticides, disinfectants, food additives, cosmetics, and many others.

Comparison of new and old kits

IPHASE follows the national standards and guidelines for in-vitro micronucleus tests in various fields as its technical guidance. Moreover, IPHASE takes customer feedback as its optimization goal to adjust the composition and methodology of the product and has successfully upgraded the in-vitro micronucleus test kits to meet more customer needs even after continuous verification.

1. Product Composition

Giemsa staining reagents and reagents required for the preparation process have been included to make it more convenient and efficient.

2. Methodology

The kit protocol is more detailed, with the inclusion of test precautions and other contents to avoid common mistakes.

PART 03. Related Products