Karohano ea Lisele tsa CD19 B ho tsoa ho PBMC Lipatlisisong tsa Botho ba Immunology

Mantsoe a sehlooho: Lisele tsa CD19 B, CD19 ea motho, B cell karohano, khetho e nepahetseng, khetho e mpe, ho hlopha lifaha tsa makenete, ho itšehla thajana ho MACS, ho itšehla thajana ha B cell, PBMC ho itšehla thajana, karohano ea lisele tsa 'mele, karohano ea lisele tse hoammeng, kit ea PBMC e leqhoa, khetho e ntle ea CD19, khetho e mpe ea CD19, li-lymphocyte tsa B tsa motho, kit ea ho hlopha lisele tsa B, antibody, khetho ea lisele tsa 'mele. karohano ea immunomagnetic

IPHASE Lihlahisoa

Lisele tsa CD19 B ke karolo e ka sehloohong ea lymphocyte tsamaisong ea 'mele ea motho ea ho itšireletsa mafung' me li sebelisoa haholo lipatlisisong tsa motheo le lithutong tsa phetolelo. Lits'ebetsong tse ngata tsa liteko, lisele tsa CD19 B li tsoa ho PBMC, e leng se etsang hore PBMC e be eona ntho e ka sehloohong e qalang bakeng sa ho ntlafatsa lisele tsa B. Lisele tsa CD19 B li emela karoloana e batlang e le nyane ea PBMC, empa li bohlokoa haholo bakeng sa tlhahiso ea masole a 'mele, taolo ea 'mele ea ho itšireletsa mafung, le mohlala oa mafu. Ka lebaka lena, lisele tsa CD19 B hangata li qheleloa ka thoko ho sebelisoa theknoloji ea immunomagnetic ho netefatsa bohloeki bo phahameng le bots'epehi ba ts'ebetso.

PBMC e le Mohloli oa Pele oa Lisele tsa CD19 B

PBMC e na le palo e fapaneng ea lisele tsa 'mele oa ho itšireletsa mafung, ho kenyelletsa le lisele tsa T, lisele tsa NK, li-monocyte, le lisele tsa CD19 B. Ka har'a motsoako ona, lisele tsa CD19 B li atisa ho ba teng ka lebelo le tlaase, e leng se etsang hore tlhahlobo e tobileng e be thata ntle le ho ntlafatsa pele. Ka hona, PBMC e sebetsa e le mohloli o tloaelehileng oa ho ikarola ha lisele tsa CD19 B. Lits'ebetsong tse ngata tsa laboratori, lisele tsa CD19 B li qala ho ntlafatsoa ho tloha PBMC pele li sebelisoa lits'ebetsong tse tlase tse kang flow cytometry, tatellano ea RNA, kapa liteko tse sebetsang.

Kamano pakeng tsa PBMC le CD19 B lisele ke ea bohlokoa liphuputsong tsa immunology. PBMC e fana ka moelelo oa cellular, ha lisele tsa CD19 B li emela palo ea batho ba ntšitsoeng tsamaisong ena. Karohano e nepahetseng ea lisele tsa CD19 B ho tloha PBMC e bohlokoa ka hona bakeng sa ho hlahisa lintlha tse tšepahalang tsa liteko.

CD19 B Cell Magnetic Bead Isolation Strategy

Ho itšehla thajana ha CD19 B ho tloaelehile ho sebelisoa ho sebelisoa mekhoa ea karohano e thehiloeng ho magnetic bead. Lisistimi tsena li itšetlehile ka sesole sa 'mele-lifaha tse kopaneng tse tlamellang ka ho khetheha liseleng tsa CD19 B ka har'a PBMC. Ha li se li ngotsoe, lisele tsa CD19 B li ka aroloa ho sebelisoa matla a khoheli, e leng se lumellang ho rua kapele ka litlhoko tse fokolang tsa lisebelisoa.

Tabeng ena, CD19 B cell isolation e ka etsoa ka khetho e nepahetseng kapa khetho e fosahetseng. Khetho e ntle e tšoara lisele tsa CD19 B ka kotloloho ho tsoa PBMC e sebelisa CD19-e shebileng lifaha tsa makenete. Mokhoa ona o hlahisa lisele tsa CD19 B tse matlafalitsoeng haholo tse loketseng lits'ebetso tse ngata tse tlase. Ka lehlakoreng le leng, khetho e mpe e tlosa lisele tse non-B ho PBMC, e siea lisele tsa CD19 B tse sa ameheng naheng ea habo tsona. Mekhoa ena ka bobeli e sebelisoa haholo, ho itšetlehile ka hore na liteko tse tlaase li hloka botšepehi ba receptor kapa bohloeki bo phahameng.

Khetho e Ntle le Khetho e Fosahetseng ea Lisele tsa CD19 B

Khetho e nepahetseng ea lisele tsa CD19 B e sebetsa hantle haholo bakeng sa ho fumana palo e hloekileng ea lisele tsa B. Ka mokhoa ona, lisele tsa CD19 B li ngotsoe ka ho toba le ho bolokoa sebakeng sa makenete, e leng se nolofalletsang ho rua ka potlako ho tsoa ho PBMC. Mokhoa ona o bohlokoa haholo ha ho hlokahala bohloeki bo phahameng, joalo ka litekong tsa tšebetso ea lisele tsa CD19 B kapa profiling ea transcriptomic.

Ka lehlakoreng le leng, khetho e mpe ea lisele tsa CD19 B, e etselitsoe ho boloka lisele tsa CD19 B ntle le ho tlama li-anti-antibody. Ka mokhoa ona, mefuta e meng ea lisele tsa 'mele ea ho itšireletsa mafung e fokotsehile ho tloha PBMC, ho kenyelletsa lisele tsa T, lisele tsa NK, le monocyte, ha lisele tsa CD19 B li ntse li sa fetohe. Mokhoa ona o ratoa ha ho ithutoa ts'ebetso ea lisele tsa CD19 B, litsela tsa lipontšo, kapa karabelo ea antigen, kaha e fokotsa ho kenella ha li-receptor tsa maiketsetso.

Ka bobeli khetho e ntle ea lisele tsa CD19 B le khetho e mpe ea lisele tsa CD19 B li amoheloa haholo liphuputsong tsa boits'ireletso ba motho, 'me mekhoa eo ka bobeli e qala ka PBMC joalo ka eona e qalang.

Karohano ea Immunomagnetic le Lisebelisoa tsa Teko

Karohano ea Immunomagnetic ke theknoloji ea mantlha ea ho itšehla thajana ea CD19 B. E fana ka mokhoa o potlakileng le o ka senyehang oa ho matlafatsa CD19 B lisele ho tloha PBMC ha o ntse o boloka ts'ebetso e phahameng. Tšebeliso ea li-antibody-lifaha tsa makenete tse koahetsoeng li etsa hore ho tlamahane ka mokhoa o ikhethileng, ho lateloe ke karohano e hloahloa e sebelisang likholomo tsa makenete kapa liraka.

Ka mor'a ho itšehla thajana, lisele tsa CD19 B li ka sebelisoa mefuteng e fapaneng ea lits'ebetso tse tlase, ho kenyelletsa le phenotyping ea 'mele ea ho itšireletsa mafung, tlhahlobo ea secretion ea cytokine, lithuto tsa B cell receptor, le ho sibolloa ha antibody. Boleng ba ho itšehla thajana ha lisele tsa CD19 B bo ama ka kotloloho ho ikatisa ha liteko, ho etsa hore ntlafatso ea ts'ebetso ea PBMC le liprothokholo tsa karohano e be tsa bohlokoa bakeng sa katleho ea lipatlisiso.

Ntlafatso ea Phallo ea Mosebetsi ho tloha ho PBMC ho ea ho CD19 B Cells

Ts'ebetsong e tloaelehileng ea laboratori, PBMC e qala ka ho qheleloa ka thoko ho lisampole tsa mali a motho ebe e sebelisoa e le tlatsetso bakeng sa ntlafatso ea lisele tsa CD19 B. Bokhoni ba ho arola lisele tsa CD19 B bo ipapisitse le lintlha tse joalo ka boleng ba PBMC, katleho ea ho tlama lifaha, le leano la khetho (khetho e ntle kapa e mpe). Litokisetso tsa PBMC tsa boleng bo holimo li lebisa ho boleng bo ntlafetseng ba CD19 B cell le bohloeki.

Ka kakaretso, ho itšehla thajana ha CD19 B cell ho tsoa ho PBMC ke mokhoa oa motheo oa ho itšireletsa mafung. Ebang ba sebelisa khetho e ntle ea lisele tsa CD19 B kapa khetho e fosahetseng ea lisele tsa CD19 B, bafuputsi ba itšetlehile ka PBMC e le qaleho ea bohlokoa bakeng sa ho hlahisa lisele tse sebetsang tsa CD19 B tse loketseng mefuta e mengata ea liteko.

Q1: Kamano ke efe lipakeng tsa CD19 B le PBMC?

Lisele tsa CD19 B ke karoloana ea batho ba PBMC. PBMC e na le mefuta e mengata ea lisele tsa 'mele tsa ho itšireletsa mafung, ho kenyelletsa lisele tsa T, NK lisele, monocyte, le CD19 B lisele. Lisele tsa CD19 B li emela karoloana e batlang e le nyane ea PBMC 'me hangata e ntlafatsoa ho tsoa ho PBMC bakeng sa liteko tse tlase.

Q2: CD19 B cell isolation e sebelisetsoa eng?

CD19 B cell isolation e sebelisoa ho fumana lisele tsa B tse hloekisitsoeng haholo bakeng sa lipatlisiso tsa immunology. Lisele tsa CD19 B hangata li qheleloa ka thoko ho PBMC bakeng sa lits'ebetso tse kang flow cytometry, tatellano ea RNA, ho sibolloa ha masole a 'mele, le liteko tsa tšebetso ea 'mele ea ho itšireletsa mafung.

Q3: Phapano ke efe lipakeng tsa khetho e ntle le e mpe bakeng sa lisele tsa CD19 B?

Khetho e ntle ea lisele tsa CD19 B e tšoara lisele tsa CD19 B ka ho toba ka ho sebelisa li-antibody- lifaha tsa makenete tse koahetsoeng, tse hlahisang bohloeki bo phahameng. Khetho e mpe ea lisele tsa CD19 B e tlosa li-non-B lisele ho PBMC, e siea lisele tsa CD19 B tse sa ameheng sebakeng se seng sa tlhaho. Mekhoa ena ka bobeli e sebelisoa haholo ho latela litlhoko tsa liteko.

Q4: Hobaneng ha PBMC e sebelisoa hangata bakeng sa ho itšehla thajana ha CD19 B?

PBMC ke sesebediswa se tlwaelehileng sa ho qala hobane se fumanwa ha bonolo mading a peripheral mme e na le disele tsa mmele tse kgolo tsa mononuclear, ho kenyeletswa le CD19 B cells. Ho sebelisa PBMC ho u fa monyetla oa ho ntlafatsa lisele tsa CD19 B ka lifaha tsa makenete -

Q5: Ke lisebelisoa life tse sebelisang lisele tsa CD19 B tse arohaneng le PBMC?

Lisele tsa CD19 B tse arohaneng le PBMC li sebelisoa haholo lipatlisisong tsa immunology le biomedical, ho kenyeletsoa tlhahlobo ea B cell receptor, lithuto tsa secretion ea cytokine, profiling ea 'mele ea ho itšireletsa mafung, le nts'etsopele ea meriana ea phekolo. Boleng ba ho itšehla thajana ha CD19 B bo ama ka kotloloho ho ts'epahala ha liteko tse tlase.