Lub hauv paus ntsiab lus ntawm lub Comet assay
Lub Comet Ansay, tseem hu ua ib qho - Cell gel electrophoresis, yog cov txheej txheem rau kev txiav txim siab GenotaToxicity los ntawm kev tshawb nrhiav DNA Strand kev puas tsuaj. Nws tuaj yeem pom zoo thiab muaj nuj nqis soj ntsuam cov ntaub ntawv DNA ib zaug thiab ob chav Strand Nick puas tsuaj hauv cov hlwb. When various endogenous and exogenous DNA damage factors induce cellular DNA strand breaks, its superhelical structure is damaged, and under the action of cell lysate, the cell membrane, the nuclear membrane and other membrane structures are damaged, and intracellular proteins, RNA, and other components diffuse into the cell lysate, whereas the nuclear DNA can only be retained in place due to its too large txheeb ze cov molecular loj.Nyob rau hauv nruab nrab kev mob, DNA tawg tuaj yeem nkag mus rau hauv gel thiab tsiv teb tsaws, dna yog de ntawm alkalized thiab dna dna tawg tawm thiab tawg tawm. Vim hais tias ntawm cov neeg txheeb ze me me ntawm cov dannular thiab cov alkaline dna mus rau hauv paus me me los mus rau hauv lub ntsej muag Nuclear tawm mus rau hauv cov duab hluav taws xob mus rau hauv cov duab hluav taws xob rau hauv cov duab DNA nyob twj ywm. Yog li ntawd, nws tuaj yeem yog cov lus qhia ntxiv rau hauv nruab nrab Comet xeem (ph = 8.4) thiab alkaline comet xeem (PH> 13) raws li cov cim sib txawv ntawm electrophoresis tsis. Tus qub yog siv los tshawb xyuas DNA ob zaug - strand breakage puas tsuaj, thaum lub sij hawm tau siv los kuaj me me ntawm ib qho - strand thiab ob zaug - strand breakage puas.
Feem ntau, qhov hnyav dua DNA kev puas tsuaj ntau, ntau qhov ntau dua nws ua, qhov me dua, thiab ntev dua kev tsiv teb chaws. Yog li ntawd, qib degree ntawm DNA kev puas tsuaj hauv ib leeg cell tuaj yeem txiav txim siab ntsuas ntawm DNA, yog li txiav txim siab qhov kev sib raug zoo ntawm kev ua yeeb yam ntawm cov ncauj lus thiab DNA puas tsuaj.
Cov khoom IIhase
Kev yooj yim: Cov reagents thiab consumables yuav tsum rau tib -Qhov tseeb: Txhua feem ntawm cov khoom siv tau raug kuaj nruj ua tau zoo, thiab cov txiaj ntsig kev sim yog qhov tseeb, txhim khu kev qha thiab muaj txiaj ntsig zoo.
Stability: Cov Khoom Siv yog ruaj khov thiab yooj yim thauj khoom thiab tshwj tseg.
Kev thov dav dav: Nws muaj ntau cov ntawv thov thiab tuaj yeem siv rau kev sim caj ces, tshuaj tua kab, tshuaj tua kab, khoom noj, cov tshuaj pleev ib ce, cov khoom siv tshuaj pleev ib ce, cov khoom siv tshuaj pleev ib ce, cov khoom siv tshuaj pleev ib ce thiab lwm yam.
Iphase tau mob siab rau hauv kev tshawb fawb kev tshawb fawb txog ntau xyoo, thiab tau kos duab rau kev tshawb fawb thiab muaj kev tshawb fawb zoo tshaj plaws. Khov Iphas muab cov cuab yeej muaj tseeb thiab muaj txiaj ntsig zoo los ua kom nrawm dua yug cov tshuaj tshiab; Nws tseem yog lub hnub qub ci ntsa iab nyob rau hauv qhov chaw tshawb nrhiav hauv lub neej, cov tswv yim tsis tu ncua nrog cov khoom tshiab, ua txhua yam dhia ntawm lub hauv ntej ntawm kev tshawb fawb. Hauv kev tshawb fawb txog kev paub txog Genota
Lub Sijhawm Post: 2024 - 07 - 26 15:08