Sulfotranferase (sul) bakeng sa lipatlisiso tsa DDI, botsitso ba metabolic, le thibelo ea enzyme

Mantsoe a Bohlokoa: Lits'ebeletso - Lits'ebeletso tsa lithethefatsi (DDI), sulfransferase, sulzyme, sulzyme, Hulzyme, Anzzyme

1 iphase e hlahisa litholoana

2 Khopiso ea Enzymatic nts'etsomong ea lithethefatsi

Nts'etsopele ea lithethefatsi, thuto ea metabolic phenotypes le thibelo ea enzyme ea metabolic e kang cyp450, ugg. E joalo. Metabolic phenotype e sebelisa mekhoa ea vitro e kopaneng le litsebi tsa metabolic, 'me ke li-mitaboli tsa tsona tsa koint Patlisiso ea Enzyme e bua ka litlhaselo tsa lithethefatsi kapa metsoako e metle ea metabolic (joalo ka li-melao-motheo tse fetohang / tse sa feleng ho bolela esale peleSethethefatsi - Tšebelisano ea lithethefatsi (DDI)Kotsi. Lithuto tsena li fana ka lebaka la ho matlafatsa moralo oa lithethefatsi, ho hlahloba polokeho (joalo ka ho lekola metriboolites), le ho tataisa meriana e hlakileng. Phephetso ea mantlha e fetoloa ho vitro ho ea ho data ea vivo ho ea ho data ea vivo le ho lemoha ho lemoha ho lemoha ho lemoha metabotolites e tlase e tlase. Nakong e tlang, mefuta e tsoetseng pele e kang Metsoako e ka sebelisoa ho matlafatsa ho tšepahala ha lipatlisiso.

3 sulfotransferase (sul)

Sulfotransferase (sul)ke mofuta oa ho fetisoa ha ho fetisoa ha lihlopha tse sulfate 'me ho ameha metsoako le metsoako ea hae (joalo ka litlhare tsa tikoloho). Li-sulftransferansferals li fumaneha haholo ka har'a lisebelisoa tsa cytoplasm, tse tloaelehileng ka har'a li-elecle tse nyane (tse kang lithethefatsi, li-protheines, li-protheines, glycosaminoglycans). Li-sulfotrolsferasferas li khetholloe ho ba le li-subtypes tse ngata, haholo ho kenyelletsa sul1, Surt2 le sul4. Ho hlokahala hore motho e se ke a etella pele ho senya metabolism ea lithethefatsi, kankere, mathata a endocrerine le mathata a se ke a ba kotsing.

4 sulfation / sulfination metabolism

Sulfination Metabolism (e tsejoang ka hore ke metabolism) e phethang litheko tsa lithethefatsi le tšebeliso ea lithethefatsi tse ncha tsa lithethefatsi le tšebeliso e teng ea bongaka. Sulfottransferase ea motho (e tsejoang e le sulfatase) e na le likarolo tse ngata haholo 'meleng, haholo-holo li-organs tse kang sebete, mala a manyane. Lithaba tse tloaelehileng tsa motho li kenyelletsaSulza1, Sulza3, Sult1b1, Sul1c2, leSulthic4 (Tafole 1). Bakeng sa lintlha tse ngata, sulfonfensfensfensfenfensfensfenfensfensfensfensfenshian metabolism hangata li bonts'a litšobotsi tse tloaelehileng tsa thibelo; Mehato e tlase ea substrate hangata e ama polelo ea enzyme.

Lethathamo la 1 kabo le ts'ebetso ea sulter e arolelanoang hantle 'meleng oa motho

Likopo tse 5 tsa senotlolo tsa sulf nts'etsopele ea lithethefatsi

5.1 Tlhahlobo ea vitro ea DDI e ipapisitse le metabolism

The Vitro Pusitso ea Sehlekehleke sa suul e etsoa haholo ka lithibelo tse ikhethileng (joalo ka litsamaiso tsa enzplas tsa li-sytoppe kapa tsa selemmminant ka li-swestpe tse khethehileng tsa sultypes. Hangata moralo oa liteko o kenyelletsa:

Tlhahlobo ea Metabolic Phenotype: Efihlela sekhahla sa metabolited moloko oa metaboliteteration ka lc - ms mme o bala sekhahla sa ho thibela (boleng ba IC50 / kil).

Bohlale ba kotsing ea tlhaho: Haeba inhibitor a fokotsa tlhahiso ea metabono> 50%), e fana ka maikutlo a hore ho netefatsoa ho feto-fetoha.

5.2 SURT Metabolic Thutong ea botsitso

Nts'etsopele ea lithethefatsi, thuto ea botsitso ba metabolic e etsoa ka sekhahla sa phapanyetsano le ho beha ha lipalesa tsa metabolation, 'me u tataise hore u tsebe ho fana ka moea oa metaboli.

5.3 Patlisiso ea Metabolistra

Mokhoa oa ho etsa lipatlisiso (Metabolic Patway Pathway) o ka supa moralo oa li-enzyme tsa k'homphieutha, ebe o li kenya ka lithibelo tsa Gecko skminases le ho bala litšebeletso tsa bona tsa Generanti.

Molao-motheo oa sehlooho le Tsela ea Bohlale ba Teko ea li-cult ke ho hlahloba hore na metabolism ea magical e thibela ho se sebetse hantle

Litefiso tsa enzyme tsa Enzyme

SUR e kenella metabolism ea lintho tse ngata tse emisitsoeng tsa matla le lithethefatsi. Haeba ho ntse ho etsoa lithethefatsi ho thibela mosebetsi oa setulong, ho ka ba le litaba tsa ts'ireletso ntle le ho arolelana lithethefatsi. Molao-motheo oa sehlooho le Tsela ea Bohlale ba Stolt Enzyme Of Assayme ke ho sebelisa sirapo ea enwane ea Sluwer ho thibela meriana le lipapatso tsa lipatlisiso tse kang p - nitrophenol.

Liphello

Sulfotransferase (sul) e bapala karolo ea bohlokoa ea metabolism, lithethefatsi - Ho sebelisana ha lithethefatsi (DDI) le tlhahlobo ea ts'ireletso. Ho tsamaisana le metabolism ho ama ho hloekisoa hoa lithethefatsi, ts'ebetso, kapa chefo ea tikoloho ea li-rilical), ha lithupa tsa tikoloho li ka beha kotsi ea lefu la Clical DDI. Ka ho kopanya mefuta ea vitrolm (liver cytoplasm, enzzle e nchafalitsoeng Nakong e tlang, mefuta e tsoetseng pele e le bakhaolo e tla ntlafatsa boleng ba thuto ea sulul, e fana ka botsitso bo nepahetseng ba metabolic le polokeho ea bongaka.

Resort

Li, y., Lindsay, J., Wang, L. L., & Zhou, S. (2008). Sebopeho, tšebetso le polyrmorfismphism ea batho e sulfransfensferansferasferasesferases. Metabolism ea Sechaba, 9(2), 99 - 105.