Ames bvunzo yeN - Nitrosamines: Kuongorora Mutegenicity

Nhanganyaya kuAmes bvunzo

A maals test, anozivikanwawo sehutachiona hwekutsvaira musi muedzo unoshandiswa zvakanyanya neyekuzvarwa newakashandiswa newaya muMutagetic kugona kwemakemikari, kusanganisira n - nitrosamini. Yakagadzirwa naDr. Bruce ames muma1970s, uyu muedzo unoratidza chaiwo hutachiona hwehutachiona hwehutachiona hweTyphumurium iyo inotakura kuzvitunha muHiniidine synthesis. Muedzo unosarudza kana chinhu chinogona kukonzera kuzvitaura muhutachiona, kupa chiratidzo chevanogona carcinogenityity muvanhu.

Kukodzera kweAmes bvunzo yeN - Nitrosamines

N - Nitrosamini anozivikanwa nekwavo uye carcinogenic zvivakwa, sezvo zvavanogona kuwana DNA kukuvara kuburikidza nekushushikana uye kusagadzikana uye oxidative kushushikana. Miedzo yeAmes inonyanya kubatsira mukuona kwavo Muttagenic madhara Active iyi inowanzoitika mune chiropa kuburikidza cytochrome P450 enzymes. Kuti udzorere izvi metabolic kushandurwa mu vitro, bvunzo dzinowanzoitwa neye metabolic activation uchishandisa SME, iyo inotungamirwa metentian metabolism uye inosimudzira kuongororwa kweMutagetic basa.

Maitiro eAmberi

Maitiro akajairwa eAmes bvunzo inosanganisira matanho anotevera:

1. 1. Kugadzirira kweTest Starins: Salmonella Typimburium Starins nePre - Vairipo Muviri muHindidine Synthesis genins inoshandiswa. Aya matanho haagoni kukura pasina wekunze henteidine sosi kunze kwekunge adzoreredza mutation kudzoreredza basa.
2. 2. Kuratidzwa kuN - Nitrosamines: Iyo tsika yehutachiona yakasanganiswa neye bvunzo compound (n - nitrosamine) pane diki agar ndiro ine huwandu hushoma hwe histidine.
3. 3. Metabolic Activation
4. 4
5. 5. Colony kuverenga uye kuongororwa: iyo nhamba yeyedzoreredzwa colonies (mabhakitiriya akawanazve kugona kuburitsa histine) anoverengerwa uye achienzaniswa nemirazvo yekudzora.
6. Kududzira kwemhedzisiro

• Echokwadi AMES Test: Kuwedzera kwakakosha mumakoreti anozoenzaniswa nekudzora kunoenderana neyakaomeswa madhiri ekuzvishandura, achireva Mutangelic uye anogona carcinogenic zvivakwa.
• MaTestamedza Vakaedza Bvunzo: Kana pasina kuwedzera kwakakosha kucherechedzwa, iyo compound ingangove isiri - mu mumagenic pasi pemamiriro ekuedzwa.
• Doose - Kuparidzira Ukama: Madhiri epamusoro anotungamira kuwedzerwa mutengo wekutora anosimbisa humbowo hwe muutagenicity.

Mhedziso

Iyo maABES bvunzo ndeyekukurumidza uye mutengo - nzira inoshanda yekuongorora Mutegenicity yeN - Nitrosamines. Kupiwa kusonganirana kwavo nekenza, kuzivisa zvivakwa zvavo mumagegenic kuburikidza neiyi assay inokosha yekudzora kudzora uye kuongororwa njodzi. Iyi bvunzo inoramba iri kongiri mune ineologicologication yekuongorora uye kemikari yekuchengetedza kuongororwa.

Keywords: N - Nitrosamines, NDSris, OEECD Test, Hamster Levers S9, Cytochrome P450 enzation, Mutest bvunzo