Ames Gwaji ga N - Nitrosames: Kimantawa mutagicaity

Gabatarwa ga gwajin ames

Ames na gwaji, wanda kuma aka sani da aka sani da aka sani da kayan juyi na juji ana amfani da shi sosai don maganin asarnay wanda ke kimanta damar sinadarin sunadarai, gami da N - Nitrosamines. Dr. Bruce Ames a shekarun 1970, wannan gwajin yana amfani da takamaiman jigon ƙwayoyin cuta wanda ke ɗaukar maye. Gwajin yana yanke shawara ko abu na iya haifar da maye gurbi a cikin DNA DNA, yana ba da alama game da carciniyanci a cikin mutane.

Amincewa da Ames Gane Ga N - Nitrosamines

N - Nitrosamines sanannu ne da kaddarorinsu na genotoxic da carcinogenic, kamar yadda zasu iya haifar da lalacewar DNA ta hanyar rashin ƙarfi da oxdational danniya. Hukuncin ames yana da amfani musamman yana da amfani wajen gano tasirinsu na mutawic, kamar yadda mutane da yawa n - da yawa juyawa na lantarki mai saurin yin hulɗa da DNA. Wannan kunnawa yawanci yana faruwa ne a hanta ta hanyar Cynitchrrome P450 enzymes. Don kwaikwayon wannan jujjuyawar metabolic a cikin vitro, ana yin gwajin tare da kuma ba tare da hukumar s9, wanda metabolic metabolic da kuma inganta gano ayyukan mutalenic ba.

Hanyar Gwajin Ames

Standarda ta tabbatar da gwajin ames ta ƙunshi matakan masu zuwa:

1. 1. Shiri na gwaji na gwaji: salmonella Typhimurium tare da pre - An yi amfani da maye gurbi a cikin tsarin ilimin halittar gaske. Waɗannan ƙwayoyin ba za su iya girma ba tare da tushen tarihi na waje ba har sai wani juzu'i na juzu'i.
2. 2. Fitar da N - Nitrosames: al'adun ƙwayoyin cuta ana hade shi da filin gwajin (n - nitrosamine) a kan karamin farantin tarihin tarihi.
3. 3. Kunna fetabolic (s9 ƙari Bugu da kari): Wasu samfuran gwaji sun haɗa da juzu'i na S9, enzymatic cirewa daga bashin.
4. 4. Yin shiryawa da girma: An sanya faranti a cikin 37 ° C na 48, suna ba da damar yin girma idan maye gurbi yana dawo da tsarin tarihin.
5. 5. Saukake da ƙasa da bincike: yawan ƙasan mulkin mallaka (ƙwayoyin cuta waɗanda ke dawowa da ikon samar da tarihin tarihi) an kwatanta su da ikon sarrafa faranti.
6. Fassarar sakamako

• Tabbatacce ne na Gwaji: Babban karuwa cikin mazauna unguwar mulkin mallaka idan aka kwatanta da mahaɗan yana nuna maye gurbi, yana nuna abubuwan da suka dace da kayan masarufi.
• Ba shi da matsala game da gwajin: Idan babu karuwar ƙaruwa mai mahimmanci, fili mai yiwuwa ba - mutagenic karkashin yanayin gwajin.
• SANAR - Dangantaka ta amsa: Desesland mafi girma da ke haifar da ƙara girman darajar maye gurbi yana ƙarfafa shaidar mutonicity.

Ƙarshe

Ames na gwaji ne mai sauri da farashi mai inganci don kimanta mutagility na N - Nitrosamines. Ganin ƙungiyarsu, da cutar kansa, suna gano kaddarorinsu na mutageic ta wannan muhimmiyar mahimmanci ce ga sarrafawa da ƙididdigar haɗari. Wannan gwajin ya kasance babban tushe a allo mai guba da kimantawa na aminci na aminci.

Keywords: N - NICrosames, NdSris, OECD 471, Ingantaccen Harkar Hams S9, Entoler S9, enzymes