Mayeso a ames a N - Nitrosamines: Kuyesa Mutagenicity

Mawu oyambira mayeso a Ame

Mayeso a ameni, omwe amatchedwanso mayeso osintha madongosolo amagwiritsidwa ntchito kwambiri chifukwa chovuta kwambiri chomwe chimawunika poyerekeza ndi mankhwala a Mutagenic, kuphatikiza n - Nitrosuamine. Wopangidwa ndi Dr. Bruce ames mu 1970s, mayeso awa amagwiritsa ntchito zigawo zina za bacterium Typhimu Unimurium yomwe imatengera masinthidwe a histridine. Kuyeserera kumatsimikizira ngati chinthu chomwe chingapangitse masinthidwe mu bakiteriya, ndikupereka chisonyezo cha kuchepa kwa mpweya womwe ungayambike mwa anthu.

Kufunika kwa mayesedwe a ames kwa n - Nitrosines

N - Nitrosamines amadziwika chifukwa cha mtundu wawo wa genotoxic awo ndi carcinogenic, popeza angapangitse kuwonongeka kwa DNA kudzera pa ma alkylation ndi opsinjika. Mayeso a Ames ndiofunika makamaka pakuwona zotsatira zawo za Mutagenic, monga n - Nitrosines amafunikira kuyambitsa ma enzymofic kuti apange apakati otha kulumikizana ndi DNA. Izi zimachitika mu chiwindi kudzera mu cytochrome p450 michere. Kuti mumvetsetse kutembenuka ka mankhwala mu vitro, mayeso nthawi zambiri kumachitika ndi kusakaniza kwa metabolic pogwiritsa ntchito ma sements, omwe amalimbikitsa penticles metabolism ndikuwonjezera mwayi wofufuza za Mutagenic.

Njira ya AMES

Njira yoyeserera ya ames imaphatikizapo zotsatirazi:

1. 1. Kukonzekera kwa Mayeso Oyeserera: Salmollayla Typhimuulium ndi Presphimuulium ndi Pres - Masinthidwe omwe ali mu histridine synthesis majini amagwiritsidwa ntchito. Zitseko izi sizingamere popanda gwero lakale lakale pokhapokha ngati nthawi yosinthira ikubwezeretsa ntchito.
2. 2. Kuwonetsedwa ku N - Nitrodamines: chikhalidwe cha mabakiterite amasakanikirana ndi mayeso (n - Nitrosine) pa mbale yocheperako yomwe ili ndi hirdidine wocheperako.
3. 3. Kuyambitsa metabolic (s9 kusakaniza kosinthana): Kuti muyankhe kutembenuka kagayidwe kamunthu m'thupi la munthu, zitsanzo zina zoyeserera zimaphatikizapo gawo la S9, enzymatic imachotsa microsomes.
4. 4. Kukula ndi Kukula: Mbaleyo amalizidwa pa 37 ° C kwa maola 48, kuloleza matomiya mabakiteriya kuti akule ngati masinthidwe omwe amabwezeretsa Rididisine kaphatikizidwe kakale.
5. 5. Kuwerengera Colony ndi kusanthula: kuchuluka kwa magulu obwera (mabakiteriya omwe adayambanso kupanga hirbidine) amawerengedwa ndikuyerekeza ndi mbale zowongolera.
6. Kutanthauzira kwa Zotsatira

• Kuyesa Kwabwino kwa ames: Kuwonjezeka kwakukulu kwa ma counties obwereza poyerekeza ndi kuwongolera komwe kumawonetsa mphamvu zophatikizira, kutanthauza mphamvu ya Mutagenic komanso yomwe ingakhalepo.
• Kuyesa kwa AMEES: Ngati palibe kuwonjezeka kwakukulu komwe kumaonedwa, kuwirikiza mwina si - Mutagenic pansi pa mayeso.
• Mlingo - Ubale Woyankha: Mlingo wokwera wotsogolera kuti uwonjezere masinthidwe osintha masinthidwe amalimbitsa umboni wa Kumisala.

Mapeto

Kuyesa kwa ames ndi kofulumira komanso mtengo - njira yoyeserera yowunikira Mutagenicity wa n - Nitrosines. Anapatsidwa mayanjano awo ndi khansa, kuzindikiritsa mphamvu zawo kudzera mu lingaliro ili ndikofunikira kuti alamulire ndi kuyesedwa pachiwopsezo. Kuyesa uku kumakhalanso mwalawapambano wowunikira ndi kuwunika kwa mankhwala.

Mawu osakira: N - nitrosamine, NDSris, OECD 471 Kuyesa kwa AMEES