Ames xeem rau n - Nitrosamines: Kev tshuaj xyuas mutagenicity

Taw qhia rau Ames xeem

Tsab ntawv qhia txog Ames, tseem hu ua cov kab mob thim rov qab yog qhov kev ntsuas txuas dav rau kev ntsuas cov tshuaj mutagenic, suav nrog n - nitrosamines. Tsim los ntawm Dr. Dr. Bruce Ames nyob rau xyoo 1970, qhov kev ntsuas no siv cov kab mob salmonella typhimurium uas muaj kev sib pauv hauv cov khoom siv hluav taws xob hauv histicesis. Qhov kev xeem txiav txim siab seb puas muaj peev xwm ua rau cov kev sib raug zoo hauv cov kab mob DNAs, muab kev qhia ntawm cov neeg muaj peev xwm carcinogenicity hauv tib neeg.

Qhov tseeb ntawm cov ames xeem rau n - nitrosamines

N - Nitrosamines paub txog lawv cov khoom lag luam genotoxic thiab carcinogenic cov zog, raws li lawv tuaj yeem ua tsis taus kev puas tsuaj DNA los ntawm kev ntxhov siab thiab oxidative. Qhov kev xeem Ames yog pab tau tshwj xeeb hauv kev tshawb xyuas lawv cov kev hloov pauv ntawm Enzymatic hloov dua siab tshiab los tsim cov khoom siv hluav taws xob siab los ntawm kev sib cuam tshuam nrog DNA. Qhov kev ua tau zoo no feem ntau tshwm sim hauv daim siab dhau ntawm Cytochrome P450 enzymes. Txhawm rau repicate no metabolic conversion hauv vitro, feem ntau yog feem ntau ua nrog thiab tsis muaj kev sib xyaw rau nas, cov mimics

Txheej txheem ntawm Ames xeem

Cov txheej txheem ntawm Ames xeem yuav cuam tshuam nrog cov kauj ruam hauv qab no:

1. 1. Kev npaj ntawm Kuaj: Salmonellurium hom nrog ua ntej - kev hloov pauv hauv horidine cov khoom siv hluav taws xob yog siv. Cov hom dej no tsis tuaj yeem loj hlob yam tsis muaj lub keeb kwm sab nraud sab nraud yog tias kev thim rov qab rov qab muaj nuj nqi.
2. 2. Raug rau NS - Nitrosamines tau sib xyaw nrog cov ntawv xeem (n - Nitrosamine) ntawm qhov chaw siab me me uas muaj cov nyiaj ntau ntawm histidine.
3. 3. Kev ua haujlwm metabolic ua kom (s9
4. 4. Txhim kho thiab kev loj hlob: Cov phiaj hlau yog tsim ntawm 38 teev, pub cov kab mob sib tsoo yog kev hloov pauv ntawm synthesis.
5. 5. Ib lub nroog suav thiab kev tsom xam: tus naj npawb ntawm revertant Colonies (cov kab mob uas tau rov qab muaj peev xwm tsim cov phiaj xwm los tsim cov phiaj.
6. Txhais ntawm cov txiaj ntsig

• Kev ntsuas Ames muaj txiaj ntsig zoo: ib qho kev nce qib tseem ceeb hauv kev tswj hwm pom cov compound tsim kev sib hloov, cuam tshuam mutagenic thiab muaj peev xwm carcinogenic cov khoom.
• Pom zoo Ames xeem: Yog tias tsis muaj kev nce qib tseem ceeb, qhov sib xyaw ua ke yog tsis yog - mutagenic nyob rau hauv cov kev ntsuas.
• Koob tshuaj - Kev sib raug zoo tshaj tawm: Ntau dua cov koob tshuaj ua rau muaj kev sib hloov zuj zus ntxiv rau cov pov thawj.

Tag

Qhov kev xeem Ames yog kev nrawm thiab tus nqi - Cov qauv siv tau zoo rau kev txheeb xyuas cov kev sib hloov ntawm n - nitrosamines. Muab lawv lub koom haum nrog mob qog noj ntshav, txheeb xyuas lawv cov khoom mutagenic los ntawm cov khoom dab tsi yog qhov tseem ceeb rau kev tswj hwm thiab kev tshuaj xyuas kev pheej hmoo. Qhov kev xeem no tseem yog lub hauv paus hauv lub hauv paus hauv kev tshuaj ntsuam xyuas kev nyab xeeb thiab tshuaj lom neeg kev nyab xeeb.

Cov ntsiab lus: N - Nitrosamines, NDSris, OECD 471, Hamster Lier Kev Ntsuas, Hamster Lier S9, Cytochrome P450 Enzymes, Hloov Mus Kuaj