Taw qhia rau MRNA Cov Tshuaj
Cov Neeg xa xov lna (MRNA) yog ib ntu ntawm cov protein uas yog cov protein, thiab hauv cytoplasm muaj peev xwm siv cov orgaded protein. MRA cov tshuaj muaj kev hloov kho raws li kev xaiv ntawm cov hom phiaj tsim qauv lossis nkag mus rau lub hom phiaj kev hloov tshuaj (E. Tus kws kho mob tuaj yeem muab txhais ua cov protein, thiab ib qho tshuaj uas siv cov protein uas kho tau los ntawm MRNA kev kho mob.
Daim duab 1. Cov qauv tshuaj ntawm MRNA cov yeeb tshuaj thiab cov txheej txheem uas lawv tau siv ua lawv txoj kev ua tau zoo
Cov tswv yim rau kev tshawb fawb ntawm cov tshuaj ntawm cov tshuaj MRAN
Hais txog cov tshuaj ua ntej lub chaw muag tshuaj ntawm MRNA cov tshuaj, nws tuaj yeem faib ua peb ntu kev tshawb tshuaj uas cov tshuaj tiv thaiv MRA thiab cov tshuaj zoo nkauj thiab cov khw muag khoom siv. Raws li cov tshuaj tiv thaiv - Cov lus qhia kev paub txog los ntawm FDA thiab NMPA, cov tshuaj tiv thaiv feem ntau tsis tas yuav tsum tau kawm cov tshuaj tiv thaiv tshwj xeeb yuav tsum tau kawm rau kev ua haujlwm biudish. Cov tshuaj tiv thaiv MRA tuaj yeem muaj cov tshuaj tiv thaiv tshwj xeeb, uas yuav tsum muaj cov kev tshawb fawb biffispious. Thiab cov tshuaj ntsiav tshuaj ntawm MRAA cov tshuaj kho mob tuaj yeem pab kom nkag siab txog qhov ntau ntawm cov tshuaj. Txog cov chaw tsim tshuaj paus tshiab, xws li cov ntaub ntawv sau qoob loo lossis lwm yam kev siv tshuaj thiab cov lus qhia kev nyab xeeb ntawm cov chaw tshuaj tiv thaiv kev nyab xeeb ".Hauv kev tshawb fawb vitro muaj teeb meem metabolic thiab cov metabolite ntawm cov tshuab ntau yam, thiab hauv Vivo txoj kev kawm muaj kev nqus, kev faib tawm, cov kev tshawb fawb thiab cov kev tshawb fawb ua yeeb yam hauv cov tsiaj ntawm Vivo Adme tus txheej txheem ntawm kev zam. Kev siv yeeb tshuaj - Cov tshuaj sib cuam tshuam ntawm cov khoom siv tshiab tau tshuaj xyuas nyob ntawm theem kev pheej hmoo.
IPhase cov khoom lag luam
Cov Neeg xa xov lna (MRNA) yog ib ntu ntawm cov protein uas yog cov protein, thiab hauv cytoplasm muaj peev xwm siv cov orgaded protein. MRA cov tshuaj muaj kev hloov kho raws li kev xaiv ntawm cov hom phiaj tsim qauv lossis nkag mus rau lub hom phiaj kev hloov tshuaj (E. Tus kws kho mob tuaj yeem muab txhais ua cov protein, thiab ib qho tshuaj uas siv cov protein uas kho tau los ntawm MRNA kev kho mob.
Daim duab 1. Cov qauv tshuaj ntawm MRNA cov yeeb tshuaj thiab cov txheej txheem uas lawv tau siv ua lawv txoj kev ua tau zoo
Cov tswv yim rau kev tshawb fawb ntawm cov tshuaj ntawm cov tshuaj MRAN
Hais txog cov tshuaj ua ntej lub chaw muag tshuaj ntawm MRNA cov tshuaj, nws tuaj yeem faib ua peb ntu kev tshawb tshuaj uas cov tshuaj tiv thaiv MRA thiab cov tshuaj zoo nkauj thiab cov khw muag khoom siv. Raws li cov tshuaj tiv thaiv - Cov lus qhia kev paub txog los ntawm FDA thiab NMPA, cov tshuaj tiv thaiv feem ntau tsis tas yuav tsum tau kawm cov tshuaj tiv thaiv tshwj xeeb yuav tsum tau kawm rau kev ua haujlwm biudish. Cov tshuaj tiv thaiv MRA tuaj yeem muaj cov tshuaj tiv thaiv tshwj xeeb, uas yuav tsum muaj cov kev tshawb fawb biffispious. Thiab cov tshuaj ntsiav tshuaj ntawm MRAA cov tshuaj kho mob tuaj yeem pab kom nkag siab txog qhov ntau ntawm cov tshuaj. Txog cov chaw tsim tshuaj paus tshiab, xws li cov ntaub ntawv sau qoob loo lossis lwm yam kev siv tshuaj thiab cov lus qhia kev nyab xeeb ntawm cov chaw tshuaj tiv thaiv kev nyab xeeb ".Hauv kev tshawb fawb vitro muaj teeb meem metabolic thiab cov metabolite ntawm cov tshuab ntau yam, thiab hauv Vivo txoj kev kawm muaj kev nqus, kev faib tawm, cov kev tshawb fawb thiab cov kev tshawb fawb ua yeeb yam hauv cov tsiaj ntawm Vivo Adme tus txheej txheem ntawm kev zam. Kev siv yeeb tshuaj - Cov tshuaj sib cuam tshuam ntawm cov khoom siv tshiab tau tshuaj xyuas nyob ntawm theem kev pheej hmoo.
IPhase cov khoom lag luam
| Pawg | Kev faib ua |
| Subcellular feem | Mob siab Lysosome |
| Acidified li siab lub siab homogenate | |
| Daim siab / hnyuv / raum / Lungs S9 | |
| Mob siab / plab / raum / LUNG microsomes | |
| Daim siab / plab hnyuv / lub plab zom mov / qog cytopeplasmic kua | |
| Thawj Hepatocytes | Raug tshem tawm Hepatocytes |
| Platable Hepatocytes | |
| Tshwj xeeb ntshav | Ntshav ruaj khov |
| Ntshav cov protein khi | |
| Tag nrho cov ntshav | Tib neeg / liab / canine / nas / nas / luav / npua dawb paug ntshav |
Lub Sijhawm Post: 2024 - 08 - 25 19:54:01