Mai ka wā e emi ana nā hana metabolizing e like me ka hanaʻana i ka manawa o Vitro IncHTO INTORT A ME KA WELIKINTER. Nā lāʻau lapaʻau[1]. ^. I lanau,hepatocyteS loaʻa hou nā enzymes e koiʻia no kaʻaelike, hoʻemiʻia, hoʻemi,ʻo hydrolysis a me nā mea hou e hoʻohālikelikeʻiaNā momi Kelepona, a i loko o nā'ōnaehana vitro, he nui nā mea hoʻohālike nui e nānāʻia e pili ana i kahepatocyte 'ōnaehana a me nā momic hou i loko o kaʻenehana vivo e hōʻoia i ka hoʻomauʻana i ka hana o nā hana enzyme i lokohepatocyteS, Aʻo ka hoʻomaikaʻiʻana o ka manawa hoʻomohala e hāʻawi ai i hiki ke kiʻi i ka loliʻana o ka metabolikaping lāʻaul[1,2].
I kaʻikeʻana i kēia,ʻo Iphise, he alakaʻi i nā mea hou aku no VITRO,ʻo ka mokuahiʻo Hepatomax e hoʻohana ana i ka Hoʻohui pilikinohepatocytes like me kahi hoʻohālike, a ua hoʻonohonohoʻia nā'ōnaehanaʻelua no ka hoʻoholoʻana o ka hana a me ka loaʻaʻana o ka waiwai o nā lāʻau lapaʻau i nā lāʻau lapaʻau. Hoʻonui ka metabolilite i ka waiwai pane i nā lā 10 a me ka hana a me ka hana a me ka metabolic o nāʻano likeʻole ma 7 mau lā e hōʻoia ai i nā'ōnaehana.
- (1)ʻO nā kaʻina hana i hoʻopaʻaʻia e nā kaʻina hana lapaʻau
Ipha make - hepatomax ™he CO -ʻO ka'ōnaehana moʻomeheu o ka brozen i hele i nā hepatocle mua a me nā cells stell. Ua hana houʻiaʻo Hepatocytes kanaka mua a hoʻokumuʻia me nā cell stromal i ka lāʻau lapaʻau. ʻO ka huina o 4, 72, 168 a me 240 mau hola ma hope o ka hoʻokele lāʻau, ua loaʻa ka waiwai o ka lāʻau lapaʻau no ka hoʻopiliʻana i ka lāʻau lapaʻau.
Iphie - hepatocon ™, ma kaʻaoʻao'ē aʻe, he'ōnaehana no ka moʻomeheu frofen adherent he nui o ka hepatocytes kanaka ma hepatocon ™ Medium. Ma hope o ka hoʻihoʻiʻana i ke kanaka muahepatocyteS, ua hanaʻia ka waiwai o ka lāʻau lapaʻau ma hope o 3 mau lā o ka moʻomeheu ma Hepatocoile hoʻokahi
ʻO ia hoʻi, e hōʻoia i ka pololei a me ka ikaika o nā hopena,ʻo Iphase i hana i kahi mana kikowaena, a me nā hua'ōlelo heʻekolu.Ipha make - hepatomax ™ aIphie - hepatocon ™ Hoʻopili nā'ōnaehana i ka pane ikaikaʻo PEAK ma kahi o ka lā 3 i keʻano 3 ma nā manawa he nui, a ua hōʻikeʻia lāua i kahiʻano ikaika ikaika loa.
- (2) Ke hilinaʻi nui nei ka metabolism i ka hepatocyteCyp450ʻO ka'ōnaehana enzyme, a hiki i kāna hana ke ana i ka hoʻomaʻamaʻa maikaʻi aʻe.
'ŌleloIpha make - hepatomax ™ aIphie - hepatocon ™ Loaʻa nā'ōnaehana maikaʻi.
Ua hoʻonohonohoʻo Iphise i nā'āpanaʻehā o ka mana o ke kikowaena (ʻaʻohe cell), hepatomax ™, hepatocon ™ a me nā cell crosrtal. Ke hoʻohana nei i ka pā.hepatocytes e like me kahi hoʻohālike, hoʻonui i nā waiwai o ka metaboliteCyp1a2, Cyp2b6aKālā3Ma4ua hōʻoiaʻia i ka lā 0, 3 a 7 ma hope ihohepatocyteʻO ka ho'ōla no 3 mau lā e hoʻohana ana i ka hanaʻana, broppion a me badazolam a me nā mea e like me ka mea i kauʻia. Hoʻonuiʻo Metabolite i nā waiwai i hōʻikeʻiaIpha make - hepatomax ™ aIphie - hepatocon ™ Hōʻike nā'ōnaehana i ka hana enzyme ikaika e ka lā 7, e hoʻolaha mau ana i nā lāʻau lapaʻau maʻamau maʻamau.
- (3) Ma kahi o ka hōʻoia o ka hana enzyme, he mea koʻikoʻi a metabolicty maikaʻi loa a me ka hanaʻole i ka hana o nāʻano vitro.
Ua hoʻonohonohoʻo IPase iʻekolu mau helu o Hepatomaxhepatocytes, a me nā hopena i hōʻike i nāIpha make - hepatomax ™ aIphie - hepatocon ™ Hōʻike nā'ōnaehana i nā hana i ka hana clearance metabolic i loko o ka waiwai lōʻihi loa o ka manawa waiwai.
I ka hōʻuluʻuluʻana,ʻo Iphise, he alakaʻi i loko o Vitro Bio - Regents, ua kūkuluʻiaIphane Hepatomax ™ aIahise HePATCONCON ™ i nā hiʻohiʻona Vitro e pili ana i ka Wall - i noi i ke kanaka mua, Monkey, Canine, Rat a me MousehepatocyteS a me nā cells stromal o nāʻano likeʻole, me ka nui o nā hana a me nā mea e hoʻonui ai i nā mea kūʻai aku. 'ŌleloIphane Hepatomax ™ aIahise HePATCONCON ™ I nā hana Vitro e hāʻawi i nā hana heʻe kīʻaha metabolic he mau hana likeʻole, nā hana'ē aʻe he nui no nā mea kūʻai aku.
Kākau:
[1] 阮婷婷, 鞠武建, 熊海伟, 熊海伟. 低清除率药物低清除率药物 · 代谢稳定性预测模型研究进展 [19,10 (20 - 160 - 160. 160.
[2] di l, taa pi, ababach r S S, al al. Keʻano o ka hoʻihoʻi houʻana no ka hoʻoholoʻana i ka haʻahaʻa haʻahaʻa. Nā lāʻau lapaʻau lāʻau lapaʻau a me ka mālamaʻana, 2012, 40 (9): 1860 - 1865.
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