New na Viro Hepatocyte Metabolism nlereanya na-eme ka usoro nwayọ -

Ebe ọ bụ na ọgwụ metanolizing enzyme nke nta nke nta na-agbada na oge ọdịnala na-enwekarị ihe dị ka 2.5 μl / cell cels), nke enweghị ike ịkọ nkọ nke ọgwụ na-eji nwayọọ^[1]. Ka ọ dị ugbu a,hepatocytes nwere ikike karie maka oxiding, mbelata, hydrolysis na igwe na-eme ka e jiri ya tụnyeremicrosomes imeju, na sistemụ vitro, ọtụtụ ụdị ọhụụ na-adịkarị nahepatocyte sistemu na n'ihu mimic na gburugburu vivo iji mee ka njigide nke enzyme ọrụhepatocyteS, na ogologo oge nke incubation oge na-enye ohere ileba anya nke ọma metabolic nke ọgwụ metabolizing^[1,2].

N'ihi nke a, iFAS, dị ka onye ndu na usoro ọmụmụ Huromax na Sypatocon ™ na usoro Sypattom ™ na usoro mgbasa ozi HepatOcon ™ na-eji mmadu ndi mmaduhepatocyteS dị ka ihe atụ, edobere usoro abụọ maka mkpebi siri ike nke ụdị ọrụ a na-ahụ maka metabolites na ụbọchị iri na ụbọchị asaa iji nyochaa usoro. Metabolite na-abawanye uru nzaghachi na ụbọchị iri na ọrụ na nkwụsi ike nke ụdị enzyme na-ekpebisi ike iji gosi na sistemụ.

• (1) Usoro nhazi usoro nhazi ọgwụ abụọ dị ka ndị a:

  IMPASE - HEPATOMAX ™Bụ COM - Cultutiontù Sistem nke dara mbà gburugburu Walles Hispacytes na sel. E mechiri Hepatocytes na mkpụrụ ndụ stromal na hepatop ™ na-eme ihe na Serrop - na metabolism nke ọgwụ na-agaghị agbanwe agbanwe. Ngụkọta nke 4, 72, awa iri na anọ na iri abụọ na anọ gachara, uru nke Metabolite ọgwụ kpebisiri ike maka nyocha.

 

  IMPASE - HEPATOCON ™N'aka nke ọzọ, onye na-eme ihe na-adịghị mma nke mmadụ na-eme ka ọ dị na Hepatocon ™. Mgbe ọ ga-emejupụta onye bụ isihepatocyteS, emechara ọgwụ ọgwụ ọgwụ mgbe ụbọchị atọ nke ọdịnala Hepatocon ™ na usoro nchịkwa na usoro akọwara ya bụ nke sistemụ Hepattomax ™.

 

Ka ọ dị ugbu a, iji hụ na izi ezi na Tourtor nke nsonaazụ, iphase rụrụ nchịkwa (cell - free) na ihe atoIMPASE - HEPATOMAX ™ naIMPASE - HEPATOCON ™ Sistemu mepụtara ezigbo nzaghachi metabolite site na ụbọchị 3 gaa n'ihu, na ha abụọ gosipụtara tụgharịa ọnụego metabolic siri ike.

 

 

• (2) Metabolism na-eme nke ọma bụ dabere na hepatocyteCyp450Sistemụ Enzyme, a ga-atụle ọrụ ya ka ọ nyochaa nke ọma.

Oseihe nwokeIMPASE - HEPATOMAX ™ naIMPASE - HEPATOCON ™ sistemụ nwere ezigbo ọrụ.

Impis guzobere otu anọ nke njikwa ọkara (enweghị sel), hepatomax ™, hepatocon ™ na sel. Na-eji Wart - otu mmaduhepatocytes dị ka ihe atụ, metabolite na-abawanye ụkpụrụ nkeCyp1a2, Cyp2b6naCyp3a4na-egosi na ụbọchị 0, 3 na 7 mgbe emecharahepatocyteIweghachite maka ụbọchị 3 na iji Flastede, Bupropion na Midiazocam dị ka mkpụrụ, n'otu n'otu. Metabolite na-abawanye ụkpụrụ gosiri na ha abụọIMPASE - HEPATOMAX ™ naIMPASE - HEPATOCON ™ Sistem gosipụtara ọrụ enzyme na-arụ ọrụ site n'ụbọchị 7, na-akwalite metabolism ọgwụ nkịtị.

 

• (3) Na mgbakwunye na nkwado nke enzyme, ezigbo nkwụsi ike bụ ihe dị mkpa na nke enweghị isi ma dị mkpa iji kwado ọrụ nke na-arụ ọrụ nke ọma.

Iphase thrase herpatomax ™, hepatocon ™ na sel ahụ na-adịghị mma iji chọpụta ihe atụ nke Tolbutimide n'ime oge ụbọchị iri asatọ mgbe a na-ahụ maka mbidohepatocytes, na nsonaazụ gosipụtara na maIMPASE - HEPATOMAX ™ naIMPASE - HEPATOCON ™ Sistem abụọ gosipụtara ọrụ kpochapụla metabolic na-aba n'anya n'ime ọnụ ahịa dị ogologo nke oge.

 

Na nchịkọta, iphase, dị ka onye ndu na vitro -Iphase hpatomax ™ naIphase hpatocon ™ na vitro models dabere na mgbidi - etinyere mmadụ isi, enwe, Canine, oke na òkéhepatocyteMkpụrụ ndụ dị iche iche nke onyinye dị iche iche, ihe omume dị mma metabolic, ihe omume enzyme na ihe omume metabolic, nke na-enye ndị ahịa ọhụrụ, ma bụrụ onye na-eme ihe dị mkpa iji ghọta nchọpụta ọgwụ! Oseihe nwokeIphase hpatomax ™ naIphase hpatocon ™ Na visro models na-enye ezigbo metabolic na-atụgharị ọnụego, ọrụ enzyme na-arụ ọrụ, na-enye ndị ahịa ọhụrụ iji zụlite ọgwụ dị ala -

 

Kwuru:

[1 1] 鞠武建, 鞠武建, 熊海伟, 低清除率药物的代谢稳定性预测模型研究进展 [中国药科大学学报. 中国药科大学学报, 2019 (2): 152 - 160.

[2] di l, trapa p, Obach r s, et al. Usoro relay ọhụrụ maka ikpebi dị ala - COCKE [J]. Metabolism na ọnọdụ, 2012, 40 (9): 1860 - 1865.