Txij li thaum cov kev ua yeeb yaj kiab tso tawm maj mam txo qis ntawm Clint Ansay yog 2.5 μl / (Mine6 hlwb)), uas tsis tuaj yeem twv seb cov tshuaj tua neeg qeeb metabolized metabolized[1]Cov. Lub caij no,Hepatocytes muaj ntau cov enzymes yuav tsum rau oxidation, txo, hydrolysis thiab kev tawm tsam dhau ntawm piv rauLier microsomes, thiab nyob rau hauv nyob rau hauv nyob rau hauv vitro nruab, ntau cov qauv tshiab zoo li tau ua kom zoo raws li covHepatocyte system thiab ntxiv rau hauv Vivo ib puag ncig kom ntev ua kom tau txais kev tuav dag zog ntawm enzyme nyob rau hauvHepatocyteS, thiab lub sijhawm ntev ntawm lub sijhawm tsim kom muaj zog muab soj ntsuam xyuas cov tshuaj metabolic txaus ntawm cov tshuaj qeeb metabolizing[1,2].
Hauv kev pom ntawm qhov no, qhov chaw lis dej num, raws li tus thawj coj hauv kev tshawb fawb txog kev ua haujlwm ntawm Hepatomax thiab nas, thiab lwm yam xov xwm tau tsim siv cov Adherent thawj tib neegHepatocyteS raws li ib qho piv txwv, thiab ob lub kaw lus tau teeb tsa kev txiav txim siab ntawm cov haujlwm thiab kev nce qib ntawm cov yeeb tshuaj tolbutamide thaum 10 hnub thiab 7 hnub los xyuas cov kab ke. Metabolite nce teb tus nqi ntawm 10 hnub thiab kev ua si thiab metabolic ruaj khov ntawm cov enzyme tshwj xeeb ntawm 7 hnub tau txiav txim siab siv lub tshuab.
- (1) Ob txoj kev siv tshuaj yeeb yaj kiab siv tau muaj raws li hauv qab no:
Ipease - hepatomax ™Yog ib co - kab lis kev cai system ntawm Khov duab walled thawj tib neeg hepatocytes thiab stromal hlwb. Thawj tus neeg Hepatocytes tau rov kho dua thiab muab cov tshuaj stromal stromal - Dawb Hepatop Of Tag nrho ntawm 4, 72, 168 thiab 240 teev tom qab tus thawj coj tshuaj, tus nqi ntxiv cov tshuaj metabolites tau txiav txim siab txog kev tsom xam.
Iphas - Hepatocon ™, ntawm qhov tod tes, yog lub system rau kev coj noj coj ua khov ua ntej tib neeg hepatocytes hauv Hepatocon ™ nruab nrab. Tom qab resuscitating tus thawj tib neegHepatocyteS, The Tshuaj Tiv Thaiv Assay tau ua tom qab 3 hnub ntawm kev coj noj coj ua hauv Hepatocon ™ nruab nrab ntawm cov txheej txheem, thiab cov txheej txheem kev coj noj coj ua thiab cov txheej txheem Assay tau zoo ib yam li Hepatomax ™ system.
Meanwhile, kom ntseeg tau qhov tseeb thiab nruj ntawm cov txiaj ntsig, Ipase ua ib qho nruab nrab tswj (ntawm peb txoj kev hloov pauv, thiab cov txiaj ntsig tau pom tias tusIpease - hepatomax ™ thiabIphas - Hepatocon ™ Cov tshuab tsim cov khoom siv muaj zog peak metabolite los ntawm ib hnub 3 tom qab, thiab nkawd ob leeg pom muaj cov khoom siv metabolic muaj zog.
- (2) Cov metabolism hauv yeeb tshuaj yog feem ntau yog nyob ntawm HepatocyteCYP450Enzyme system, thiab nws cov kev ua tuaj yeem ntsuas tau rau cov tshuaj xyuas zoo dua.
TusIpease - hepatomax ™ thiabIphas - Hepatocon ™ Cov tshuab muaj kev siv zoo.
Iphas teeb tsa plaub pawg ntawm kev tswj nruab nrab (tsis muaj hlwb), Hepatomax ™, Hepatocon ™ thiab Stromal lub hlwb. Siv phab ntsa - adherent thawj tib neegHepatocyteS raws li ib qho piv txwv, cov metabolite nce nqi rauCYP1A2, CYP2B6thiabCyp3A4tau paub tseeb thaum nruab hnub 0, 3 thiab 7 tom qabHepatocyteRov qab rau 3 hnub siv finasteride, bupropion thiab midazolam raws li cov substrates, ntsig txog. Metabolite muaj qhov tseem ceeb qhia tau tias ob qho tib siIpease - hepatomax ™ thiabIphas - Hepatocon ™ Cov txheej txheem pom kev ua haujlwm nquag ua haujlwm los ntawm Hnub 7, xwm yeem txhawb cov metabolism qub.
- (3) Ntxiv rau qhov siv tau ntawm enzyme kev ua si, kev ruaj khov metabolic ruaj kuj tseem yog ib kauj ruam tseem ceeb thiab tsis tseem ceeb hauv kev ua haujlwm hauv tsev vitro.
Iphas teeb peb pawg ntawm Hepatomax ™, Hepatocon ™, Hepatocon stability ntawm 80 hnub tau muab tshuaj 3 hnub tom qab kev rov ua haujlwm ntawm thawjHepatocytes, thiab cov txiaj ntsig tau pom tias ob tusIpease - hepatomax ™ thiabIphas - Hepatocon ™ Cov txheej txheem ob qho tib si pom tias muaj kev ua haujlwm me me ntawm cov nqi ntev tshaj plaws ntawm lub sijhawm assay.
Hauv cov ntsiab lus, cov ntsiab lus qub, ua tus thawj coj hauv cov vitro Bio - reagents, tau tsimIPASE Hepatomax ™ thiabIpase Hepatocon ™ nyob rau hauv vitro qauv raws li phab ntsa - Siv tib neeg, liab, canine, nas, nas thiab nasHepatocyteS thiab Stromal hlwb ntawm ntau yam genera, nrog cov khoom siv metabolic zoo nkauj, uas muaj cov tshuaj yeeb yaj kiab uas muaj cov kev kawm tsis meej, thiab yog ib tus khub tsis muaj zog ntxiv, thiab yog tus khub metispance tsawg), thiab yog ib tus khub metabolic kev tshem tawm, thiab yog ib tus khub metabolic kev tshem tawm, thiab yog ib tus khub metabolic kev tshem tawm, thiab yog ib tus khub metabolic zoo nkauj, thiab yog ib tus khub metabolic kev tshem tawm, thiab yog ib tus khub metabolic kev tshem tawm, thiab yog ib tus khub metabolic kev tshem tawm, thiab yog ib tus khub metabolic kev tshem tawm, thiab yog ib tus khub metabolic kev tshem tawm, thiab yog ib tus khub metabolic kev ua kom pom tseeb TusIPASE Hepatomax ™ thiabIpase Hepatocon ™ Nyob rau hauv cov qauv vitro muaj cov khoom siv venab zoo nkauj, muab cov kev xaiv ua kom zoo nkauj rau cov neeg siv khoom lag luam, thiab yog ib tus khub tseem ceeb hauv kev tshuaj!
Siv:
[1] 阮婷婷, 鞠武建, 熊海伟, 熊海伟, 等. 低清除率药物的代谢稳定性预测模型研究进展. 低清除率药物的代谢稳定性预测模型研究进展 [j]. 中国药科大学学报, 2019 (2): 152 - 160.
[2] Di L, Trapa P, Obach R S, li al. Ib txoj kev tshiab reeday rau kev txiav txim siab cov nuj nqis qis - Cov peev txheej [J]. Cov metabolism thiab cov khoom siv, 2012, 40 (9): 1860 - 1865.
Lub Sijhawm Post: 2024 - 07 - 19 17:41